Simultaneous Determination of Animal Products from Ruminant, Pig, Poultry, and Fish in Feedingstuff by Targeted High-Resolution LC–MS/MS DOI

Sara Schlachter,

Klaudia Tadus,

Roland Weiß

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(27), P. 15357 - 15365

Published: June 28, 2024

The prohibition of processed animal proteins (PAPs) has been relaxed gradually since 2007. official control method for PAPs in feedingstuff, a combination light microscopy (LM) followed by PCR, is no longer sufficient. Thus, targeted LC–MS/MS was developed, which enables tissue-specific distinction between egg and dairy products, gelatine, derived from blood or muscle tissue the species ruminants, pigs, poultry, fish. Tissue-specific were analyzed after tryptic digestion to peptides with high-resolution ESI-QTOF-MS. A developed based on untargeted proteomics approaches selection specific (45 unique total). Proficiency testing blank spiked samples revealed excellent results trueness selectivity. Furthermore, sensitivity achieved at level 0.1% (w/w) assessed peptides. Summing up, seems be suitable routine analysis verification ring trials.

Language: Английский

Methods to Discover and Validate Biofluid-Based Biomarkers in Neurodegenerative Dementias DOI Creative Commons
Charlotte E. Teunissen, Leighann Kimble, Sherif Bayoumy

et al.

Molecular & Cellular Proteomics, Journal Year: 2023, Volume and Issue: 22(10), P. 100629 - 100629

Published: Aug. 8, 2023

Neurodegenerative dementias are progressive diseases that cause neuronal network breakdown in different brain regions often because of accumulation misfolded proteins the extracellular matrix, such as amyloids or inside neurons other cell types brain. Several diagnostic protein biomarkers body fluids being used and implemented, for Alzheimer's disease. However, there is still a lack co-pathologies causes dementia. Such biofluid-based enable precision medicine approaches diagnosis treatment, allow to learn more about underlying disease processes, facilitate development patient inclusion evaluation tools clinical trials. When designing studies discover novel biomarkers, choice technology an important starting point. But so many technologies choose among. To address this, we here review currently available research settings and, some cases, laboratory practice. This presents form lexicon on each addressing its use clinics, strengths limitations, future perspective.

Language: Английский

Citations

22
Quantitative proteomic analysis of skeletal muscles from wild-type and transgenic mice carrying recessive Ryr1 mutations linked to congenital myopathies DOI Creative Commons

Jan Eckhardt,

Alexis Ruiz, Stéphane Koenig

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: March 2, 2023

Skeletal muscles are a highly structured tissue responsible for movement and metabolic regulation, which can be broadly subdivided into fast slow twitch with each type expressing common as well specific sets of proteins. Congenital myopathies group muscle diseases leading to weak phenotype caused by mutations in number genes including RYR1. Patients carrying recessive RYR1 usually present from birth generally more severely affected, showing preferential involvement extraocular facial muscles. In order gain insight the pathophysiology RYR1-congential myopathies, we performed relative absolute quantitative proteomic analysis skeletal wild-type transgenic mice p.Q1970fsX16 p.A4329D RyR1 were identified child severe congenital myopathy. Our in-depth shows that not only decrease content protein muscle, but change expression 1130, 753, 967 proteins EDL, soleus muscles, respectively. Specifically, affect level involved calcium signaling, extracellular matrix, metabolism ER quality control. This study also reveals stoichiometry major excitation contraction coupling identifies novel potential pharmacological targets treat RyR1-related myopathies.

Language: Английский

Citations

20
Critical review of new advances in food and plant proteomics analyses by nano-LC/MS towards advanced foodomics DOI
Cemil Aydoğan

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 176, P. 117759 - 117759

Published: May 14, 2024

Language: Английский

Citations

8
From bench to bedside: Platelet biomimetic nanoparticles as a promising carriers for personalized drug delivery DOI
Ammara Safdar,

Peina Wang,

Abdul Muhaymin

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 373, P. 128 - 144

Published: July 14, 2024

Language: Английский

Citations

7
Cell membrane coated nanoparticles as a biomimetic drug delivery platform for enhancing cancer immunotherapy DOI

Zichen Zhong,

Wen Deng,

Jian Wu

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(18), P. 8708 - 8738

Published: Jan. 1, 2024

Cancer immunotherapy, a burgeoning modality for cancer treatment, operates by activating the autoimmune system to impede growth of malignant cells. Although numerous immunotherapy strategies have been employed in clinical therapy, resistance cells immunotherapeutic medications and other apprehensions attainment sustained advantages most patients. Recent advancements nanotechnology drug delivery hold promise augmenting efficacy immunotherapy. However, is currently constrained inadequate specificity delivery, low rate response, intricate immunosuppressive tumor microenvironment. In this context, investigation cell membrane coated nanoparticles (CMNPs) has revealed their ability perform targeted immune evasion, controlled release, immunomodulation. By combining advantageous features natural membranes nanoparticles, CMNPs demonstrated unique potential realm This review aims emphasize recent research progress elucidate underlying mechanisms as an innovative platform enhancing Additionally, it provides comprehensive overview current involving different types CMNPs, with intention further exploration optimization.

