Advanced Materials,
Год журнала:
2023,
Номер
36(10)
Опубликована: Фев. 25, 2023
Nanomaterials
with
more
than
one
enzyme-like
activity
are
termed
multienzymic
nanozymes,
and
they
have
received
increasing
attention
in
recent
years
hold
huge
potential
to
be
applied
diverse
fields,
especially
for
biosensing
therapeutics.
Compared
single
nanozymes
offer
various
unique
advantages,
including
synergistic
effects,
cascaded
reactions,
environmentally
responsive
selectivity.
Nevertheless,
along
these
merits,
the
catalytic
mechanism
rational
design
of
complicated
elusive
as
compared
single-enzymic
nanozymes.
In
this
review,
classification
scheme
based
on
numbers/types
activities,
internal
external
factors
regulating
multienzymatic
chemical,
biomimetic,
computer-aided
strategies,
progress
applications
attributed
advantages
multicatalytic
activities
systematically
discussed.
Finally,
current
challenges
future
perspectives
regarding
development
application
suggested.
This
review
aims
deepen
understanding
inspire
research
a
greater
extent.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(17), С. 9488 - 9507
Опубликована: Март 31, 2023
Arming
activatable
mild-photothermal
therapy
(PTT)
with
the
property
of
relieving
tumor
thermotolerance
holds
great
promise
for
overcoming
traditional
mild
PTT
limitations
such
as
thermoresistance,
insufficient
therapeutic
effect,
and
off-target
heating.
Herein,
a
mitochondria-targeting,
defect-engineered
AFCT
nanozyme
enhanced
multi-enzymatic
activity
was
elaborately
designed
microenvironment
(TME)-activatable
phototheranostic
agent
to
achieve
remarkable
anti-tumor
via
"electron
transport
chain
(ETC)
interference
synergistic
adjuvant
therapy".
Density
functional
theory
calculations
revealed
that
effect
among
multi-enzyme
active
centers
endows
nanozymes
excellent
catalytic
activity.
In
TME,
open
sources
H2O2
can
be
achieved
by
superoxide
dismutase-mimicking
nanozymes.
response
dual
stimuli
acidity,
peroxidase-mimicking
not
only
catalyzes
accumulation
generate
·OH
but
also
converts
loaded
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid)
(ABTS)
into
its
oxidized
form
strong
near-infrared
absorption,
specifically
unlocking
photothermal
photoacoustic
imaging
properties.
Intriguingly,
undesired
thermoresistance
cells
greatly
alleviated
owing
reduced
expression
heat
shock
proteins
enabled
NADH
POD-mimicking
AFCT-mediated
depletion
consequent
restriction
ATP
supply.
Meanwhile,
accumulated
facilitate
both
apoptosis
ferroptosis
in
cells,
resulting
outcomes
combination
TME-activated
PTT.
ACS Nano,
Год журнала:
2022,
Номер
16(9), С. 15397 - 15412
Опубликована: Сен. 15, 2022
An
elevated
bone
microenvironmental
reactive
oxygen
species
(ROS)
level
is
a
hallmark
of
osteoporosis
that
often
leads
to
the
dysfunction
bone-related
mesenchymal
stem
cells
(MSCs),
which
would
induce
MSC
senescence
and
severely
undermine
their
osteoblastic
potential.
Herein,
we
report
in
situ
construction
microenvironment-responsive
biofunctional
metal-organic
framework
(bio-MOF)
coating
on
titanium
surface
through
coordination
between
p-xylylenebisphosphonate
(PXBP)
Ce/Sr
ions
by
hydrothermal
method.
Taking
advantage
anchored
Ce
Sr
ions,
AHT-Ce/SrMOF
implants
demonstrate
on-demand
superoxide
dismutase
catalase-like
catalytic
activities
decompose
ROS
MSCs
restore
mitochondrial
functions.
In
vitro
analysis
showed
substantially
activated
AMP-activated
protein
kinase
(AMPK)
signaling
pathway
reduced
levels.
Meanwhile,
grown
displayed
significantly
higher
expressions
fission
marker
(DRP1),
fusion
(MFN2
OPA1),
mitophagy
(PINK1
LC3)
than
those
AHT-CeMOF
AHT-SrMOF
groups,
indicated
bio-MOF
could
amend
function
reverse
senescence.
vivo
evaluations
bio-MOF-coated
Ti
implant
site
promote
new
formation,
leading
improved
osteointegration
osteoporotic
rat.
This
study
may
improve
implant-mediated
fracture
healing
clinics.
ACS Nano,
Год журнала:
2023,
Номер
17(17), С. 17383 - 17393
Опубликована: Авг. 14, 2023
Nanozymes
are
promising
alternatives
to
natural
enzymes,
but
their
use
remains
limited
owing
poor
specificity.
For
example,
CeO2
activates
H2O2
and
displays
peroxidase
(POD)-like,
catalase
(CAT)-like,
haloperoxidase
(HPO)-like
activities.
Since
they
unavoidably
compete
for
H2O2,
affecting
its
utilization
in
the
target
application,
precise
manipulation
of
reaction
specificity
is
thus
imperative.
Herein,
we
showed
that
one
can
simply
achieve
this
by
manipulating
activation
pathway
on
pristine
well-defined
shapes.
