Iron chelators loaded on myocardiocyte mitochondria-targeted nanozyme system for treating myocardial ischemia-reperfusion injury in mouse models DOI Creative Commons
Ke Zhu, Kun Wang,

Rongting Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 15, 2025

Ferroptosis plays a critical role in myocardial ischemia-reperfusion injury (MIRI), posing significant clinical challenge. Nanoenzymes like cerium oxide (CeO2) hold promise for mitigating oxidative damage and inhibiting ferroptosis, but their delivery efficiency biological activity require optimization. This study aims to develop targeted nanozyme system MIRI treatment by integrating CeO2 with mesoporous polydopamine (mPDA) dexrazoxane (DXZ) achieve synergistic therapeutic effects. A biomineralization technique was used synthesize nanoparticles (2–3 nm) within mPDA, forming ~ 130 nm composite (Ce@mPDA). Surface modifications cardiac homing peptide (CHP) triphenylphosphine (TPP) enabled hierarchical targeting injured myocardium mitochondria. DXZ-loaded Ce@mPDA-C/P (D/Ce@mPDA-C/P) were evaluated vitro mouse model effects on stress, apoptosis, inflammation, function. D/Ce@mPDA-C/P exhibited robust ROS scavenging, sustained DXZ release, efficient mitochondrial targeting. The significantly reduced upregulated GPX4 expression, inhibited modulated the inflammatory microenvironment. Long-term studies demonstrated reductions fibrosis improvements function, including enhanced fractional shortening ejection fraction. effectively combines antioxidant properties of iron-chelating DXZ, providing promising strategy MIRI. approach may expand use advance nanomedicine-based interventions repair.

Language: Английский

Multienzyme‐Like Nanozymes: Regulation, Rational Design, and Application DOI
Jingyi Sheng,

Yuehuang Wu,

He Ding

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: Feb. 25, 2023

Nanomaterials with more than one enzyme-like activity are termed multienzymic nanozymes, and they have received increasing attention in recent years hold huge potential to be applied diverse fields, especially for biosensing therapeutics. Compared single nanozymes offer various unique advantages, including synergistic effects, cascaded reactions, environmentally responsive selectivity. Nevertheless, along these merits, the catalytic mechanism rational design of complicated elusive as compared single-enzymic nanozymes. In this review, classification scheme based on numbers/types activities, internal external factors regulating multienzymatic chemical, biomimetic, computer-aided strategies, progress applications attributed advantages multicatalytic activities systematically discussed. Finally, current challenges future perspectives regarding development application suggested. This review aims deepen understanding inspire research a greater extent.

Language: Английский

Citations

202
“Electron Transport Chain Interference” Strategy of Amplified Mild-Photothermal Therapy and Defect-Engineered Multi-Enzymatic Activities for Synergistic Tumor-Personalized Suppression DOI
Shuming Dong, Yushan Dong, Zhiyu Zhao

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(17), P. 9488 - 9507

Published: March 31, 2023

Arming activatable mild-photothermal therapy (PTT) with the property of relieving tumor thermotolerance holds great promise for overcoming traditional mild PTT limitations such as thermoresistance, insufficient therapeutic effect, and off-target heating. Herein, a mitochondria-targeting, defect-engineered AFCT nanozyme enhanced multi-enzymatic activity was elaborately designed microenvironment (TME)-activatable phototheranostic agent to achieve remarkable anti-tumor via "electron transport chain (ETC) interference synergistic adjuvant therapy". Density functional theory calculations revealed that effect among multi-enzyme active centers endows nanozymes excellent catalytic activity. In TME, open sources H2O2 can be achieved by superoxide dismutase-mimicking nanozymes. response dual stimuli acidity, peroxidase-mimicking not only catalyzes accumulation generate ·OH but also converts loaded 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) into its oxidized form strong near-infrared absorption, specifically unlocking photothermal photoacoustic imaging properties. Intriguingly, undesired thermoresistance cells greatly alleviated owing reduced expression heat shock proteins enabled NADH POD-mimicking AFCT-mediated depletion consequent restriction ATP supply. Meanwhile, accumulated facilitate both apoptosis ferroptosis in cells, resulting outcomes combination TME-activated PTT.

