Targeted genome-modification tools and their advanced applications in crop breeding

Nature Reviews Genetics, Год журнала: 2024, Номер 25(9), С. 603 - 622

Опубликована: Апрель 24, 2024

Язык: Английский

---

SYNTHETIC BIOLOGY AND ITS POTENTIAL IN U.S. MEDICAL THERAPEUTICS: A COMPREHENSIVE REVIEW: EXPLORING THE CUTTING-EDGE INTERSECTIONS OF BIOLOGY AND ENGINEERING IN DRUG DEVELOPMENT AND TREATMENTS

Engineering Science & Technology Journal, Год журнала: 2024, Номер 5(4), С. 1395 - 1414

Опубликована: Апрель 17, 2024

This study provides a comprehensive review of the cutting-edge intersections biology and engineering in realm medical therapeutics, focusing on advancements applications synthetic biology. The main objectives were to explore historical evolution, define scope, analyze current state-of-the-art biology, particularly drug development treatment strategies. methodology employed systematic literature content analysis, utilizing range academic databases gather relevant peer-reviewed articles published between 2013 2023. Key findings revealED that through integration principles biological systems, has led significant innovations therapeutics. These include novel delivery targeted therapies, personalized medicine approaches. highlights challenges limitations approaches, such as ethical concerns, regulatory hurdles, need for interdisciplinary collaboration. future landscape is marked by both prospects. field holds immense potential addressing complex diseases enhancing healthcare delivery, yet faces considerations frameworks. Strategic recommendations are provided researchers, industry leaders, policymakers, emphasizing continuous innovation, applications, Finally, stands transformative force with revolutionize healthcare. Future research should focus overcoming exploring new ensuring field's continued advancement contribution global healthcare. Keywords: Synthetic Biology, Medical Therapeutics, Drug Development, Bioengineering.

Язык: Английский

---

The genomic route to tomato breeding: Past, present, and future

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 195(4), С. 2500 - 2514

Опубликована: Апрель 30, 2024

Over the past 10,000 years, tomato species have undergone both unintentional and intentional selection to enhance their favorable traits for human consumption manufacturing. These processes significantly influenced genomes of played a critical role in improving varieties. In this review, we summarize recent advances genome sequencing, explore impact human-driven selection, recapitulate key genes associated with important agronomic breeding. We provide several examples genomics-guided breeding highlight potential resources facilitating improvement. Furthermore, elaborate progress strategies through design present how such efforts can help future enhancements align demands sustainability evolving societies.

Язык: Английский

---

Exploiting synthetic biology platforms for enhanced biosynthesis of natural products in Yarrowia lipolytica

Bioresource Technology, Год журнала: 2024, Номер 399, С. 130614 - 130614

Опубликована: Март 19, 2024

Язык: Английский

---

Efficient data reconstruction: The bottleneck of large-scale application of DNA storage

Cell Reports, Год журнала: 2024, Номер 43(4), С. 113699 - 113699

Опубликована: Март 21, 2024

Over the past decade, rapid development of DNA synthesis and sequencing technologies has enabled preliminary use molecules for digital data storage, overcoming capacity persistence bottlenecks silicon-based storage media. now been fully accomplished in laboratory through existing biotechnology, which again demonstrates viability carbon-based However, high cost latency reconstruction pose challenges that hinder practical implementation beyond laboratory. In this article, we review advanced methods, analyze characteristics performance biotechnological approaches at various stages writing reading, discuss potential factors influencing from perspective reconstruction.

Язык: Английский

---

Enhancing glucaric acid production from myo -inositol in Escherichia coli by eliminating cell-to-cell variation

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(6)

Опубликована: Май 29, 2024

Glucaric acid (GA) is a value-added chemical and can be used to manufacture food additives, anticancer drugs, polymers. The non-genetic cell-to-cell variations in GA biosynthesis are naturally inherent, indicating the presence of both high- low-performance cells culture. Low-performance lead nutrient waste inefficient production. Furthermore,

Язык: Английский

---

Building a eukaryotic chromosome arm by de novo design and synthesis

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 30, 2023

The genome of an organism is inherited from its ancestor and continues to evolve over time, however, the extent which current version could be altered remains unknown. To probe plasticity Saccharomyces cerevisiae, here we replace native left arm chromosome XII (chrXIIL) with a linear artificial harboring small sets reconstructed genes. We find that as few 12 genes are sufficient for cell viability, whereas 25 required recover partial fitness defects observed in 12-gene strain. Next, demonstrate these can individually using synthetic regulatory sequences recoded open-reading frames "one-amino-acid-one-codon" strategy remain functional. Finally, neochromsome assembled substitute chrXIIL viability. Together, our work not only highlights high yeast genome, but also illustrates possibility making functional eukaryotic chromosomes entirely sequences.

Язык: Английский

---

High plasticity of ribosomal DNA organization in budding yeast

Cell Reports, Год журнала: 2024, Номер 43(2), С. 113742 - 113742

Опубликована: Фев. 1, 2024

In eukaryotic genomes, rDNA generally resides as a highly repetitive and dynamic structure, making it difficult to study. Here, synthetic array on chromosome III in budding yeast was constructed serve the sole source of rRNA. Utilizing loxPsym site within each repeat Cre recombinase, we were able reduce copy number few eight copies. Additionally, strains with two or three arrays found that presence multiple did not affect formation single nucleolus. Although alteration position impact three-dimensional genome additional had no deleterious influence cell growth transcriptomes. Overall, this study sheds light high plasticity organization opens up opportunities for future engineering.

Язык: Английский

---

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(8), С. 4301 - 4316

Опубликована: Фев. 12, 2024

This study optimized the menaquinone-7 (MK-7) synthetic pathways in Bacillus subtilis (B. subtilis) natto NB205, a strain that originated from natto, to enhance its MK-7 production. Utilizing mutation breeding, we developed NBMK308, mutant demonstrated significant 117.23% increase A comprehensive transcriptome analysis identified two key genes, ispA and ispE, as being critical synthesis. The dual-sgRNA CRISPRa system was utilized achieve precise regulation of ispE newly engineered strain, A3E3. strategic modulation resulted enhancement production, achieving increases 20.02% 201.41% compared traditional overexpression systems original respectively. Furthermore, fermentation supernatant A3E3 notably inhibited Salmonella invasion Caco-2 cells, showcasing potential for combating such infections. safety confirmed through cell assays. utilization this crucial genes synthesis, leading remarkable production demonstrating additional therapeutic inhibiting pathogenic approach effectively combined advantages microbial biotechnology, addressing health nutritional challenges.

Язык: Английский

---

纳米材料助力合成生物学的生物医学应用

Science China Materials, Год журнала: 2024, Номер 67(7), С. 2051 - 2066

Опубликована: Май 22, 2024

Язык: Английский

---