Abstract
3D
hierarchical
superstructures
(3DHSs)
are
key
products
of
nature's
evolution
and
have
raised
wide
interest.
However,
the
preparation
3DHSs
composed
building
blocks
with
different
structures
is
rarely
reported,
regulating
their
structural
parameters
challenging.
Herein,
a
simple
lecithin‐mediated
biomineralization
approach
reported
for
first
time
to
prepare
gold
0D
nucleus
1D
protruding
dendritic
spikes.
It
demonstrated
that
hydrophobic
complex
by
coordination
disulfiram
(DSF)
share
chloroauric
acid
forming
3DHSs.
Under
lecithin
mediation,
reduced
form
nucleus,
followed
spike
growth
through
reduction
complex.
The
prepared
possess
well‐defined
morphology
length
≈95
nm.
Notably,
density
systematically
manipulated
from
38.9%
74.3%
controlling
DSF
concentrations.
Moreover,
diameter
regulated
9.2
12.9
nm
selecting
concentrations
tune
process.
Finite‐difference
time‐domain
(FDTD)
simulations
reveal
spikes
“hot
spots”.
dense
structure
endows
sound
performance
in
surface‐enhanced
Raman
scattering
(SERS)
applications.
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Апрель 15, 2024
Abstract
Hydrogels
are
a
class
of
highly
absorbent
and
easily
modified
polymer
materials
suitable
for
use
as
slow-release
carriers
drugs.
Gene
therapy
is
specific
can
overcome
the
limitations
traditional
tissue
engineering
techniques
has
significant
advantages
in
repair.
However,
therapeutic
genes
often
affected
by
cellular
barriers
enzyme
sensitivity,
carrier
loading
essential.
Therapeutic
gene
hydrogels
well
these
difficulties.
Moreover,
gene-therapeutic
have
made
considerable
progress.
This
review
summarizes
recent
research
on
treatment
damage
through
summary
most
current
frontiers.
We
initially
introduce
classification
their
cross-linking
methods,
followed
detailed
overview
types
modifications
genes,
discussion
characterization
features,
design
release,
an
applications
engineering.
Finally,
we
provide
comments
look
forward
to
shortcomings
future
directions
therapy.
hope
that
this
article
will
researchers
related
fields
with
more
comprehensive
systematic
strategies
repair
further
promote
development
field
Graphical
abstract
Giant,
Год журнала:
2024,
Номер
18, С. 100251 - 100251
Опубликована: Март 6, 2024
The
treatment
of
diabetic
wounds
is
a
major
challenge
faced
by
the
medical
system,
and
there
growing
interest
in
developing
innovative
therapies
to
accelerate
wound
healing.
Regenerative
medicine
with
cells
has
shown
promising
potential
skin
repair,
regenerative
properties
primarily
attributed
paracrine
effects
secreted
products,
including
exosomes.
Compared
cell-based
approaches,
using
exosomes
as
cell-free
therapy
for
chronic
several
advantages.
Exosomes
can
regulate
intercellular
communication
releasing
their
contents,
mRNA,
miRNA,
lipids,
proteins,
which
further
promote
are
well
explored
biomedical
application
owing
advantages
such
biocompatibility
low
immunogenicity.
However,
common
method
exosome
administration
through
injection,
but
due
rapid
clearance
rate
body,
maintaining
necessary
therapeutic
concentration
around
challenging.
Therefore,
it
develop
new
biocompatible
scaffold
carrier
extracellular
vesicles,
allowing
them
sustain
at
non-healing
sites
continuously
Engineered
kinds
modified
internal
treated
molecules,
surface
decoration
or
delivered
engineered
platform.
In
addition,
some
researchers
have
processed
exosomes,
known
decoration,
delivery
platforms.
regular
greater
promoting
this
review,
we
summarize
molecular
mechanisms
from
different
sources
varying
modifications
Advantages
limitations
repair
were
also
discussed.
Finally,
highlight
challenges
future
development
directions
translating
our
knowledge
into
clinical
practice.
Regenerative Therapy,
Год журнала:
2024,
Номер
26, С. 260 - 274
Опубликована: Июнь 1, 2024
Chronic
wounds
represent
a
significant
global
burden,
afflicting
millions
with
debilitating
complications.