Language: Английский

Citations

170
Nanozyme-based biosensor for organophosphorus pesticide monitoring: Functional design, biosensing strategy, and detection application DOI
Fengnian Zhao, Li Wang, Mengyue Li

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 165, P. 117152 - 117152

Published: June 20, 2023

Language: Английский

Citations

78
Nanocatalytic Biofunctional MOF Coating on Titanium Implants Promotes Osteoporotic Bone Regeneration through Cooperative Pro-osteoblastogenesis MSC Reprogramming DOI
Maowen Chen, Dong Wang,

Menghuan Li

et al.

ACS Nano, Journal Year: 2022, Volume and Issue: 16(9), P. 15397 - 15412

Published: Sept. 15, 2022

An elevated bone microenvironmental reactive oxygen species (ROS) level is a hallmark of osteoporosis that often leads to the dysfunction bone-related mesenchymal stem cells (MSCs), which would induce MSC senescence and severely undermine their osteoblastic potential. Herein, we report in situ construction microenvironment-responsive biofunctional metal-organic framework (bio-MOF) coating on titanium surface through coordination between p-xylylenebisphosphonate (PXBP) Ce/Sr ions by hydrothermal method. Taking advantage anchored Ce Sr ions, AHT-Ce/SrMOF implants demonstrate on-demand superoxide dismutase catalase-like catalytic activities decompose ROS MSCs restore mitochondrial functions. In vitro analysis showed substantially activated AMP-activated protein kinase (AMPK) signaling pathway reduced levels. Meanwhile, grown displayed significantly higher expressions fission marker (DRP1), fusion (MFN2 OPA1), mitophagy (PINK1 LC3) than those AHT-CeMOF AHT-SrMOF groups, indicated bio-MOF could amend function reverse senescence. vivo evaluations bio-MOF-coated Ti implant site promote new formation, leading improved osteointegration osteoporotic rat. This study may improve implant-mediated fracture healing clinics.

Language: Английский

Citations

75
Regulating the H2O2 Activation Pathway on a Well-Defined CeO2 Nanozyme Allows the Entire Steering of Its Specificity between Associated Enzymatic Reactions DOI
Bo Yuan, Zicong Tan, Qiang Guo

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(17), P. 17383 - 17393

Published: Aug. 14, 2023

Nanozymes are promising alternatives to natural enzymes, but their use remains limited owing poor specificity. For example, CeO2 activates H2O2 and displays peroxidase (POD)-like, catalase (CAT)-like, haloperoxidase (HPO)-like activities. Since they unavoidably compete for H2O2, affecting its utilization in the target application, precise manipulation of reaction specificity is thus imperative. Herein, we showed that one can simply achieve this by manipulating activation pathway on pristine well-defined shapes. This because coordination electronic structures Ce sites vary with surfaces, wherein (100) (111) surfaces display nearly 100% toward POD-/CAT-like HPO-like activities, respectively. The antibacterial results suggest latter surface well-utilize kill bacteria (cf., former), which anti-biofouling applications. work provides atomic insights into synthesis nanozymes improved activity, specificity, utilization.

Language: Английский

Citations

64
Activity Regulating Strategies of Nanozymes for Biomedical Applications DOI
Xiaoteng Ding, Zhen Zhao, Yanfang Zhang

et al.

Small, Journal Year: 2023, Volume and Issue: 19(11)

Published: Jan. 17, 2023

Abstract On accounts of the advantages inherent high stability, ease preparation and superior catalytic activities, nanozymes have attracted tremendous potential in diverse biomedical applications as alternatives to natural enzymes. Optimizing activity is significant for widening boosting into practical level. As research regulation strategies boosting, it essential timely review, summarize, analyze advances structure–activity relationships further inspiring ingenious this prosperous area. Herein, methods recent 5 years are systematically summarized, including size morphology, doping, vacancy, surface modification, hybridization, followed by a discussion latest consisting biosensing, antibacterial, tumor therapy. Finally, challenges opportunities rapidly developing field presented more infant yet promising

Language: Английский

Citations

55
Nanoceria: an innovative strategy for cancer treatment DOI Creative Commons
Joyce Tang, Shehzahdi S. Moonshi, Hang T. Ta

et al.

