A Hemoglobin Bionics‐Based System for Combating Antibiotic Resistance in Chronic Diabetic Wounds via Iron Homeostasis Regulation

Advanced Materials, Год журнала: 2024, Номер 36(30)

Опубликована: Май 13, 2024

Owing to the increased tissue iron accumulation in patients with diabetes, microorganisms may activate high expression of iron-involved metabolic pathways, leading exacerbation bacterial infections and disruption systemic glucose metabolism. Therefore, an on-demand transdermal dosing approach that utilizes homeostasis regulation combat antimicrobial resistance is a promising strategy address challenges associated low administration bioavailability antibiotic treating infected diabetic wounds. Here, it aimed propose effective therapy based on hemoglobin bionics induce disturbances homeostasis. The preferred "iron cargo" synthesized by protoporphyrin IX chelated dopamine gallium (PDGa), delivered via glucose/pH-responsive microneedle bandage (PDGa@GMB). PDGa@GMB downregulates levels uptake regulator (Fur) peroxide response (perR) Staphylococcus aureus, nutrient starvation oxidative stress, ultimately suppressing iron-dependent activities. Consequently, demonstrates insusceptibility genetic while maintaining sustainable effects (>90%) against resistant strains both S. aureus E. coli, accelerates recovery (<20 d). Overall, not only counteracts but also holds tremendous potential mediating microbial-host crosstalk, synergistically attenuating pathogen virulence pathogenicity.

Язык: Английский

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Structural Color Colloidal Photonic Crystals for Biomedical Applications

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Июль 31, 2024

Photonic crystals are a new class of optical microstructure materials characterized by dielectric constant that varies periodically with space and features photonic bandgap. Inspired natural such as butterfly scales, series artificial developed for use in integrated platforms, biosensing, communication, other fields. Among them, colloidal (CPCs) have gained widespread attention due to their excellent properties advantages, ease preparation functionalization. This work reviews the classification self-assembly principles CPCs, details some latest biomedical applications large-area, high-quality CPCs prepared using advanced methods, summarizes existing challenges CPC construction application, anticipates future development directions optimization strategy. With further advancements, expected play more critical role biosensors, drug delivery, cell research, fields, bringing significant benefits research clinical practice.

Язык: Английский

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A Versatile Cryomicroneedle Patch for Traceable Photodynamic Therapy

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Май 27, 2024

Abstract Photodynamic therapy (PDT) continues to encounter multifarious hurdles, stemming from the ineffectual preservation and delivery system of photosensitizers, dearth imaging navigation, antioxidant/hypoxic tumor microenvironment. Herein, a versatile cryomicroneedle patch (denoted as CMN‐CCPH) is developed for traceable PDT. The therapeutic efficacy further amplified by catalase (CAT)‐induced oxygen (O 2 ) generation Cu 2+ ‐mediated glutathione (GSH) depletion. CMN‐CCPH composed (CMN) vehicle CAT‐biomineralized copper phosphate nanoflowers (CCP NFs) loaded with hematoporphyrin monomethyl ether (HMME) payload. Importantly, bioactive function HMME CAT can be optimally maintained under protection CCPH CMN duration surpassing 60 days, leading bolstered bioavailability notable enhancements in PDT efficacy. vivo visualization oxyhemoglobin saturation (sO monitored fluorescence (FL)/photoacoustic (PA) duplex real‐time unveils noteworthy implications CMN‐delivered intratumoral enrichment O reduced systemic toxicity. This demonstrates distinct effectiveness neoplasm elimination, underscoring its promising clinical prospects.

Язык: Английский

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A dual-action strategy: Wound microenvironment responsive hydrogel and exosome-mediated glucose regulation enhance inside-out diabetic wound repair

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113716 - 113716

Опубликована: Апрель 1, 2025

Язык: Английский

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MicroSphere 3D Structures Delay Tissue Senescence through Mechanotransduction

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

The extracellular matrix (ECM) stores signaling molecules and facilitates mechanical biochemical in cells. However, the influence of biomimetic "rejuvenation" ECM structures on aging- degeneration-related cellular activities tissue repair is not well understood. We combined physical extrusion precise "on–off" alternating cross-linking methods to create anisotropic biomaterial microgels (MicroRod MicroSphere) explored how they regulate cell nucleus pulposus (NP) their potential antidegenerative effects intervertebral discs. NP cells exhibited aligned growth along surface MicroRod, enhanced proliferation, reduced apoptosis. This suggests an adaptive response involving adhesion mechanosensing, which causes cytoskeletal extension via environmental cues. maintain nuclear membrane integrity through YAP/TAZ pathway, activates cGAS-STING pathway rectify aging mechanisms. In vivo, MicroRod carries reduces inflammatory factor protease secretion degenerated discs, inhibiting degeneration promoting regeneration. Our findings highlight role stress maintaining activity antiaging harsh environments, providing a foundation for further research development biomaterials.

