ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(12), P. 4420 - 4441
Published: Nov. 18, 2023
CRISPR/Cas
system
is
becoming
an
increasingly
influential
technology
that
has
been
repositioned
in
nucleic
acid
detection.
A
preamplification
step
usually
required
to
improve
the
sensitivity
of
CRISPR/Cas-based
The
striking
biological
features
CRISPR/Cas,
including
programmability,
high
and
sequence
specificity,
single-base
resolution.
More
strikingly,
target-activated
trans-cleavage
could
act
as
a
biocatalytic
signal
transductor
amplifier,
thereby
empowering
it
potentially
perform
detection
without
step.
reports
such
work
are
on
rise,
which
not
only
scientifically
significant
but
also
promising
for
futuristic
end-user
applications.
This
review
started
with
introduction
methods
acids
diagnostics
(CRISPR-Dx).
Next,
we
objectively
discussed
pros
cons
steps
CRISPR-Dx.
We
then
illustrated
highlighted
recently
developed
strategies
CRISPR/Cas-powered
amplification-free
can
be
realized
through
uses
ultralocalized
reactors,
cascade
reactions,
ultrasensitive
systems,
or
others.
Lastly,
challenges
perspectives
were
proposed.
It
expected
this
makes
researchers
better
understand
current
emerging
field,
provides
insight
designing
novel
CRISPR-Dx
win
practicable
use
near
future.
Comprehensive Reviews in Food Science and Food Safety,
Journal Year:
2022,
Volume and Issue:
21(4), P. 3770 - 3798
Published: July 1, 2022
Abstract
Food
safety
is
one
of
the
biggest
public
issues
occurring
around
world.
Microbiological,
chemical,
and
physical
hazards
can
lead
to
food
issues,
which
may
occur
at
all
stages
supply
chain.
In
order
tackle
safeguard
consumer
health,
rapid,
accurate,
specific,
field‐deployable
detection
methods
meeting
diverse
requirements
are
imperative
measures
for
assurance.
CRISPR‐Cas
system,
a
newly
emerging
technology,
has
been
successfully
repurposed
in
biosensing
demonstrated
huge
potential
establish
conceptually
novel
with
high
sensitivity
specificity.
This
review
focuses
on
CRISPR‐Cas–based
its
current
status
specifically
inspection.
We
firstly
illustrate
pending
problems
summarize
popular
methods.
then
describe
applications
Finally,
challenges
futuristic
opportunities
proposed
discussed.
Generally
speaking,
still
unsatisfactory
some
ways
such
as
being
time‐consuming,
displaying
unmet
specificity
standards,
there
comparative
paucity
multiplexed
testing
POCT.
Recent
studies
have
shown
that
an
innovative
fast‐expanding
could
make
up
shortcomings
existing
or
even
replace
them.
To
sum
up,
implementation
integration
other
techniques
promising
desirable,
expected
provide
“customized”
“smart”
inspection
coming
future.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(2), P. 202 - 202
Published: Jan. 29, 2023
With
the
move
of
molecular
tests
from
diagnostic
labs
to
on-site
testing
becoming
more
common,
there
is
a
sudden
rise
in
demand
for
nucleic
acid-based
tools
that
are
selective,
sensitive,
flexible
terrain
changes,
and
cost-effective
assist
point-of-care
systems
large-scale
screening
be
used
remote
locations
cases
outbreaks
pandemics.
CRISPR-based
biosensors
comprise
promising
new
approach
acid
detection,
which
uses
Cas
effector
proteins
(Cas9,
Cas12,
Cas13)
as
extremely
specialized
identification
components
may
conjunction
with
variety
readout
approaches
(such
fluorescence,
colorimetry,
potentiometry,
lateral
flow
assay,
etc.)
onsite
analysis.
In
this
review,
we
cover
some
technical
aspects
integrating
CRISPR
system
traditional
biosensing
methods
amplification
technologies
such
polymerase
chain
reaction
(PCR),
loop-mediated
isothermal
(LAMP),
recombinase
(RPA)
continue
elaborate
on
prospects
developed
biosensor
detection
major
viral
bacterial
diseases.
Within
scope
article,
also
discuss
recent
COVID
pandemic
numerous
have
undergone
development
since
its
advent.
Finally,
challenges
future
testing.
Sensors and Actuators A Physical,
Journal Year:
2024,
Volume and Issue:
376, P. 115625 - 115625
Published: June 24, 2024
The
integration
of
CRISPR
technology
with
microfluidic-based
biosensors
has
greatly
expanded
its
applications
in
medicine
and
molecular
biology.
This
combination
offers
enhanced
sensitivity
selectivity
medical
diagnostics.
CRISPR-powered
microfluidics
enables
the
accurate
quantification
DNA
RNA
associated
diseases
such
as
cancer,
viral
infections,
bacterial
diseases.
precise
targeting
capabilities
allow
for
detection
specific
genetic
sequences,
aiding
early
diagnosis,
disease
monitoring,
treatment
assessment.
improves
specificity
by
leveraging
programmable
nature
coupling
it
signal
readouts.
However,
challenges
assay
optimization,
standardization,
device
fabrication
need
to
be
addressed.
Additionally,
complexity
presents
potential
limitations
terms
off-target
effects
unintended
modifications.
Overall,
holds
tremendous
diagnostics,
but
further
research
development
are
required
fully
exploit
benefits
clinical
settings.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(6), P. 2676 - 2683
Published: Jan. 30, 2024
Sepsis
is
an
extremely
dangerous
medical
condition
that
emanates
from
the
body's
response
to
a
pre-existing
infection.
Early
detection
of
sepsis-inducing
bacterial
infections
can
greatly
enhance
treatment
process
and
potentially
prevent
onset
sepsis.
However,
current
point-of-care
(POC)
sensors
are
often
complex
costly
or
lack
ideal
sensitivity
for
effective
detection.
Therefore,
it
crucial
develop
rapid
sensitive
biosensors
on-site
bacteria.
Herein,
we
developed
graphene
oxide
CRISPR-Cas12a
(GO-CRISPR)
biosensor
bacteria
in
human
serum.
In
this
strategy,
single-stranded
(ssDNA)
FAM
probes
were
quenched
with
single-layer
(GO).
Target-activated
Cas12a
trans-cleavage
was
utilized
degradation
ssDNA
probes,
detaching
short
GO
recovering
fluorescent
signals.
Under
optimal
conditions,
employed
our
GO-CRISPR
system
Salmonella
Typhimurium
(S.
Typhimurium)
as
low
3
×
103
CFU/mL
serum,
well
good
specificity
toward
other
competing
addition,
exhibited
excellent
S.
spiked
The
offers
superior
rapidity
has
potential
early
resource-limited
settings,
expediting
patients
at
risk
