Conventional Anthelmintic Concentration of Deltamethrin Immersion Disorder in the Gill Immune Responses of Crucian Carp DOI Creative Commons
Hao Wu,

Xiping Yuan,

Jinwei Gao

и другие.

Toxics, Год журнала: 2023, Номер 11(9), С. 743 - 743

Опубликована: Авг. 31, 2023

Current treatment strategies for parasitic infectious diseases in crucian carp primarily rely on chemotherapy. As a commonly used antiparasitic agent, deltamethrin (DEL) may have the potential adverse effects external mucosa of fish such as gills. In this study, 180 healthy juvenile (Carassius auratus) (average weight: 8.8 ± 1.0 g) were randomly divided into three groups 28 days, which immersed 0 μg/L, 0.3 and 0.6 μg/L DEL, respectively. The results histological analysis revealed that severe hyperplasia secondary lamellae gills was observed, number goblet (mucus-secreting) cells increased significantly after DEL immersion. TUNEL staining indicated apoptotic gill. At molecular level, mRNA expression significant upregulation apoptosis (caspase 3, caspase 8, bax), autophagy (atg5 beclin-1), immune response (lzm, muc5, il-6, il-8, il-10, tnfα, ifnγ, tgfβ, tlr4, myd88, nf-kb), whereas tight junction-related genes (occludin claudin12) downregulated immersion, suggesting immersion altered innate immunity responses promoted mucus secretion. Moreover, tandem mass tag (TMT)-based proteomics total 428 differentially expressed proteins (DEPs) contained 341 upregulated DEPs 87 with function annotation identified between control groups. Functional analyses enriched process, phagosome, lysosome pathways. Additionally, also drove gill microbiota to dysbiosis an increase potentially harmful bacteria Flavobacterium. Overall, study showed elicited shifts changes surface fish. These provide new perspectives conventional anthelmintic concentration disorder microenvironment theoretical support future optimization their practical application.

Язык: Английский

4-tert-butylphenol triggers common carp hepatocytes ferroptosis via oxidative stress, iron overload, SLC7A11/GSH/GPX4 axis, and ATF4/HSPA5/GPX4 axis DOI Creative Commons
Jiawen Cui, Qin Zhou,

Meijin Yu

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2022, Номер 242, С. 113944 - 113944

Опубликована: Авг. 1, 2022

4-tert-butylphenol (4-tBP) is a toxic environmental pollutant with moderate bioaccumulation, persistence, and long-term toxicity. Its toxicity to aquatic organisms has become an issue of concern. However, the molecular mechanism 4-tBP remained unclear. Liver target organ for pollutants. Here, we established 4-tBP-exposed model in vivo primary hepatocyte vitro common carp (Cyprinus carpio L.). We found increased hepatic-somatic index (HSI) abnormal serum biochemical indexes (ALT, AST, LDH) after exposure, indicating liver damage. further revealed that damaged structural integrity livers typical features ferroptosis. Based on toxicogenomics analysis, ferroptosis likely be involved 4-tBP-induced Moreover, our experiment provided evidences 4-tBP-exposure led excess oxidative stress, iron overload, decreased MMP, expression ferroptosis-related factors. Interestingly, ferrostatin-1 (Fer-1, inhibitor) pretreatment alleviated above changes. In summary, demonstrated triggered hepatocytes via SLC7A11/GSH/GPX4 axis, ATF4/HSPA5/GPX4 axis. For first time, discovered Fer-1 can ameliorate 4-tBP, which needs more investigations. Our results scientific basis fish poisoning.

Язык: Английский

Процитировано

75
Acute exposure to polystyrene nanoplastics induced oxidative stress in Sepia esculenta Larvae DOI Creative Commons
Xiumei Liu, Zan Li, Qian Li

и другие.

Aquaculture Reports, Год журнала: 2024, Номер 35, С. 102004 - 102004

Опубликована: Фев. 28, 2024

Nanoplastics (NPs, < 1 μm) have attracted widespread attention due to their potential threats organisms, ecosystem, and public health. However, there is limited research on the effects of NPs cephalopods. Sepia esculenta (Mollusks, Cephalopoda, Sepioidea), an economically major species Sepiidea family in world, well received by consumers for its delicious quality rich nutrition. In present study, S. larvae were treated with polystyrene nanoplastics (PS-NPs) at 50 μg/mL 4 h, then physiological biomarkers (SOD, GSTs MDA) RNA-Seq whole body performed. Research results suggested that PS-NPs exposure had induced oxidative stress larvae. addition, several key genes signaling pathways related obtained based DEGs, GO KEGG enrichment analyses, as PPI construction analysis transcriptome data. This study enriches different organisms provides insights references revealing mechanisms caused molecular level.

Язык: Английский

Процитировано

22
Toll-like receptor signaling in teleosts DOI
Jianguo Su

Science China Life Sciences, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Язык: Английский

Процитировано

2
Nano-selenium protects grass carp hepatocytes against 4-tert-butylphenol-induced mitochondrial apoptosis and necroptosis via suppressing ROS-PARP1 axis DOI
Jiawen Cui,

Minna Qiu,

Yuhang Liu

и другие.

Fish & Shellfish Immunology, Год журнала: 2023, Номер 135, С. 108682 - 108682

Опубликована: Март 14, 2023

Язык: Английский

Процитировано

41
EGCG alleviated Mn exposure-caused carp kidney damage via trpm2-NLRP3-TNF-α-JNK pathway: Oxidative stress, inflammation, and tight junction dysfunction DOI
Qin Zhou, Jiawen Cui, Yuhang Liu

и другие.

