Experience-dependent reorganization of inhibitory neuron synaptic connectivity DOI Creative Commons
Andrew J. P. Fink, Samuel P. Muscinelli, Shuqi Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Organisms continually tune their perceptual systems to the features they encounter in environment 1-3 . We have studied how ongoing experience reorganizes synaptic connectivity of neurons olfactory (piriform) cortex mouse. developed an approach measure vivo , training a deep convolutional network reliably identify monosynaptic connections from spike-time cross-correlograms 4.4 million single-unit pairs. This revealed that excitatory piriform with similar odor tuning are more likely be connected. asked whether enhances this like-to-like but found it was unaffected by exposure. Experience did, however, alter logic interneuron connectivity. Following repeated encounters set odorants, inhibitory responded differentially these stimuli exhibited high degree both incoming and outgoing within cortical network. reorganization depended only on not its pre- or postsynaptic partners. A computational model reorganized predicts increases dimensionality entire network's responses familiar stimuli, thereby enhancing discriminability. confirmed network-level property is present physiological measurements, which showed increased separability evoked versus novel odorants. Thus, simple, non-Hebbian may selectively enhance organism's discrimination environment.

Язык: Английский

Circuit formation and sensory perception in the mouse olfactory system DOI Creative Commons
Kensaku Mori, Hitoshi Sakano

Frontiers in Neural Circuits, Год журнала: 2024, Номер 18

Опубликована: Фев. 16, 2024

In the mouse olfactory system, odor information is converted to a topographic map of activated glomeruli in bulb (OB). Although arrangement genetically determined, glomerular structure plastic and can be modified by environmental stimuli. If pups are exposed particular odorant, responding become larger recruiting dendrites connecting projection neurons interneurons. This imprinting not only increases sensitivity odor, but also imposes positive quality on imprinted memory. External represented as an OB transmitted cortex (OC) amygdala for decision making elicit emotional behavioral outputs using two distinct neural pathways, innate learned. Innate circuits start work right after birth, whereas learned functional later on. this paper, recent progress will summarized study circuit formation perception mice. We propose new hypotheses timing gating activity relation respiration cycle.

Язык: Английский

Процитировано

6
Experience-dependent evolution of odor mixture representations in piriform cortex DOI Creative Commons
Alice Berners-Lee, Elizabeth Shtrahman, Julien Grimaud

и другие.

PLoS Biology, Год журнала: 2023, Номер 21(4), С. e3002086 - e3002086

Опубликована: Апрель 25, 2023

Rodents can learn from exposure to rewarding odors make better and quicker decisions. The piriform cortex is thought be important for learning complex odor associations; however, it not understood exactly how learns remember discriminations between many, sometimes overlapping, mixtures. We investigated mixtures are represented in the posterior (pPC) of mice while they discriminate a unique target mixture against hundreds nontarget find that significant proportion pPC neurons all other Neurons prefer tend respond with brief increases firing rate at onset compared neurons, which exhibit sustained and/or decreased firing. allowed continue training after had reached high levels performance become more selective as well randomly chosen repeated did have nontargets. These single unit changes during overtraining accompanied by categorization decoding population level, even though behavioral metrics such reward latency do change. However, when difficult ambiguous trial types introduced, robustness selectivity correlated on trials. Taken together, these data reveal dynamic robust system optimize both current possible future task demands once.

Язык: Английский

Процитировано

13
Opposing, spatially-determined epigenetic forces impose restrictions on stochastic olfactory receptor choice DOI Creative Commons

Elizaveta Bashkirova,

Nell Klimpert, Kevin D. Monahan

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Май 12, 2023

Olfactory receptor (OR) choice represents an example of genetically hardwired stochasticity, where every olfactory neuron expresses one out ~2000 OR alleles in the mouse genome a probabilistic, yet stereotypic fashion. Here, we propose that topographic restrictions expression are established neuronal progenitors by two opposing forces: polygenic transcription and genomic silencing, both which influenced dorsoventral gradients factors NFIA, B, X. Polygenic genes may define spatially constrained repertoires, among allele is selected for singular later development. Heterochromatin assembly compartmentalization also vary across axes epithelium preferentially eliminate ectopically expressed ORs with more dorsal destinations from this ‘privileged’ repertoire. Our experiments identify early as potential ‘epigenetic’ contributor to future developmental patterning reveal how responsive probabilistic processes act concert establish deterministic, precise, reproducible territories stochastic gene expression.

