Methods to Discover and Validate Biofluid-Based Biomarkers in Neurodegenerative Dementias

Molecular & Cellular Proteomics, Год журнала: 2023, Номер 22(10), С. 100629 - 100629

Опубликована: Авг. 8, 2023

Neurodegenerative dementias are progressive diseases that cause neuronal network breakdown in different brain regions often because of accumulation misfolded proteins the extracellular matrix, such as amyloids or inside neurons other cell types brain. Several diagnostic protein biomarkers body fluids being used and implemented, for Alzheimer's disease. However, there is still a lack co-pathologies causes dementia. Such biofluid-based enable precision medicine approaches diagnosis treatment, allow to learn more about underlying disease processes, facilitate development patient inclusion evaluation tools clinical trials. When designing studies discover novel biomarkers, choice technology an important starting point. But so many technologies choose among. To address this, we here review currently available research settings and, some cases, laboratory practice. This presents form lexicon on each addressing its use clinics, strengths limitations, future perspective.

Язык: Английский

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Quantitative proteomic analysis of skeletal muscles from wild-type and transgenic mice carrying recessive Ryr1 mutations linked to congenital myopathies

eLife, Год журнала: 2023, Номер 12

Опубликована: Март 2, 2023

Skeletal muscles are a highly structured tissue responsible for movement and metabolic regulation, which can be broadly subdivided into fast slow twitch with each type expressing common as well specific sets of proteins. Congenital myopathies group muscle diseases leading to weak phenotype caused by mutations in number genes including RYR1. Patients carrying recessive RYR1 usually present from birth generally more severely affected, showing preferential involvement extraocular facial muscles. In order gain insight the pathophysiology RYR1-congential myopathies, we performed relative absolute quantitative proteomic analysis skeletal wild-type transgenic mice p.Q1970fsX16 p.A4329D RyR1 were identified child severe congenital myopathy. Our in-depth shows that not only decrease content protein muscle, but change expression 1130, 753, 967 proteins EDL, soleus muscles, respectively. Specifically, affect level involved calcium signaling, extracellular matrix, metabolism ER quality control. This study also reveals stoichiometry major excitation contraction coupling identifies novel potential pharmacological targets treat RyR1-related myopathies.

Язык: Английский

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Cell membrane coated nanoparticles as a biomimetic drug delivery platform for enhancing cancer immunotherapy

Nanoscale, Год журнала: 2024, Номер 16(18), С. 8708 - 8738

Опубликована: Янв. 1, 2024

Cancer immunotherapy, a burgeoning modality for cancer treatment, operates by activating the autoimmune system to impede growth of malignant cells. Although numerous immunotherapy strategies have been employed in clinical therapy, resistance cells immunotherapeutic medications and other apprehensions attainment sustained advantages most patients. Recent advancements nanotechnology drug delivery hold promise augmenting efficacy immunotherapy. However, is currently constrained inadequate specificity delivery, low rate response, intricate immunosuppressive tumor microenvironment. In this context, investigation cell membrane coated nanoparticles (CMNPs) has revealed their ability perform targeted immune evasion, controlled release, immunomodulation. By combining advantageous features natural membranes nanoparticles, CMNPs demonstrated unique potential realm This review aims emphasize recent research progress elucidate underlying mechanisms as an innovative platform enhancing Additionally, it provides comprehensive overview current involving different types CMNPs, with intention further exploration optimization.

Язык: Английский

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Critical review of new advances in food and plant proteomics analyses by nano-LC/MS towards advanced foodomics

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 176, С. 117759 - 117759

Опубликована: Май 14, 2024

Язык: Английский

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From bench to bedside: Platelet biomimetic nanoparticles as a promising carriers for personalized drug delivery

Journal of Controlled Release, Год журнала: 2024, Номер 373, С. 128 - 144

Опубликована: Июль 14, 2024

Язык: Английский

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Analytical methods for the analysis of bromoform in red seaweed Asparagopsis armata and Asparagopsis taxiformis – A review

Algal Research, Год журнала: 2024, Номер 79, С. 103478 - 103478

Опубликована: Март 19, 2024

Global warming is a significant risk to all species on this planet and exacerbated by the release of greenhouse gases (GHG) into atmosphere. Agricultural practices represent >20 % total GHG emissions with 14.6 being ruminant livestock farming alone. The red seaweed Asparagopsis taxiformis armata are source bioactive substances including brominated compounds that have been shown reduce methane emission up 98 when supplemented feeding regimes. Bromoform major contributor reduction in methanogenesis can be found remarkably high concentrations 1–5 dry mass species. toxicity bromoform makes it essential for emerging industry accurately quantify Asparagopsis. quantification normally conducted hyphenated gas chromatography system due its volatility routine methods established over 60 years water air. complexity algal matrix provides challenge researchers alike extract manner safe, green, cost-effective reflective actual concentration. This review summarises instrumentation methods, non-chromatography potentially useful quantification, sample preparation in-field analysis, storage stability seaweed.

Язык: Английский

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Gel electrophoresis-based proteoform separation and analysis

Advances in clinical chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Deciphering Biofilm Formation in Veterinary Pathogens: Unveiling Insights Through Proteomics Analysis

Livestock diseases and management, Год журнала: 2025, Номер unknown, С. 447 - 471

Опубликована: Янв. 1, 2025

Язык: Английский

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Characterization of immune features and discovery of potential biomarkers for ankylosing spondylitis using deep plasma proteomics

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Ankylosing spondylitis (AS) is a systemic inflammatory disorder that predominantly involves the axial skeleton, often leading to irreversible structural damage and disability. Although several therapeutic measurements are available, limitations in efficacy long-term outcomes remain significant. Therefore, identifying novel biomarkers targets of critical importance for optimizing clinical management prognostic evaluation AS patients. This study aims elucidate immune features discover potential by integration deep plasma proteomics learning strategies. The quantitative was applied analyze samples from 104 participants patients with active stable stages, along healthy controls. functional different stages were assessed. By integrating random forest (RF) orthogonal partial least squares discriminant analysis (OPLS-DA), machine model-based score matrix constructed identify biomarkers. ELISA experiments performed on an independent cohort 79 confirm AS. Patients exhibit significant dysregulation distributions characteristics cells. Several key proteins involved integrin signaling pathway significantly differentially expressed AS, highlighting pathway's role pathogenesis Four including SAA1, FERMT3, ILK, TLN1, identified as further verified experiments. learning-based method analysis, we explored pathological mechanism Our provides insights into distinct protein expression patterns may contribute diagnosis, monitoring, therapy this disease.

Язык: Английский

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Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics

Biomolecules, Год журнала: 2023, Номер 13(1), С. 96 - 96

Опубликована: Янв. 3, 2023

The relatively recent developments in mass spectrometry (MS) have provided novel opportunities for this technology to impact modern medicine. One of those is biomarker discovery and diagnostics. Key sample preparation enabled a greater range clinical samples be characterized at deeper level using MS. While most these focused on blood, tissues also been an important resource. Fresh tissues, however, are difficult obtain research purposes require significant resources long-term storage. There millions archived formalin-fixed paraffin-embedded (FFPE) within pathology departments worldwide representing every possible tissue type including tumors that rare or very small. Owing the chemical technique used preserve FFPE they were considered intractable many newer proteomics techniques primarily only useful immunohistochemistry. In past couple decades, researchers able develop methods extract proteins from form making them analyzable state-of-the-art technologies such as MS protein arrays. This review will discuss history provide examples how currently being identify biomarkers diagnose diseases cancer.

Язык: Английский

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