Fish & Shellfish Immunology, Год журнала: 2023, Номер 134, С. 108582 - 108582

Опубликована: Фев. 7, 2023

Язык: Английский

Процитировано

38
The effect of acute toxicity from tributyltin on Liza haematocheila liver: Energy metabolic disturbance, oxidative stress, and apoptosis DOI

Changsheng Zhao,

Xiaohua Teng,

Weizhong Yue

и другие.

Aquatic Toxicology, Год журнала: 2023, Номер 258, С. 106506 - 106506

Опубликована: Март 21, 2023

Язык: Английский

Процитировано

38
Effect of arsenic stress on the intestinal structural integrity and intestinal flora abundance of Cyprinus carpio DOI Creative Commons

Xiaodan Shi,

Wei Xu,

Xinghua Che

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Апрель 24, 2023

Aquatic organisms such as fish can accumulate high concentrations of arsenic (As), which has toxic effects on fish. However, whether the intestinal flora are involved in As damage to tissues and underlying process unclear. Common carp (Cyprinus carpio) were exposed (2.83 mg/L) water for 30 days, blood, muscle, intestine, intestine samples collected. Intestinal pathological sections observed, lipopolysaccharide (LPS) levels serum accumulation tight junction-related factors measured. The gut microbiota was analysed by 16S rRNA sequencing. results showed that treatment decreased abundance microbiota, increased number harmful bacteria, beneficial bacteria intestine. In our experiment, top with highest relative identified. Among resulted a significant (P < 0.05) increase (such Fusobacteriota, Bacteroidota (LPS-producing bacteria), Verrucomicrobiota, Bacteroides, Aeromonas, Stenotrophomonas) decrease Actinobacteriota, Planctomycetota, Firmicutes, Reyranella, Akkermansia, Pseudorhodobacter), further demonstrated affects flora. addition, exposure LPS level bacteria) exhibits six at phylum level, indicates produced serum. Additionally, protein gene junction markers ZO-1 occludin reduced treatment, indicated impaired structural integrity conclusion, obtained study indicate flora, LPS, junctions participate impairment common resulting from exposure.

Язык: Английский

Процитировано

37
The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens DOI Creative Commons
Bing Yang, Xiaofeng Li, Noura M. Mesalam

и другие.

Poultry Science, Год журнала: 2024, Номер 103(6), С. 103675 - 103675

Опубликована: Март 22, 2024

Polygonatum sibiricum polysaccharide (PSP) has demonstrated diverse medicinal properties, extensively researched for human applications. Nonetheless, there is a lack of studies investigating the potential advantages PSP in poultry farming. The present study investigated impact incorporating into broiler diets on their growth performance, meat quality, blood metabolites, antioxidative status, and ileal histomorphology. Two hundred forty-one-day-old male Ross-308 chicks (44.98 ± 0.79 g) were randomly assigned to 3 experimental groups, with 8 replicates 10 birds each. fed supplemented at 0, 400, 800 mg/kg (control, PSP400, PSP800, respectively). results revealed linear (P > 0.05) improvement body weight gain, European production efficiency index, feed conversion ratio during grower (22-35 d) overall periods (1-35 d). pH levels ingluvies, ileum, cecum exhibited reduction PSP800 group d 21 35, respectively. Villus height crypt depth increased PSP400 groups compared control group. decreased hydrogen peroxide (H

Язык: Английский

Процитировано

11
The toxicity of 4-tert-butylphenol in early development of zebrafish: morphological abnormality, cardiotoxicity, and hypopigmentation DOI
Mingxing Wang, Tian Qin, Guoliang Chen

и другие.

Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(16), С. 45781 - 45795

Опубликована: Янв. 28, 2023

Язык: Английский

Процитировано

14
The effects of ACE inhibitor Enalapril on Mytilus galloprovincialis: Insights into morphological and functional responses DOI Creative Commons
Mariacristina Filice,

Alessia Caferro,

Daniela Amelio

и другие.

Aquatic Toxicology, Год журнала: 2024, Номер 273, С. 107014 - 107014

Опубликована: Июнь 27, 2024

In the last decades, pharmaceuticals have emerged as a new class of environmental contaminants. Antihypertensives, including angiotensin-converting enzyme (ACE) inhibitors, are special concern due to their increased consumption over past years. However, available data on putative effects health aquatic animals, well possible interaction with biological systems still poorly understood. This study analysed whether and which extent exposure Enalapril, an ACE inhibitor commonly used for treating hypertension heart failure, may induce morpho-functional alterations in mussel Mytilus galloprovincialis, sentinel organism water pollution. By mainly focusing digestive gland (DG), target tissue analysing xenobiotics mussels, 10-days 0.6 ng/L (E1) 600 (E2) Enalapril were investigated terms cell viability volume regulation, morphology, oxidative stress, stress protein expression localization. Results indicated that compromised capacity DG cells from E2 group regulate by limiting ability return original after hypoosmotic stress. occurred without significant viability. unaffected also haemocytes viability, although infiltration was histologically observed both groups, suggestive immune response. No changes two experimental groups localization heat shock proteins 70 (HSPs70) HSP90, levels biomarkers. Our results showed that, M. galloprovincialis did not influence status, stress-related proteins, while it activated response face osmotic changes, potential consequences animal performance.

Язык: Английский

Процитировано

5