SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Biosensors and Bioelectronics, Год журнала: 2022, Номер 207, С. 114167 - 114167

Опубликована: Март 17, 2022

Язык: Английский

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Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example

Biosensors and Bioelectronics, Год журнала: 2022, Номер 206, С. 114109 - 114109

Опубликована: Фев. 26, 2022

Язык: Английский

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Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Biosensors and Bioelectronics, Год журнала: 2022, Номер 215, С. 114559 - 114559

Опубликована: Июль 13, 2022

Язык: Английский

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A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics

Biosensors and Bioelectronics, Год журнала: 2021, Номер 195, С. 113646 - 113646

Опубликована: Сен. 23, 2021

Язык: Английский

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CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2022, Номер 21(4), С. 3770 - 3798

Опубликована: Июль 1, 2022

Abstract Food safety is one of the biggest public issues occurring around world. Microbiological, chemical, and physical hazards can lead to food issues, which may occur at all stages supply chain. In order tackle safeguard consumer health, rapid, accurate, specific, field‐deployable detection methods meeting diverse requirements are imperative measures for assurance. CRISPR‐Cas system, a newly emerging technology, has been successfully repurposed in biosensing demonstrated huge potential establish conceptually novel with high sensitivity specificity. This review focuses on CRISPR‐Cas–based its current status specifically inspection. We firstly illustrate pending problems summarize popular methods. then describe applications Finally, challenges futuristic opportunities proposed discussed. Generally speaking, still unsatisfactory some ways such as being time‐consuming, displaying unmet specificity standards, there comparative paucity multiplexed testing POCT. Recent studies have shown that an innovative fast‐expanding could make up shortcomings existing or even replace them. To sum up, implementation integration other techniques promising desirable, expected provide “customized” “smart” inspection coming future.

Язык: Английский

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Integrating CRISPR/Cas within isothermal amplification for point-of-Care Assay of nucleic acid

Talanta, Год журнала: 2022, Номер 243, С. 123388 - 123388

Опубликована: Март 12, 2022

Язык: Английский

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A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel

Journal of Hazardous Materials, Год журнала: 2023, Номер 452, С. 131195 - 131195

Опубликована: Март 11, 2023

Язык: Английский

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Advancements in CRISPR-Based Biosensing for Next-Gen Point of Care Diagnostic Application

Biosensors, Год журнала: 2023, Номер 13(2), С. 202 - 202

Опубликована: Янв. 29, 2023

With the move of molecular tests from diagnostic labs to on-site testing becoming more common, there is a sudden rise in demand for nucleic acid-based tools that are selective, sensitive, flexible terrain changes, and cost-effective assist point-of-care systems large-scale screening be used remote locations cases outbreaks pandemics. CRISPR-based biosensors comprise promising new approach acid detection, which uses Cas effector proteins (Cas9, Cas12, Cas13) as extremely specialized identification components may conjunction with variety readout approaches (such fluorescence, colorimetry, potentiometry, lateral flow assay, etc.) onsite analysis. In this review, we cover some technical aspects integrating CRISPR system traditional biosensing methods amplification technologies such polymerase chain reaction (PCR), loop-mediated isothermal (LAMP), recombinase (RPA) continue elaborate on prospects developed biosensor detection major viral bacterial diseases. Within scope article, also discuss recent COVID pandemic numerous have undergone development since its advent. Finally, challenges future testing.

Язык: Английский

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Sensitive Small Molecule Aptasensing based on Hybridization Chain Reaction and CRISPR/Cas12a Using a Portable 3D-Printed Visualizer

ACS Sensors, Год журнала: 2023, Номер 8(3), С. 1076 - 1084

Опубликована: Янв. 18, 2023

Next-generation biosensing tools based on CRISPR/Cas have revolutionized the molecular detection. A number of CRISPR/Cas-based biosensors been reported for detection nucleic acid targets. The establishment efficient methods non-nucleic target would further broaden scope this technique, but up to now, concerning research is limited. In current study, we a versatile platform small-molecule called SMART-Cas12a (small-molecule aptamer regulated test using CRISPR/Cas12a). Simply, hybridization chain reaction cascade signal amplification was first trigged by functional (aptamer) through binding. Then, system integrated recognize amplified products followed activation trans-cleavage. As such, can be ingeniously converted signals and then fluorescent that readily visualized analyzed customized 3D-printed visualizer with help home-made App-enabled smartphone. Adenosine triphosphate selected as model target, under optimized conditions, achieved fine analytical performance linear range from 0.1 750 μM limit 1.0 nM. satisfactory selectivity recoveries obtained demonstrated method suitable complex sample environment. sample-to-answer time less than 100 min. Our work not only expanded reach CRISPR-Cas in also provided prototype generalized detecting wider analytes desirable adaptability, sensitivity, specificity, on-site capability.

Язык: Английский

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CRISPR-Microfluidics Nexus: Advancing Biomedical Applications for Understanding and Detection

Sensors and Actuators A Physical, Год журнала: 2024, Номер 376, С. 115625 - 115625

Опубликована: Июнь 24, 2024

The integration of CRISPR technology with microfluidic-based biosensors has greatly expanded its applications in medicine and molecular biology. This combination offers enhanced sensitivity selectivity medical diagnostics. CRISPR-powered microfluidics enables the accurate quantification DNA RNA associated diseases such as cancer, viral infections, bacterial diseases. precise targeting capabilities allow for detection specific genetic sequences, aiding early diagnosis, disease monitoring, treatment assessment. improves specificity by leveraging programmable nature coupling it signal readouts. However, challenges assay optimization, standardization, device fabrication need to be addressed. Additionally, complexity presents potential limitations terms off-target effects unintended modifications. Overall, holds tremendous diagnostics, but further research development are required fully exploit benefits clinical settings.

Язык: Английский

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