This
because
coordination
electronic
structures
Ce
sites
vary
with
surfaces,
wherein
(100)
(111)
surfaces
display
nearly
100%
toward
POD-/CAT-like
HPO-like
activities,
respectively.
The
antibacterial
results
suggest
latter
surface
well-utilize
kill
bacteria
(cf.,
former),
which
anti-biofouling
applications.
work
provides
atomic
insights
into
synthesis
nanozymes
improved
activity,
specificity,
utilization.
Abstract
On
accounts
of
the
advantages
inherent
high
stability,
ease
preparation
and
superior
catalytic
activities,
nanozymes
have
attracted
tremendous
potential
in
diverse
biomedical
applications
as
alternatives
to
natural
enzymes.
Optimizing
activity
is
significant
for
widening
boosting
into
practical
level.
As
research
regulation
strategies
boosting,
it
essential
timely
review,
summarize,
analyze
advances
structure–activity
relationships
further
inspiring
ingenious
this
prosperous
area.
Herein,
methods
recent
5
years
are
systematically
summarized,
including
size
morphology,
doping,
vacancy,
surface
modification,
hybridization,
followed
by
a
discussion
latest
consisting
biosensing,
antibacterial,
tumor
therapy.
Finally,
challenges
opportunities
rapidly
developing
field
presented
more
infant
yet
promising
Cellular and Molecular Life Sciences,
Год журнала:
2023,
Номер
80(2)
Опубликована: Янв. 19, 2023
Abstract
Nanoceria
or
cerium
oxide
nanoparticles
characterised
by
the
co-existing
of
Ce
3+
and
4+
that
allows
self-regenerative,
redox-responsive
dual-catalytic
activities,
have
attracted
interest
as
an
innovative
approach
to
treating
cancer.
Depending
on
surface
characteristics
immediate
environment,
nanoceria
exerts
either
anti-
pro-oxidative
effects
which
regulate
reactive
oxygen
species
(ROS)
levels
in
biological
systems.
mimics
ROS-related
enzymes
protect
normal
cells
at
physiological
pH
from
oxidative
stress
induce
ROS
production
slightly
acidic
tumour
microenvironment
trigger
cancer
cell
death.
nanozymes
also
generates
molecular
relieves
hypoxia,
leading
sensitisation
improve
therapeutic
outcomes
photodynamic
(PDT),
photothermal
(PTT)
radiation
(RT),
targeted
chemotherapies.
has
been
engineered
a
nanocarrier
drug
delivery
combination
with
other
drugs
produce
synergistic
anti-cancer
effects.
Despite
reported
preclinical
successes,
there
are
still
knowledge
gaps
arising
inadequate
number
studies
reporting
findings
based
physiologically
relevant
disease
models
accurately
represent
complexities
This
review
discusses
activities
responding
tension
gradient
microenvironment,
highlights
recent
nanoceria-based
platforms
be
feasible
direct
indirect
agents
protective
healthy
tissues,
finally
addresses
challenges
clinical
translation
therapeutics.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
12(31)
Опубликована: Сен. 14, 2023
Abstract
Although
CeO
2
nanomaterials
have
been
widely
explored
as
nanozymes
for
catalytic
therapy,
they
still
suffer
from
relatively
low
activities.
Herein,
the
catalyzing
generation
and
stabilization
of
oxygen
vacancies
on
nanorods
by
Pt
nanoclusters
via
H
gas
reduction
under
mild
temperature
(350
°C)
to
obtain
Pt/CeO
2−
x
,
which
can
serve
a
highly
efficient
nanozyme
cancer
is
reported.
The
deposited
atomic
layer
deposition
technique
not
only
catalyst
generate
through
hydrogen
spillover
effect,
but
also
stabilize
generated
vacancies.
Meanwhile,
provide
anchoring
sites
forming
strong
metal‐support
interactions
thus
preventing
their
agglomerations.
Importantly,
reduced
at
350
°C
(Pt/CeO
‐350R)
exhibits
excellent
enzyme‐mimicking
activity
reactive
species
(e.g.,
·OH)
compared
other
control
samples,
including
temperatures,
achieving
performance
tumor‐specific
therapy
efficiently
eliminate
cells
in
vitro
ablate
tumors
vivo.
‐350R
originates
good
activities
vacancy‐rich
nanoclusters.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Фев. 3, 2024
Abstract
The
reaction
system
of
hydrogen
peroxide
(H
2
O
)
catalyzed
by
nanozyme
has
a
broad
prospect
in
antibacterial
treatment.
However,
the
complex
catalytic
activities
nanozymes
lead
to
multiple
pathways
reacting
parallel,
causing
uncertain
results.
New
approach
effectively
regulate
is
urgent
need.
Herein,
Cu
single
site
modified
on
nanoceria
with
various
activities,
such
as
peroxidase-like
activity
(POD)
and
hydroxyl
radical
antioxidant
capacity
(HORAC).
Benefiting
from
interaction
between
coordinated
CeO
substrate,
POD
enhanced
while
HORAC
inhibited,
which
further
confirmed
density
functional
theory
(DFT)
calculations.
Cu-CeO
+
H
shows
good
properties
both
vitro
vivo.
In
this
work,
strategy
based
metal
carrier
provides
general
clue
for
optimizing
nanozymes.