Despite
standard
care,
impaired
healing
persists
due
to
factors
like
persistent
inflammation
and
tissue
regeneration.
Mesenchymal
stem
cell
(MSC)-derived
extracellular
vesicles
(EVs)
offer
an
innovative
regenerative
medicine
approach,
delivering
cell-derived
therapeutic
cargo
in
engineered
nanoscale
delivery
systems.
This
review
examines
pioneering
bioengineering
strategies
engineer
MSC-EVs
into
precision
nanotherapeutics
for
chronic
wounds.
Emerging
technologies
CRISPR
gene
editing,
microfluidic
manufacturing,
biomimetic
systems
are
highlighted
their
potential
enhance
MSC-EV
targeting,
optimize
enrichment,
ensure
consistent
clinical-grade
production.
However,
key
hurdles
remain,
including
batch
variability,
rigorous
safety
assessment
tumorigenicity,
immunogenicity,
biodistribution
profiling.
Crucially,
collaborative
frameworks
harmonizing
regulatory
science
patient
advocacy
hold
the
expediting
clinical
translation.
By
overcoming
these
challenges,
could
catalyze
new
era
of
off-the-shelf
therapies,
restoring
hope
afflicted
by
non-healing
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 7, 2025
Abstract
Metal‐protein
hybrid
materials
represent
a
novel
class
of
functional
that
exhibit
exceptional
physicochemical
properties
and
tunable
structures,
rendering
them
remarkable
applications
in
diverse
fields,
including
engineering,
biocatalysis,
biosensing,
biomedicine.
The
design
development
multifunctional
biocompatible
metal‐protein
have
been
the
subject
extensive
research
key
aspiration
for
practical
clinical
settings.
This
review
provides
comprehensive
analysis
strategies,
intrinsic
properties,
biomedical
these
materials,
with
specific
emphasis
on
their
potential
cancer
therapy,
drug
vaccine
delivery,
antibacterial
treatments,
tissue
regeneration.
Through
rational
design,
stable
can
be
synthesized
using
straightforward
methods,
enabling
therapeutic,
immunomodulatory,
other
desired
functionalities.
Finally,
outlines
existing
limitations
challenges
associated
evaluates
translation,
providing
insights
into
implementation
within
applications.
Abstract
Diabetic
wounds
are
among
the
most
common
complications
of
diabetes
mellitus
and
their
healing
process
can
be
delayed
due
to
persistent
inflammatory
reactions,
bacterial
infections,
damaged
vascularization
impaired
cell
proliferation,
which
casts
a
blight
on
patients’health
quality
life.
Therefore,
new
strategies
accelerate
diabetic
wound
being
positively
explored.
Exosomes
derived
from
mesenchymal
stem
cells
(MSC-Exos)
inherit
therapeutic
reparative
abilities
play
crucial
role
in
healing.
However,
poor
targeting,
low
concentrations
molecules,
easy
removal
limited
yield
MSC-Exos
challenging
for
clinical
applications.
Bioengineering
techniques
have
recently
gained
attention
ability
enhance
efficacy
MSC-Exos.
In
this
review,
we
summarise
focus
three
bioengineering
strategies,
namely,
parental
engineering,
direct
engineering
combined
with
biomaterials.
Furthermore,
application
bioengineered
is
reviewed.
Finally,
discuss
future
prospects
MSC-Exos,
providing
insights
into
exploration
strategies.
ACS Applied Nano Materials,
Год журнала:
2024,
Номер
7(10), С. 11037 - 11052
Опубликована: Май 15, 2024
Diabetic
wound
infection
has
always
been
a
huge
threat
to
human's
healthcare,
and
most
of
the
existing
therapies
have
reduced
efficacy,
drug
resistance
toxic
side
effects.
Therefore,
there
is
an
urgent
need
develop
innovative
therapeutic
strategies
for
diabetic
management.
At
present,
intelligence
stimulus-responsive
bioactive
hydrogels
in
healing
received
extensive
attention.
This
mainly
reflected
on-demand
release
active
substances
response
physical,
chemical
or
biological
stimuli.
paper
focuses
on
potential
various
their
applications
healing,
briefly
discusses
factors
slow
preparation
methods
smart
hydrogels.
Finally,
future
development
further
management
wounds
presented.
