Bioinspired gradient scaffolds for osteochondral tissue engineering

Exploration, Год журнала: 2023, Номер 3(4)

Опубликована: Июль 12, 2023

Repairing articular osteochondral defects present considerable challenges in self-repair due to the complex tissue structure and low proliferation of chondrocytes. Conventional clinical therapies have not shown significant efficacy, including microfracture, autologous/allograft transplantation, cell-based techniques. Therefore, engineering has been widely explored repairing by leveraging natural regenerative potential biomaterials control cell functions. However, is a gradient with smooth transition from cartilage subchondral bone, involving changes chondrocyte morphologies phenotypes, extracellular matrix components, collagen type orientation, cytokines. Bioinspired scaffolds developed simulating characteristics heterogeneous tissues, such as pores, osteochondrogenesis-inducing factors, satisfy anisotropic features matrices. repair altering microenvironments growth induce osteochondrogenesis promote formation interfaces compared homogeneous scaffolds. This review outlines meaningful strategies for based on predicts pros cons prospective translation into practice.

Язык: Английский

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Advances of calcium phosphate nanoceramics for the osteoinductive potential and mechanistic pathways in maxillofacial bone defect repair

Nano TransMed, Год журнала: 2024, Номер 3, С. 100033 - 100033

Опубликована: Фев. 4, 2024

Maxillofacial bone defects, arising from congenital malformations, tumors, infections, and traumas, represent a common clinical condition that impairs patients' appearance, speech, motor functions. Calcium phosphate nanoceramics, which bear chemical composition similar to autologous bone, demonstrate strong biocompatibility osteoconductivity, making them an extensively applied material in defect repairing. Notably, these nanoceramics exhibit inherent osteoinductive properties ,which can stimulate osteogenesis at non-osseous sites ,without the need for additional cells or growth factors, aspects further expand their application repair. Nevertheless, precise mechanism of calcium remains elusive, differences vivo individual persist, thereby academic communities resolve pressing issues restrict use. This paper reviews advancements research on factors affecting biological process formation, aiming provide new insights treatment maxillofacial defects.

Язык: Английский

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Biomimetic Hydrogel Containing Copper Sulfide Nanoparticles and Deferoxamine for Photothermal Therapy of Infected Diabetic Wounds

Advanced Healthcare Materials, Год журнала: 2023, Номер 13(8)

Опубликована: Дек. 8, 2023

Abstract Inducing cell migration from the edges to center of a wound, promoting angiogenesis, and controlling bacterial infection are very important for diabetic wound healing. Incorporating growth factors antibiotics into hydrogels dressing is considered potential strategy meet these requirements. However, some present drawbacks greatly slow down their development toward application, such as short half‐life high price factors, low antibiotic efficiency against drug‐resistant bacteria, insufficient ability promote migration, etc. Deferoxamine (DFO) can upregulate expression HIF‐1α, thus stimulating secretion angiogenesis‐related endogenous factors. Copper sulfide (CuS) nanoparticles possess excellent antibacterial performance combined with photothermal therapy (PTT). Herein, DFO CuS incorporated biomimetic hydrogel, which mimics structure function extracellular matrix (ECM), abbreviated DFO/CuS‐ECMgel. This hydrogel expected be able adhesion degraded by cell‐secreted metalloproteinases (MMPs), then release at site exert therapeutic effects. As result, three crucial requirements healing, “beneficial effectively killing bacteria,” achieved simultaneously.

Язык: Английский

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3D printing in biomedicine: advancing personalized care through additive manufacturing

Exploration of Medicine, Год журнала: 2023, Номер unknown, С. 1135 - 1167

Опубликована: Дек. 29, 2023

The integration of three-dimensional (3D) printing techniques into the domains biomedical research and personalized medicine highlights evolving paradigm shifts within contemporary healthcare. This technological advancement signifies potential breakthroughs in patient-specific therapeutic interventions innovations. systematic review offers a critical assessment existing literature, elucidating present status, inherent challenges, prospective avenues 3D augmenting applications formulating tailored medical strategies. Based on an exhaustive literature analysis comprising empirical studies, case extensive reviews from past decade, pivotal sectors including tissue engineering, prosthetic development, drug delivery systems, customized apparatuses are delineated. advent provides precision fabrication patient-centric implants, bio-structures, devices, thereby mitigating associated risks. Concurrently, it facilitates ideation individualized paradigms to optimize outcomes. Notwithstanding these advancements, issues concerning material biocompatibility, regulatory compliance, economic implications avant-garde persist. To fully harness transformative healthcare, collaborative endeavors amongst academicians, clinicians, industrial entities, bodies paramount. With continued innovation, is poised redefine trajectories science care. paper aims justify objective whether what extent technology biomedicine enhances treatment contributes improved healthcare

Язык: Английский

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Advances in volumetric bioprinting

Biofabrication, Год журнала: 2023, Номер 16(1), С. 012004 - 012004

Опубликована: Ноя. 3, 2023

The three-dimensional (3D) bioprinting technologies are suitable for biomedical applications owing to their ability manufacture complex and high-precision tissue constructs. However, the slow printing speed of current layer-by-layer (bio)printing modality is major limitation in biofabrication field. To overcome this issue, volumetric (VBP) developed. VBP changes layer-wise operation conventional devices, permitting creation geometrically complex, centimeter-scale constructs tens seconds. next step onward from sequential methods, opening new avenues fast additive manufacturing fields engineering, regenerative medicine, personalized drug testing, soft robotics, etc. Therefore, review introduces principles hardware designs VBP-based techniques; then focuses on recent advances (bio)inks applications. Lastly, limitations discussed together with future direction research.

