Highly
sensitive
lateral
flow
immunoassays
(LFIAs)
are
essential
for
various
point-of-care
applications,
and
gold
nanoparticles
(Au
NPs)
by
far
the
most
commonly
used
labels.
However,
conventional
LFIAs
often
suffer
from
high
detection
limits
(LOD)
or
low
sensitivity.
In
this
study,
we
investigated
three
strategies
to
enhance
sensitivity
of
improving
peroxidase-mimicking
(POD)
activity
Au
NPs.
The
POD
unmodified
NPs
was
negligible
(<0.01
units/mg,
U/mg).
first
strategy
involved
coupling
with
horseradish
peroxidase
(HRP),
which
increased
65
U/mg.
second
approach
forming
a
thin
palladium
iridium
shell
on
NPs,
elevated
0.69-0.71
third
binding
mercury
ions
(Hg2+)
resulting
in
up
3
Finally,
developed
simple
quantitative
model
estimate
LOD
based
kinetic
parameters.
Using
Au-HRP
conjugates,
demonstrated
that
experimentally
measured
consistent
calculated
values.
provides
framework
evaluating
catalytic
signal
amplification
can
be
guide
development
highly
assays.
Advanced Materials,
Год журнала:
2023,
Номер
36(10)
Опубликована: Апрель 5, 2023
Metabolism
is
the
sum
of
enzyme-dependent
chemical
reactions,
which
produces
energy
in
catabolic
process
and
synthesizes
biomass
anabolic
process,
exhibiting
high
similarity
mammalian
cell,
microbial
plant
cell.
Consequently,
loss
or
gain
metabolic
enzyme
activity
greatly
affects
cellular
metabolism.
Nanozymes,
as
emerging
mimics
with
diverse
functions
adjustable
catalytic
activities,
have
shown
attractive
potential
for
regulation.
Although
basic
tasks
are
highly
similar
cells
from
different
species,
concrete
pathway
varies
intracellular
structure
species.
Here,
metabolism
living
organisms
described
similarities
differences
pathways
among
mammalian,
microbial,
regulation
mechanism
discussed.
The
recent
progress
on
mainly
including
nutrient
uptake
utilization,
production,
accompanied
redox
reactions
by
kinds
oxidoreductases
their
applications
field
disease
therapy,
antimicrobial
sustainable
agriculture
systematically
reviewed.
Furthermore,
prospects
challenges
nanozymes
regulating
cell
also
discussed,
broaden
application
scenarios.
Nano-Micro Letters,
Год журнала:
2024,
Номер
16(1)
Опубликована: Фев. 6, 2024
Inflammatory
skin
disorders
can
cause
chronic
scarring
and
functional
impairments,
posing
a
significant
burden
on
patients
the
healthcare
system.
Conventional
therapies,
such
as
corticosteroids
nonsteroidal
anti-inflammatory
drugs,
are
limited
in
efficacy
associated
with
adverse
effects.
Recently,
nanozyme
(NZ)-based
hydrogels
have
shown
great
promise
addressing
these
challenges.
NZ-based
possess
unique
therapeutic
abilities
by
combining
benefits
of
redox
nanomaterials
enzymatic
activity
water-retaining
capacity
hydrogels.
The
multifaceted
effects
include
scavenging
reactive
oxygen
species
other
inflammatory
mediators
modulating
immune
responses
toward
pro-regenerative
environment
enhancing
regenerative
potential
triggering
cell
migration
differentiation.
This
review
highlights
current
state
art
NZ-engineered
(NZ@hydrogels)
for
regeneration
applications.
It
also
discusses
underlying
chemo-mechano-biological
mechanisms
behind
their
effectiveness.
Additionally,
challenges
future
directions
this
ground,
particularly
clinical
translation,
addressed.
insights
provided
aid
design
engineering
novel
hydrogels,
offering
new
possibilities
targeted
personalized
skin-care
therapies.
CsPbX3
perovskite
nanocrystal
(NC)
is
considered
as
an
excellent
optical
material
and
widely
applied
in
optoelectronics.
However,
its
poor
water
stability
impedes
study
enzyme-like
activity,
further
inhibits
application
biomimetic
cascade
catalysis.
Herein,
for
the
first
time,
oxidase-like
ascorbate
activities
of
amphiphilic
polymer
capped
are
demonstrated,
catalytic
mechanism
explored.
Furthermore,
all-nanozyme
system
(multifunctional
CsPbBr3
@Zr-metal
organic
framework
(Zr-MOF)
Prussian
blue
peroxidase-like
nanozyme)
constructed
with
a
portable
paper-based
device
realizing
dual-mode
(ratiometric
fluorescence
colorimetric)
detection
ascorbic
acid
point-of-care
(POC)
fashion.
