Aquatic Toxicology, Год журнала: 2024, Номер unknown, С. 107110 - 107110
Опубликована: Сен. 1, 2024
Язык: Английский
Nucleic Acids Research, Год журнала: 2018, Номер 47(D1), С. D812 - D820
Опубликована: Ноя. 26, 2018
Flatworms (Platyhelminthes) are a basally branching phylum that harbours wealth of fascinating biology, including planarians with their astonishing regenerative abilities and the parasitic tape worms blood flukes exert massive impact on human health. PlanMine (http://planmine.mpi-cbg.de/) has mission objective providing both mineable sequence repository for also resource comparative analysis flatworm biology. While original release was entirely based transcriptomes, current transitions to more genomic perspective. Building recent availability high quality genome assembly planarian model species Schmidtea mediterranea, we provide gene prediction set now assign existing transcripts defined coordinates. The addition single cell bulk RNA-seq datasets greatly expands available expression information. Further, add transcriptomes from broad range other flatworms phylogeny-aware interface makes evolutionary comparisons accessible non-experts. At its core, continues utilize powerful InterMine framework consistent data annotations enable meaningful inter-species comparisons. Overall, 3.0 thus provides host new features biology wider research community.
Язык: Английский
Процитировано
158
Development, Год журнала: 2019, Номер 146(17)
Опубликована: Сен. 1, 2019
ABSTRACT Planarians are a group of flatworms. Some planarian species have remarkable regenerative abilities, which involve abundant pluripotent adult stem cells. This makes these worms powerful model system for understanding the molecular and evolutionary underpinnings regeneration. By providing succinct overview taxonomy, anatomy, available tools orchestration regeneration, this Primer aims to showcase both unique assets questions that can be addressed with system.
Язык: Английский
Процитировано
114
Nature Ecology & Evolution, Год журнала: 2023, Номер 7(12), С. 2108 - 2124
Опубликована: Окт. 19, 2023
Regenerative abilities vary dramatically across animals. Even amongst planarian flatworms, well-known for complete regeneration from tiny body fragments, some species have restricted while others are almost entirely incompetent. Here, we assemble a diverse live collection of 40 to probe the evolution head in group. Combining quantification species-specific head-regeneration with comprehensive transcriptome-based phylogeny reconstruction, show multiple independent transitions between robust whole-body and freshwater species. RNA-mediated genetic interference inhibition canonical Wnt signalling interference-sensitive bypassed all defects, suggesting that pathway is linked emergence defects. Our finding has roles reproductive system model Schmidtea mediterranea raises possibility trade-off egg-laying, asexual reproduction by fission/regeneration drives regenerative trait evolution. Although quantitative comparisons levels, yolk content strategy our remained inconclusive, they revealed divergent planarians. Altogether, study establishes planarians as taxon comparative research presents framework mechanistic abilities.
Язык: Английский
Процитировано
29
Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2023, Номер 99(1), С. 131 - 176
Опубликована: Сен. 12, 2023
ABSTRACT Aquatic invertebrates play a pivotal role in (eco)toxicological assessments because they offer ethical, cost‐effective and repeatable testing options. Additionally, their significance the food chain ability to represent diverse aquatic ecosystems make them valuable subjects for studies. To ensure consistency comparability across studies, international (eco)toxicology guidelines have been used establish standardised methods protocols data collection, analysis interpretation. However, current primarily focus on limited number of invertebrate species, mainly from Arthropoda, Mollusca Annelida. These are suitable basic toxicity screening, effectively assessing immediate severe effects toxic substances organisms. For more comprehensive ecologically relevant assessments, particularly those addressing long‐term ecosystem‐wide impacts, we recommended use broader diversity since present choice taxa exacerbates scope ecotoxicological This review provides overview focusing major how assess impact chemicals environments. The work supports broad‐taxa approach environmental as it better represents natural populations inhabiting various ecosystems. Advances omics other biochemical computational techniques feasible, enabling mechanistic studies non‐model By combining these approaches with vitro together approach, researchers can gain insights into less‐explored impacts pollution, such changes population diversity, development tolerance transgenerational inheritance pollution responses, organism phenotypic plasticity, biological invasion outcomes, social behaviour changes, metabolome regeneration phenomena, disease susceptibility tissue pathologies. also emphasises need harmonised data‐reporting standards minimum annotation checklists that research results findable, accessible, interoperable reusable (FAIR), maximising reusability data. ultimate goal is encourage integrated holistic problem‐focused collaboration between scientific disciplines, standardisation organisations decision‐making bodies, transdisciplinary knowledge co‐production One‐Health approach.