Язык: Английский

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Andrias davidianus Derived Glycosaminoglycans Direct Diabetic Wound Repair by Reprogramming Reparative Macrophage Glucolipid Metabolism

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

Abstract Harnessing cross‐species regenerative cues to direct human potential is increasingly recognized as an excellent strategy in medicine, particularly for addressing the challenges of impaired wound healing aging populations. The skin mucus Andrias davidianus plays a critical role self‐protection and tissue repair, yet fundamental factors mechanisms involved remain elusive. Here, this work presents evidence that glycosaminoglycans (GAGs) derived from secretion (SAGs) serve potent mediators angiogenesis inflammatory remodeling, facilitating efficient diabetic wounds. Mechanistic studies reveal SAGs promote macrophage polarization toward anti‐inflammatory pro‐regenerative phenotype (CD206 + /Arg1 ) via glucolipid metabolic reprogramming. This process suppresses excessive inflammation enhances expression VEGF IL‐10 create facilitative microenvironment regeneration. Additionally, develops SAGs‐GelMA composite microspheres address multiple stages healing, including rapid hemostasis, exudate control, activation endogenous processes. engineered approach significantly improves scarless wounds by recruitment reparative macrophages. findings offer new insights into highlight therapeutic application repair.

Язык: Английский

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Novel microneedle platforms for the treatment of wounds by drug delivery: A review

Colloids and Surfaces B Biointerfaces, Год журнала: 2023, Номер 233, С. 113636 - 113636

Опубликована: Ноя. 11, 2023

Язык: Английский

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Cuttlefish‐Inspired Photo‐Responsive Antibacterial Microparticles with Natural Melanin Nanoparticles Spray

Small, Год журнала: 2023, Номер 20(19)

Опубликована: Дек. 5, 2023

Abstract Topical antibiotics can be utilized to treat periodontitis, while their delivery stratagems with controlled release and long‐lasting bactericidal inhibition are yet challenging. Herein, inspired by the defensive behavior of cuttlefish expelling ink, this work develops innovative thermal‐responsive melanin‐integrated porous microparticles (MPs) through microfluidic synthesis for periodontitis treatment. These MPs composed melanin nanoparticles (NPs), poly(N‐isopropylacrylamide) (PNIPAM), agarose. Benefiting from excellent biocompatibility large surface area ratio MPs, they deliver abundant NPs. Under near‐infrared irradiation, NPs convert photo energy into thermal energy. This leads agarose melting subsequent shrinkage microspheres induced pNIPAM, thereby facilitating In addition, released serve as a highly effective photothermal agent, displaying potent antibacterial activity against porphyromonas gingivalis possessing natural anti‐inflammatory properties. unique characteristics further demonstrated in vivo experiments, showing effects treatment infected wounds periodontitis. Therefore, catfish‐inspired photo‐responsive controlled‐release drug hold tremendous potential clinical applications.

Язык: Английский

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Novel lipid nanovesicle-loaded dissolving microarray patches for fenretinide in breast cancer chemoprevention

Journal of Controlled Release, Год журнала: 2024, Номер 374, С. 76 - 88

Опубликована: Авг. 10, 2024

The retinoid fenretinide (FENR) is a promising compound for preventing breast cancer recurrence but faces challenges due to poor solubility and low bioavailability. This study explores the development of dissolving microneedles (MNs) containing FENR-loaded ethosomes minimally invasive chemoprevention, aiming enhance local drug distribution. Ethosomes were formulated using ethanol, propylene glycol, soya lecithin, water, polysorbate 80 micelles. MNs created from poly(vinyl alcohol) poly(vinylpyrrolidone) hydrogels by adding polymer powder directly into suspensions, reducing manufacturing time cost. Two methods used load high-density moulds: 1) only in needle area, 2) both area baseplate. Dynamic light scattering confirmed nanostructures MNs. Micelle-based dissolved 15 min, compared 30 min other Skin deposition studies showed greater (up 10 μg/patch) enhanced skin permeation FENR 40 μg) with Method 2. In-vivo rats demonstrated that oral administration resulted plasma levels below ng/g first three hours, whereas MN delayed delivery, reaching maximum concentration 52 at 48 h. decreased 3 μg/g on day 1 <0.3 last day. indicates are potential dosage form delivering FENR, offering new approach chemoprevention.

Язык: Английский

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Biological scaffold as potential platforms for stem cells: Current development and applications in wound healing

World Journal of Stem Cells, Год журнала: 2024, Номер 16(4), С. 334 - 352

Опубликована: Апрель 24, 2024

Wound repair is a complex challenge for both clinical practitioners and researchers. Conventional approaches wound have several limitations. Stem cell-based therapy has emerged as novel strategy to address this issue, exhibiting significant potential enhancing healing rates, improving quality, promoting skin regeneration. However, the use of stem cells in regeneration presents challenges. Recently, biomaterials been identified crucial components wound-healing process. Combination involving development biocompatible scaffolds, accompanying cells, multiple biological factors, structures resembling natural extracellular matrix (ECM) gained considerable attention. Biological scaffolds encompass range that serve platforms seeding providing them with an environment conducive growth, similar ECM. These facilitate delivery application tissue healing. This article provides comprehensive review current developments applications healing, emphasizing their capacity cell adhesion, proliferation, differentiation, paracrine functions. Additionally, we identify pivotal characteristics contribute enhanced cellular activity.

Язык: Английский

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