Language: Английский

Citations

6
Analytical methods for the analysis of bromoform in red seaweed Asparagopsis armata and Asparagopsis taxiformis – A review DOI Creative Commons

Joshua L. Hutchings,

Yevgeniya Grebneva,

Sarah J. Dilmetz

et al.

Algal Research, Journal Year: 2024, Volume and Issue: 79, P. 103478 - 103478

Published: March 19, 2024

Global warming is a significant risk to all species on this planet and exacerbated by the release of greenhouse gases (GHG) into atmosphere. Agricultural practices represent >20 % total GHG emissions with 14.6 being ruminant livestock farming alone. The red seaweed Asparagopsis taxiformis armata are source bioactive substances including brominated compounds that have been shown reduce methane emission up 98 when supplemented feeding regimes. Bromoform major contributor reduction in methanogenesis can be found remarkably high concentrations 1–5 dry mass species. toxicity bromoform makes it essential for emerging industry accurately quantify Asparagopsis. quantification normally conducted hyphenated gas chromatography system due its volatility routine methods established over 60 years water air. complexity algal matrix provides challenge researchers alike extract manner safe, green, cost-effective reflective actual concentration. This review summarises instrumentation methods, non-chromatography potentially useful quantification, sample preparation in-field analysis, storage stability seaweed.

Language: Английский

Citations

5
Deciphering Biofilm Formation in Veterinary Pathogens: Unveiling Insights Through Proteomics Analysis DOI

Anati Abd Rashid Syaida,

Mohd Taufiq Mat Jalil, Shafiq Aazmi

et al.

Livestock diseases and management, Journal Year: 2025, Volume and Issue: unknown, P. 447 - 471

Published: Jan. 1, 2025

Language: Английский

Citations

0
Biomarker Analysis of Formalin-Fixed Paraffin-Embedded Clinical Tissues Using Proteomics DOI Creative Commons

N. Ekenedirichukwu,

Daniel A. Tellock,

Gabriel J. Thomas

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(1), P. 96 - 96

Published: Jan. 3, 2023

The relatively recent developments in mass spectrometry (MS) have provided novel opportunities for this technology to impact modern medicine. One of those is biomarker discovery and diagnostics. Key sample preparation enabled a greater range clinical samples be characterized at deeper level using MS. While most these focused on blood, tissues also been an important resource. Fresh tissues, however, are difficult obtain research purposes require significant resources long-term storage. There millions archived formalin-fixed paraffin-embedded (FFPE) within pathology departments worldwide representing every possible tissue type including tumors that rare or very small. Owing the chemical technique used preserve FFPE they were considered intractable many newer proteomics techniques primarily only useful immunohistochemistry. In past couple decades, researchers able develop methods extract proteins from form making them analyzable state-of-the-art technologies such as MS protein arrays. This review will discuss history provide examples how currently being identify biomarkers diagnose diseases cancer.

Language: Английский

Citations

7
Changes of PK/PD of Meropenem in patients with abdominal septic shock and exploration of clinical rational administration plan: a prospective exploratory study DOI Creative Commons
Youquan Wang, Hongxiang Li, Dongxia Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 3, 2024

Abstract This study aimed to explore the changes of pharmacokinetic parameters after meropenem in patients with abdominal septic shock gastrointestinal perforation, and simulate probability different dosing regimens achieving pharmacodynamic goals. The included 12 patients, utilized high performance liquid chromatography-tandem mass spectrometry monitor plasma concentration meropenem. target attainment (PTA) for minimum inhibitory (MIC) values %fT > 4MIC was compared among simulated regimens. results showed that 96 blood samples from clearance (CL) normal abnormal creatinine subgroups were 7.7 ± 1.8 4.4 1.1 L/h, respectively, apparent volume distribution (Vd) 22.6 5.1 17.2 5.8 L, respectively. 2. Regardless subgroup, 0.5 g/q6h infusion over 6 h regimen achieved a PTA 90% when MIC ≤ mg/L. 1.0 other regimen, most cases, making is higher. For at low MIC, may be preferable. dose g/q6 Further research needed confirm this exploratory result.

Language: Английский

Citations

2
Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology DOI Creative Commons
Paul Dowling, Dieter Swandulla, Kay Ohlendieck

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(21), P. 2560 - 2560

Published: Nov. 1, 2023

Voluntary striated muscles are characterized by a highly complex and dynamic proteome that efficiently adapts to changed physiological demands or alters considerably during pathophysiological dysfunction. The skeletal muscle has been extensively studied in relation myogenesis, fiber type specification, transitions, the effects of physical exercise, disuse atrophy, neuromuscular disorders, co-morbidities sarcopenia old age. Since tissue accounts for approximately 40% body mass humans, alterations have considerable influence on whole-body physiology. This review outlines main bioanalytical avenues taken proteomic characterization tissues, including top-down proteomics focusing intact proteoforms their post-translational modifications, bottom-up proteomics, which is peptide-centric method concerned with large-scale detection proteins mixtures, subproteomics examines protein composition distinct subcellular fractions. Mass spectrometric studies over last two decades decisively improved our general cell biological understanding diversity heterogeneous individual myofibers muscles. detailed knowledge can now be integrated findings from other omics-type methodologies establish systems view function.

Language: Английский

Citations

6