Язык: Английский
Процитировано
19
Опубликована: Сен. 30, 2019
Reproduction is a fundamental feature of life, it the way life persists across ages. This book offers new, wider vistas on this biological phenomenon, exploring how works through whole tree life. It explores facets such as asexual reproduction, parthenogenesis, sex determination and reproductive investment, with taxonomic coverage extended over all main groups - animals, plants including 'algae', fungi, protists bacteria. collates into one volume perspectives from varied disciplines zoology, botany, microbiology, genetics, cell biology, developmental evolutionary animal plant physiology, ethology integrating information common language. Crucially, aims to identify commonalties among phenomena, while demonstrating diversity even amongst closely related taxa. Its integrated approach makes valuable reference for students researchers, well an effective entry point deeper study specific topics.
Язык: Английский
Процитировано
53
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 5, 2025
Abstract To fully comprehend host-microorganism interactions, it is crucial to understand the composition and diversity of microbiome, as well factors that shape these characteristics. We investigated microbiome variation using freshwater planarian Schmidtea mediterranea , an invertebrate model in regeneration biology (eco-)toxicology, by exposing organisms various controlled conditions. The exhibited high variability, with most bacteria belonging Betaproteobacteria. Among diverse microbial communities, a few genera, such Curvibacter were consistently present, but significant alterations response changing relative abundance fluctuated during process, initially increasing before returning similar beginning situation. After applying external stress, other genera decreased. Variation over time, between different origin laboratories individuals, showed additional, yet to-be-identified, are present. Taking all results together, our study provides solid basis for future research focusing on bacterial functionality planarians invertebrates.
Язык: Английский
Процитировано
0
Environmental Pollution, Год журнала: 2020, Номер 266, С. 115185 - 115185
Опубликована: Июль 9, 2020
Язык: Английский
Процитировано
34
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Дек. 23, 2023
Abstract The planarian Schmidtea mediterranea can regenerate its entire body from small tissue fragments and is studied as regeneration model species. assembly functional analysis of genomes has proven challenging due high A/T content (70% A/T), repetitive nature, limited transferability routine laboratory protocols to their divergent biochemistry. Only few often fragmented genome assemblies are currently available, open challenges include the provision well-annotated chromosome-scale reference species other planarians for a comparative evolution perspective. Here we report haplotype-phased, high-quality gene annotations sexual S2 strain S. provide putative regulatory region via optimized ATAC-seq ChIP-seq protocols. To additionally leverage sequence conservation element annotations, generated chromatin accessibility data three closest relatives : polychroa , nova lugubris . We find substantial divergence in protein-coding sequences regions, yet reveal remarkable ChIP-mark bearing regions identified promoters enhancers resulting high-confidence set evolutionary conserved provides valuable resource circuits within taxon. In addition, our four first perspective on evolution. Our analyses frequent retrotransposon-associated chromosomal inversions inter-chromosomal translocations that lead degradation synteny across genus. Interestingly, further independent near-complete losses ancestral metazoan two flatworm groups, indicating platyhelminth largely evolve without syntenic constraints. work resources research community sets foundation genomics planarians. contrast between fast structural elements, suggesting unique flatworms where positioning may not be essential.
Язык: Английский
Процитировано
12
Cell Reports Methods, Год журнала: 2022, Номер 2(10), С. 100298 - 100298
Опубликована: Сен. 20, 2022
Planarians have long been studied for their regenerative abilities. Moving forward, tools ectopic expression of non-native proteins will be substantial value. Using a luminescent reporter to overcome the strong autofluorescence planarian tissues, we demonstrate heterologous protein in cells and live animals. Our approach is based on introduction mRNA through several nanotechnological chemical transfection methods. We improve by altering untranslated region (UTR) sequences codon bias, facilitating measurement kinetics both isolated whole planarians using luminescence imaging
Язык: Английский
Процитировано
16
Biological Journal of the Linnean Society, Год журнала: 2023, Номер 140(3), С. 423 - 433
Опубликована: Авг. 17, 2023
Abstract Cuban biodiversity is characterized by high species richness and endemism; however, free-living flatworms have been neglected in studies of the fauna archipelago. These animals constitute an essential component marine freshwater ecosystems as top predators secondary producers. In this contribution, we provide first comprehensive analysis turbellarian diversity Cuba based on a long-term sampling effort marine, brackish, terrestrial environments. We used observed estimated indicators alpha diversity. As result, collected, for time, 279 Cuba, including 189 rhabdocoels, 33 polyclads, 21 macrostomorphs, 14 proseriates, 12 prolecithophorans, seven triclads one representative each Prorhynchida, Gnosonesimida Bothrioplanida. At least 184 (67%) are new to science. Fifty these recorded published journal contributions. The remainder pending formal identification and/or description. demonstrate be endemism. Estimated much higher than that observed, exemplifying taxonomic impediments stressing need more intense campaigns
Язык: Английский
Процитировано
9