Язык: Английский

Процитировано

13
Global Sugar Reduction Trends and Challenges: Exploring Aroma Sweetening as an Alternative to Sugar Reduction DOI

Di Zhao,

Yue Chen,

Jiayi Xia

и другие.

Trends in Food Science & Technology, Год журнала: 2024, Номер 150, С. 104602 - 104602

Опубликована: Авг. 1, 2024

Язык: Английский

Процитировано

5
Integrative Transcriptomic Analyses of Hippocampal–Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease DOI Creative Commons

Dan Luo,

Jingying Li,

Hanyou Liu

и другие.

Advanced Science, Год журнала: 2023, Номер 10(22)

Опубликована: Май 26, 2023

Abstract The hippocampal–entorhinal system supports cognitive function and is selectively vulnerable to Alzheimer's disease (AD). Little known about global transcriptomic changes in the subfields during AD. Herein, large‐scale analysis performed five of postmortem brain tissues (262 unique samples). Differentially expressed genes are assessed across states, integrated genotype data from an AD genome‐wide association study. An integrative gene network bulk single‐nucleus RNA sequencing (snRNA‐Seq) identifies with causative roles progression. Using a system‐biology approach, pathology‐specific expression patterns for cell types demonstrated, notably upregulation A1‐reactive astrocyte signature entorhinal cortex (EC) SnRNA‐Seq show that PSAP signaling involved alterations cell– communications EC Further experiments validate key role inducing astrogliosis A1‐like reactive phenotype. In summary, this study reveals subfield‐, type‐, demonstrates as potential therapeutic target

Язык: Английский

Процитировано

11
Opposing, spatially-determined epigenetic forces impose restrictions on stochastic olfactory receptor choice DOI Creative Commons

Elizaveta Bashkirova,

Nell Klimpert, Kevin D. Monahan

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 18, 2023

Olfactory receptor (OR) choice represents an example of genetically hardwired stochasticity, where every olfactory neuron expresses one out ~2000 OR alleles in the mouse genome a probabilistic, yet stereotypic fashion. Here, we propose that topographic restrictions expression are established neuronal progenitors by two opposing forces: polygenic transcription and genomic silencing, both which influenced dorsoventral gradients factors NFIA, B, X. Polygenic genes may define spatially constrained repertoires, among allele is selected for singular later development. Heterochromatin assembly compartmentalization also vary across axes epithelium preferentially eliminate ectopically expressed ORs with more dorsal destinations from this ‘privileged’ repertoire. Our experiments identify early as potential ‘epigenetic’ contributor to future developmental patterning reveal how responsive probabilistic processes act concert establish deterministic, precise, reproducible territories stochastic gene expression.

Язык: Английский

Процитировано

11
Neurogenesis dynamics in the olfactory bulb: deciphering circuitry organization, function, and adaptive plasticity DOI Creative Commons
Moawiah M. Naffaa