Cellular and Molecular Life Sciences, Journal Year: 2023, Volume and Issue: 80(2)

Published: Jan. 19, 2023

Abstract Nanoceria or cerium oxide nanoparticles characterised by the co-existing of Ce 3+ and 4+ that allows self-regenerative, redox-responsive dual-catalytic activities, have attracted interest as an innovative approach to treating cancer. Depending on surface characteristics immediate environment, nanoceria exerts either anti- pro-oxidative effects which regulate reactive oxygen species (ROS) levels in biological systems. mimics ROS-related enzymes protect normal cells at physiological pH from oxidative stress induce ROS production slightly acidic tumour microenvironment trigger cancer cell death. nanozymes also generates molecular relieves hypoxia, leading sensitisation improve therapeutic outcomes photodynamic (PDT), photothermal (PTT) radiation (RT), targeted chemotherapies. has been engineered a nanocarrier drug delivery combination with other drugs produce synergistic anti-cancer effects. Despite reported preclinical successes, there are still knowledge gaps arising inadequate number studies reporting findings based physiologically relevant disease models accurately represent complexities This review discusses activities responding tension gradient microenvironment, highlights recent nanoceria-based platforms be feasible direct indirect agents protective healthy tissues, finally addresses challenges clinical translation therapeutics.

Language: Английский

Citations

48
Molecular insights of nanozymes from design to catalytic mechanism DOI Open Access
Yuan Xu, Zhixin Zhou,

Nankai Deng

et al.

Science China Chemistry, Journal Year: 2023, Volume and Issue: 66(5), P. 1318 - 1335

Published: Feb. 9, 2023

Language: Английский

Citations

47
Catalyzing Generation and Stabilization of Oxygen Vacancies on CeO2−x Nanorods by Pt Nanoclusters as Nanozymes for Catalytic Therapy DOI Creative Commons
Jiankang Zhang, Yu Yang,

Fengmin Qin

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(31)

Published: Sept. 14, 2023

Abstract Although CeO 2 nanomaterials have been widely explored as nanozymes for catalytic therapy, they still suffer from relatively low activities. Herein, the catalyzing generation and stabilization of oxygen vacancies on nanorods by Pt nanoclusters via H gas reduction under mild temperature (350 °C) to obtain Pt/CeO 2− x , which can serve a highly efficient nanozyme cancer is reported. The deposited atomic layer deposition technique not only catalyst generate through hydrogen spillover effect, but also stabilize generated vacancies. Meanwhile, provide anchoring sites forming strong metal‐support interactions thus preventing their agglomerations. Importantly, reduced at 350 °C (Pt/CeO ‐350R) exhibits excellent enzyme‐mimicking activity reactive species (e.g., ·OH) compared other control samples, including temperatures, achieving performance tumor‐specific therapy efficiently eliminate cells in vitro ablate tumors vivo. ‐350R originates good activities vacancy‐rich nanoclusters.

Language: Английский

Citations

43
Tuning oxidant and antioxidant activities of ceria by anchoring copper single-site for antibacterial application DOI Creative Commons
Peng Jiang, Ludan Zhang, Xiaolong Liu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 3, 2024

Abstract The reaction system of hydrogen peroxide (H 2 O ) catalyzed by nanozyme has a broad prospect in antibacterial treatment. However, the complex catalytic activities nanozymes lead to multiple pathways reacting parallel, causing uncertain results. New approach effectively regulate is urgent need. Herein, Cu single site modified on nanoceria with various activities, such as peroxidase-like activity (POD) and hydroxyl radical antioxidant capacity (HORAC). Benefiting from interaction between coordinated CeO substrate, POD enhanced while HORAC inhibited, which further confirmed density functional theory (DFT) calculations. Cu-CeO + H shows good properties both vitro vivo. In this work, strategy based metal carrier provides general clue for optimizing nanozymes.

Language: Английский

Citations

42