This
report
on
utilization
all-inorganic
NC
catalysis,
which
opens
new
avenue
POC
clinical
disease
diagnosis.
Abstract
Nanozymes
show
great
promise
in
enhancing
disease
biomarker
sensing
by
leveraging
their
physicochemical
properties
and
enzymatic
activities.
These
qualities
facilitate
signal
amplification
matrix
effects
reduction,
thus
boosting
performance.
In
this
review,
recent
studies
from
the
last
five
years,
concentrating
on
detection
improvement
through
nanozyme‐based
biosensing
are
examined.
This
enhancement
primarily
involves
modulations
of
size,
morphology,
doping,
modification,
electromagnetic
mechanisms,
electron
conduction
efficiency,
surface
plasmon
resonance
nanozymes
for
increased
sensitivity.
addition,
a
comprehensive
description
synthesis
tuning
strategies
employed
has
been
provided.
includes
detailed
elucidation
catalytic
mechanisms
alignment
with
fundamental
principles
enhanced
technology,
accompanied
presentation
quantitatively
analyzed
results.
Moreover,
diverse
applications
strip
sensing,
colorimetric
electrochemical
surface‐enhanced
Raman
scattering
have
outlined.
Additionally,
limitations,
challenges,
corresponding
recommendations
concerning
application
summarized.
Furthermore,
insights
offered
into
future
development
outlook
biosensing.
review
aims
to
serve
not
only
as
reference
sensitivity
biosensors
but
also
catalyst
exploring
nanozyme
broader
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(20), С. 15493 - 15493
Опубликована: Окт. 23, 2023
Bacterial
antibiotic
resistance,
especially
the
emergence
of
multidrug-resistant
(MDR)
strains,
urgently
requires
development
effective
treatment
strategies.
It
is
always
interest
to
delve
into
mechanisms
resistance
current
antibiotics
and
target
them
promote
efficacy
existing
antibiotics.
In
recent
years,
non-antibiotic
compounds
have
played
an
important
auxiliary
role
in
improving
promoting
drug-resistant
bacteria.
The
combination
with
considered
a
promising
strategy
against
MDR
this
review,
we
first
briefly
summarize
main
addition,
propose
several
strategies
enhance
action
based
on
mechanisms.
Then,
research
progress
that
can
antibiotic-resistant
bacteria
through
different
years
also
summarized.
Finally,
prospects
challenges
these
are
discussed.
Traditional
antibacterial
agents
are
often
observed
to
be
ineffective
because
bacteria
evolved
strains
with
greater
antibiotic
resistance.
Here,
vigorous
chitosan‐stabilized
PtAu
nanoparticles
(CSPA)
multienzyme‐like
activity
successfully
fabricated,
which
serve
an
effective
artificial
nanozyme
enhance
for
mixed
bacterial
infection
wound
treatment.
Ultrasmall
size
CSPA
exhibits
excellent
hydrophilicity
and
biocompatibility,
possesses
strong
oxidase‐
peroxidase‐like
generating
a
substantial
amount
of
ROS
(,
1
O
2
,
·OH)
cause
oxidative
damage
bacteria,
also
demonstrates
nicotinamide
adenine
dinucleotide
dehydrogenase‐like
disrupting
the
respiratory
chains,
subsequently
impedes
adenosine
triphosphate
production.
favorable
broad‐spectrum
at
very
low
concentrations,
prevents
resistance,
completely
inhibits
biofilm
formation.
Antibacterial
Mechanism
by
transcriptomics
is
further
revealed
that
can
induce
stress,
hinder
energy
metabolism,
disrupt
synthesis
function
cell
walls
membranes.
In
vivo,
population
site
promotes
healing
in
rats.
This
study
introduces
novel
approach,
providing
important
insight
into
mechanism
nanozymes
promoting
advancement
nanocatalytic
materials
biomedical
applications.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(20)
Опубликована: Март 26, 2024
The
emerging
cell
death
modality
of
ferroptosis
has
garnered
increasing
attention
for
antitumor
treatment
but
still
suffers
from
low
therapeutic
efficacy.
A
metal-organic
frameworks
(MOFs)-based
magnetic
nanozyme
(PZFH)
comprising
porphyrin-based
Zr-MOF
(PCN)
on
zinc
ferrite
(ZF)
nanoparticles
modified
with
hyaluronic
acid,
delivering
excellent
magnetophotonic
response
efficient
ferroptosis,
is
reported
here.
PZFH
shows
multienzyme-like
cascade
activity
encompassing
a
photon-triggered
oxidase-like
catalysis
to
generate
O