Язык: Английский

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Organic–inorganic composite hydrogels: compositions, properties, and applications in regenerative medicine

Biomaterials Science, Год журнала: 2024, Номер 12(5), С. 1079 - 1114

Опубликована: Янв. 1, 2024

Hydrogels, formed from crosslinked hydrophilic macromolecules, provide a three-dimensional microenvironment that mimics the extracellular matrix. They served as scaffold materials in regenerative medicine with an ever-growing demand. However, hydrogels composed of only organic components may not fully meet performance and functionalization requirements for various tissue defects. Composite hydrogels, containing inorganic components, have attracted tremendous attention due to their unique compositions properties. Rigid particles, rods, fibers,

Язык: Английский

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Self-Assembling Peptide Nanofibers Anchored Parathyroid Hormone Derivative for Bone Tissue Engineering

Advanced Fiber Materials, Год журнала: 2024, Номер 6(2), С. 583 - 606

Опубликована: Фев. 21, 2024

Язык: Английский

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Microenvironment Remodeling Self-Healing Hydrogel for Promoting Flap Survival

Biomaterials Research, Год журнала: 2024, Номер 28

Опубликована: Янв. 1, 2024

Random flap grafting is a routine procedure used in plastic and reconstructive surgery to repair reconstruct large tissue defects. Flap necrosis primarily caused by ischemia–reperfusion injury inadequate blood supply the distal flap. Ischemia–reperfusion leads production of excessive reactive oxygen species, creating pathological microenvironment that impairs cellular function angiogenesis. In this study, we developed remodeling self-healing hydrogel [laminarin–chitosan-based hydrogel-loaded extracellular vesicles ceria nanozymes (LCH@EVs&CNZs)] improve synergistically promote regeneration survival. The natural (LCH) was created oxidation laminarin carboxymethylated chitosan via Schiff base reaction. We loaded with CNZs EVs. are class nanomaterials enzymatic activity known for their strong scavenging capacity thus alleviating oxidative stress. EVs cell-secreted vesicular structures containing thousands bioactive substances can cell proliferation, migration, differentiation, constructed LCH@EVs&CNZs demonstrated robust excess thereby conferring protection stress environments. Moreover, these constructs notably enhance migration Our results demonstrate effectively remodel skin marked This approach introduces new therapeutic strategy combining microenvironmental EV therapy, which holds promise promoting

Язык: Английский

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Bioengineered Silk Protein‐Based 3D In Vitro Models for Tissue Engineering and Drug Development: From Silk Matrix Properties to Biomedical Applications

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(28)

Опубликована: Июль 15, 2024

3D in vitro model has emerged as a valuable tool for studying tissue development, drug screening, and disease modeling. systems can accurately replicate microstructures physiological features, mirroring the vivo microenvironment departing from conventional 2D cell cultures. Various models utilizing biomacromolecules like collagen synthetic polymers have been developed to meet diverse research needs address complex challenges of contemporary research. Silk proteins, bearing structural functional similarities collagen, increasingly employed construct advanced systems, surpassing limitations This review examines silk proteins' composition, structure, properties, functions, elucidating their role models. Furthermore, recent advances biomedical applications involving silk-based organoid are discussed. In particular, unique attributes matrix constituents constructs highlighted, providing meticulous evaluation importance. Additionally, it outlines current hurdles complexities while contemplating future avenues, thereby paving way developing biomimetic protein-based microtissues.

Язык: Английский

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Highly Flexible Methyl Cellulose/Gelatin Hydrogels for Potential Cartilage Tissue Engineering Applications

Biopolymers, Год журнала: 2025, Номер 116(1)

Опубликована: Янв. 1, 2025

ABSTRACT Cartilage damage resulting from trauma demonstrates a poor capacity for repair due to its avascular nature. tissue engineering offers unique therapeutic option cartilage recovery. In this study, methylcellulose (MC)/gelatin (GEL) hydrogels (MC10G20, MC12.5G20, MC15G20, and MC17.5G20) were developed assess compare their chemical, mechanical, biological characteristics repair. First, the interaction between MC GEL after blending subsequent crosslinking with EDC/NHS was confirmed by using FTIR. Mechanical tests under compression test revealed that hydrogels' resistance both elastic plastic deformation increased higher wt.% of MC. The % strain doubled addition MC, likely abundant hydrogen bonding polymeric chains. Furthermore, compressive modulus MC/GEL approximately 0.2 MPa, closely matching human tissue. Similarly, water retention over 7 days as content increased. Additionally, SEM images showed incorporation introduced porosity diameters ranging 10 50 μm, similar size pores in native cartilage. vitro cell culture studies biocompatibility hydrogels. Fluorescence staining 2.5‐fold increase F‐actin following into Overall, study highlights potential engineering, however, further research is required full potential.

Язык: Английский

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