Analytical Chemistry,
Год журнала:
2022,
Номер
94(11), С. 4787 - 4793
Опубликована: Март 11, 2022
Bladder
cancer
(BC)
is
one
of
the
most
common
cancers
in
world,
with
high
morbidity
and
mortality.
It
essential
to
develop
a
non-invasive,
highly
accurate,
simple
method
for
BC
diagnosis.
This
work
proposed
fluorescent
biosensor
based
on
inorganic
nanoflares
combined
DNAzyme
walker
simultaneous
detection
exosomal
microRNAs
(miRNAs).
was
constructed
Au
nanoparticle
(AuNP)
modified
carbon
dot
(CD)-labeled
substrates
strands
(AuNP@CDs
nanoflares-DNAzyme,
APCD).
In
presence
target
miRNAs,
activated
then
cleaved
CD-labeled
automatically
walked
along
AuNP,
allowing
fluorescence
recovery.
Due
structure
functional
composition,
APCD
biosensors
demonstrated
sensitivity
specificity,
reached
limit
single
miRNA
at
femtomolar
level
wide
linear
range
from
50
fM
10
nM.
Furthermore,
analysis
BC-related
miR-133b
miR-135b
clinical
serum
specimens
achieved
consistent
qRT-PCR,
suggesting
it
potential
diagnosis
other
cancers.
Cells,
Год журнала:
2023,
Номер
12(10), С. 1416 - 1416
Опубликована: Май 17, 2023
Extracellular
vesicles
(EVs)
such
as
ectosomes
and
exosomes
have
gained
attention
promising
natural
carriers
for
drug
delivery.
Exosomes,
which
range
from
30
to
100
nm
in
diameter,
possess
a
lipid
bilayer
are
secreted
by
various
cells.
Due
their
high
biocompatibility,
stability,
low
immunogenicity,
favored
cargo
carriers.
The
membrane
of
also
offers
protection
against
degradation,
making
them
desirable
candidate
However,
loading
into
remains
be
challenge.
Despite
strategies
incubation,
electroporation,
sonication,
extrusion,
freeze–thaw
cycling,
transfection
that
been
developed
facilitate
loading,
inadequate
efficiency
still
persists.
This
review
an
overview
current
delivery
using
summarizes
recent
approaches
small-molecule,
nucleic
acid,
protein
drugs
exosomes.
With
insights
these
studies,
we
provide
ideas
more
efficient
effective
molecules
Journal of Translational Medicine,
Год журнала:
2022,
Номер
20(1)
Опубликована: Июнь 27, 2022
Central
nervous
system
(CNS)
diseases,
such
as
multiple
sclerosis,
Alzheimer's
disease
(AD),
and
Parkinson's
(PD),
affect
millions
of
people
around
the
world.
Great
efforts
were
put
in
related
research,
but
few
breakthroughs
have
been
made
diagnostic
therapeutic
approaches.
Exosomes
are
cell-derived
extracellular
vesicles
containing
diverse
biologically
active
molecules
secreted
by
their
cell
origin.
These
contents,
including
nucleic
acids,
proteins,
lipids,
amino
metabolites,
can
be
transferred
between
different
cells,
tissues,
or
organs,
regulating
various
intercellular
cross-organ
communications
normal
pathogenic
processes.
Considering
that
cellular
environment
state
strongly
impact
content
uptake
efficiency
exosomes,
detection
biological
fluids
composition
analysis
potentially
offer
a
multicomponent
readout
several
human
diseases.
Recently,
studies
found
aberrant
secretion
exosomes
closely
to
pathogenesis
CNS
Besides,
loading
natural
cargoes,
deliver
drugs
cross
blood
brain
barrier,
making
them
emerging
candidates
biomarkers
therapeutics
for
In
this
review,
we
summarize
discuss
advanced
research
progress
pathological
processes
diseases
regarding
with
neuroinflammation,
repair,
protein
aggregation.
Moreover,
propose
strategies
applying
diagnosis,
early
detection,
treatment
Stem Cell Research & Therapy,
Год журнала:
2022,
Номер
13(1)
Опубликована: Июль 15, 2022
Human
urine-derived
stem
cells
(USCs)-derived
exosomes
(USC-Exo)
could
improve
kidney
ischemia/reperfusion
injury
(IRI),
while
the
underlying
mechanisms
of
this
protective
effect
remain
unclear.Human
USCs
and
USC-Exo
were
isolated
verified
by
morphology
specific
biomarkers.
The
effects
on
ferroptosis
detected
in
IRI-induced
acute
(AKI)
model
C57BL/6
mice.
lncRNA
taurine-upregulated
gene
1
(TUG1)
hypoxia/reoxygenation
(H/R)-treated
human
proximal
tubular
epithelial
(HK-2).
interaction
SRSF1
TUG1,
ACSL4
was
checked
via
RNA
pull-down/RIP
stability
assays.
LncRNA
TUG1
SRSF1/ACSL4-mediated
H/R-treated
HK-2
AKI
mouse
models.USC-Exo
treatment
improved
ameliorated
models.
rich
which
suppressed
exposed
to
H/R.
Mechanistically,
regulates
mRNA
interacting
with
RNA-binding
protein
SRSF1.
In
addition,
upregulation
or
downregulation
partially
reversed
cells.
Further,
TUG1's
repression
mice.Collectively,
carried
regulated
ASCL4-mediated
then
protected
AKI.
Potentially,
can
serve
as
a
promising
therapeutic
method
for
IRI-AKI.
Endocrine Reviews,
Год журнала:
2022,
Номер
43(3), С. 441 - 468
Опубликована: Апрель 21, 2022
Abstract
During
the
last
decade,
there
has
been
great
interest
in
elucidating
biological
role
of
extracellular
vesicles
(EVs),
particularly,
their
hormone-like
cell-to-cell
communication.
The
field
endocrinology
is
uniquely
placed
to
provide
insight
into
functions
EVs,
which
are
secreted
from
all
cells
fluids
and
carry
endocrine
signals
engage
paracellular
distal
interactions.
EVs
a
heterogeneous
population
membrane-bound
varying
size,
content,
bioactivity.
specifically
packaged
with
signaling
molecules,
including
lipids,
proteins,
nucleic
acids,
released
via
exocytosis
biofluid
compartments.
regulate
activity
both
proximal
target
cells,
translational
activity,
metabolism,
growth,
development.
As
such,
represents
an
integral
pathway
mediating
intercellular
Moreover,
as
content
cell-type
specific,
it
“fingerprint”
releasing
cell
its
metabolic
status.
Recently,
changes
profile
EV
bioactivity
have
described
several
endocrine-related
conditions
diabetes,
obesity,
cardiovascular
diseases,
cancer.
goal
this
statement
highlight
relevant
aspects
research
potential
endocrinology.
Extracellular
vesicles
(EVs)
are
transport
secreted
by
living
cells
and
released
into
the
extracellular
environment.
Recent
studies
have
shown
that
EVs
serve
as
"messengers"
in
intercellular
inter-organismal
communication,
both
normal
pathological
processes.
EVs,
natural
nanocarriers,
can
deliver
bioactivators
therapy
with
their
endogenous
properties.
This
review
article
describes
engineering
of
sources,
isolation
method,
cargo
loading,
boosting
approach,
adjustable
targeting
EVs.
Furthermore,
summarizes
recent
progress
made
EV-based
delivery
systems
applications,
including
cancer,
cardiovascular
diseases,
liver,
kidney,
nervous
system
COVID-19
emphasizes
obstacles
challenges
therapies
possible
strategies.