Neural Regeneration Research, Год журнала: 2024, Номер 20(6), С. 1565 - 1581

Опубликована: Июнь 26, 2024

Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to granule cell layer and glomerular layers of olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation these bulb supports both structural functional plasticity, aiding circuit remodeling triggered by memory learning processes. However, presence neurons, coupled cellular diversity within presents an ongoing challenge understanding its network organization function. Moreover, continuous integration plays a pivotal role regulating information processing. This adaptive process responds changes epithelial composition contributes formation memories modulating connectivity interacting intricately higher-order brain regions. adult functions remains topic debate. Nevertheless, functionality is linked cells around mitral tufted cells. organizational pattern significantly impacts output, behavior, synaptic which are crucial for perception memory. Additionally, this further shaped axon terminals originating from cortical subcortical Despite behaviors related olfaction, complex highly interconnected processes have not been comprehensively studied whole. Therefore, manuscript aims discuss our current explore how neural plasticity contribute enhancing adaptability system. These mechanisms thought support memory, potentially through increased complexity restructuring structures, well addition that aid adaptation. underscores importance employing precise methodologies elucidate specific roles amidst conflicting data varying experimental paradigms. Understanding essential gaining insights complexities function behavior.

Язык: Английский

Процитировано

4
Recruitment of grid-like responses in human entorhinal and piriform cortices by odor landmark-based navigation DOI Creative Commons
Clara U. Raithel, Alexander J. Miller, Russell A. Epstein

и другие.

Current Biology, Год журнала: 2023, Номер 33(17), С. 3561 - 3570.e4

Опубликована: Июль 27, 2023

Olfactory navigation is universal across the animal kingdom. Humans, however, have rarely been considered in this context. Here, we combined olfactometry techniques, virtual reality (VR) software, and neuroimaging methods to investigate whether humans can navigate an olfactory landscape by learning spatial relationships among discrete odor cues integrating knowledge into a map. Our data show that over time, participants improved their performance on task taking more direct paths toward targets completing trials within given time period. This suggests successfully complex odorous environment, reinforcing notion of human navigation. fMRI collected during revealed emergence grid-like responses entorhinal piriform cortices were attuned same grid orientation. result implies existence specialized network tasked with guiding based landmarks.

Язык: Английский

Процитировано

9
Shank3 Deficiency Results in a Reduction in GABAergic Postsynaptic Puncta in the Olfactory Brain Areas DOI Creative Commons
Denisa Mihalj, Veronika Borbélyová, Zdeno Pirník

и другие.

Neurochemical Research, Год журнала: 2024, Номер 49(4), С. 1008 - 1016

Опубликована: Янв. 6, 2024

Abstract Dysfunctional sensory systems, including altered olfactory function, have recently been reported in patients with autism spectrum disorder (ASD). Disturbances processing can potentially result from gamma-aminobutyric acid (GABA)ergic synaptic abnormalities. The specific molecular mechanism by which GABAergic transmission affects the system ASD remains unclear. Therefore, present study aimed to evaluate selected components of brain regions and primary neurons isolated Shank3 -deficient ( −/− ) mice, are known for their autism-like behavioral phenotype. deficiency led a significant reduction GEPHYRIN/GABA A R colocalization piriform cortex bulb, while no change cell morphology was observed. Gene expression analysis revealed mRNA levels GABA transporter 1 bulb Collybistin frontal mice compared WT mice. similar trend observed Somatostatin other neurotransmission markers did not yield statistically results. Overall, it appears that leads changes synapses important information processing, may represent basis understanding functional impairments autism.

Язык: Английский

Процитировано

3
Graded FGF activity patterns distinct cell types within the apical sensory organ of the sea anemone Nematostella vectensis DOI Creative Commons
Keith Z. Sabin,

Shiyuan Chen,

Eric M. Hill

и другие.

Developmental Biology, Год журнала: 2024, Номер 510, С. 50 - 65

Опубликована: Март 22, 2024

Bilaterian animals have evolved complex sensory organs comprised of distinct cell types that function coordinately to sense the environment. Each unit has a defined architecture built from component types, including cells, non-sensory support and dedicated neurons. Whether this characteristic cellular composition is present in non-bilaterian unknown. Here, we interrogate type gene regulatory networks controlling development larval apical organ sea anemone Nematostella vectensis. Using single RNA sequencing imaging approaches, reveal two unique organ, GABAergic cells putative population. Further, identify paired-like (PRD) homeodomain prd146 as specific marker show Prd146

Язык: Английский

Процитировано

3