Simultaneous Detection of Bladder Cancer Exosomal MicroRNAs Based on Inorganic Nanoflare and DNAzyme Walker DOI
Xiao Zhang, Xiaowei Wei,

Jijin Qi

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(11), P. 4787 - 4793

Published: March 11, 2022

Bladder cancer (BC) is one of the most common cancers in world, with high morbidity and mortality. It essential to develop a non-invasive, highly accurate, simple method for BC diagnosis. This work proposed fluorescent biosensor based on inorganic nanoflares combined DNAzyme walker simultaneous detection exosomal microRNAs (miRNAs). was constructed Au nanoparticle (AuNP) modified carbon dot (CD)-labeled substrates strands (AuNP@CDs nanoflares-DNAzyme, APCD). In presence target miRNAs, activated then cleaved CD-labeled automatically walked along AuNP, allowing fluorescence recovery. Due structure functional composition, APCD biosensors demonstrated sensitivity specificity, reached limit single miRNA at femtomolar level wide linear range from 50 fM 10 nM. Furthermore, analysis BC-related miR-133b miR-135b clinical serum specimens achieved consistent qRT-PCR, suggesting it potential diagnosis other cancers.

Language: Английский

Current Strategies for Exosome Cargo Loading and Targeting Delivery DOI Creative Commons
Haifeng Zeng,

Shaoshen Guo,

Xuancheng Ren

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(10), P. 1416 - 1416

Published: May 17, 2023

Extracellular vesicles (EVs) such as ectosomes and exosomes have gained attention promising natural carriers for drug delivery. Exosomes, which range from 30 to 100 nm in diameter, possess a lipid bilayer are secreted by various cells. Due their high biocompatibility, stability, low immunogenicity, favored cargo carriers. The membrane of also offers protection against degradation, making them desirable candidate However, loading into remains be challenge. Despite strategies incubation, electroporation, sonication, extrusion, freeze–thaw cycling, transfection that been developed facilitate loading, inadequate efficiency still persists. This review an overview current delivery using summarizes recent approaches small-molecule, nucleic acid, protein drugs exosomes. With insights these studies, we provide ideas more efficient effective molecules

Language: Английский

Citations

147
Extracellular vesicles mediate the communication of adipose tissue with brain and promote cognitive impairment associated with insulin resistance DOI Creative Commons
Jin Wang, Liang Li, Zhou Zhang

et al.

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(9), P. 1264 - 1279.e8

Published: Sept. 1, 2022

Language: Английский

Citations

130
Aberrant iron distribution via hepatocyte-stellate cell axis drives liver lipogenesis and fibrosis DOI Creative Commons
Hong Gao, Zhongmou Jin, Gautam Bandyopadhyay

et al.

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(8), P. 1201 - 1213.e5

Published: Aug. 1, 2022

Language: Английский

Citations

115
Inter-organ crosstalk during development and progression of type 2 diabetes mellitus DOI Open Access
Georgia Xourafa,

Melis Korbmacher,

Michael Roden

et al.

Nature Reviews Endocrinology, Journal Year: 2023, Volume and Issue: 20(1), P. 27 - 49

Published: Oct. 16, 2023

Language: Английский

Citations

92
Role of exosomes in the pathogenesis, diagnosis, and treatment of central nervous system diseases DOI Creative Commons
Yishu Fan, Zhuohui Chen, Mengqi Zhang

et al.

Journal of Translational Medicine, Journal Year: 2022, Volume and Issue: 20(1)

Published: June 27, 2022

Central nervous system (CNS) diseases, such as multiple sclerosis, Alzheimer's disease (AD), and Parkinson's (PD), affect millions of people around the world. Great efforts were put in related research, but few breakthroughs have been made diagnostic therapeutic approaches. Exosomes are cell-derived extracellular vesicles containing diverse biologically active molecules secreted by their cell origin. These contents, including nucleic acids, proteins, lipids, amino metabolites, can be transferred between different cells, tissues, or organs, regulating various intercellular cross-organ communications normal pathogenic processes. Considering that cellular environment state strongly impact content uptake efficiency exosomes, detection biological fluids composition analysis potentially offer a multicomponent readout several human diseases. Recently, studies found aberrant secretion exosomes closely to pathogenesis CNS Besides, loading natural cargoes, deliver drugs cross blood brain barrier, making them emerging candidates biomarkers therapeutics for In this review, we summarize discuss advanced research progress pathological processes diseases regarding with neuroinflammation, repair, protein aggregation. Moreover, propose strategies applying diagnosis, early detection, treatment

Language: Английский

Citations

91
MiR-690 treatment causes decreased fibrosis and steatosis and restores specific Kupffer cell functions in NASH DOI
Hong Gao, Zhongmou Jin, Gautam Bandyopadhyay

et al.

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(7), P. 978 - 990.e4

Published: June 13, 2022

Language: Английский

Citations

79
Exosomal lncRNA TUG1 derived from human urine-derived stem cells attenuates renal ischemia/reperfusion injury by interacting with SRSF1 to regulate ASCL4-mediated ferroptosis DOI Creative Commons
Zejia Sun, Jiyue Wu, Qing Bi

et al.

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: July 15, 2022

Human urine-derived stem cells (USCs)-derived exosomes (USC-Exo) could improve kidney ischemia/reperfusion injury (IRI), while the underlying mechanisms of this protective effect remain unclear.Human USCs and USC-Exo were isolated verified by morphology specific biomarkers. The effects on ferroptosis detected in IRI-induced acute (AKI) model C57BL/6 mice. lncRNA taurine-upregulated gene 1 (TUG1) hypoxia/reoxygenation (H/R)-treated human proximal tubular epithelial (HK-2). interaction SRSF1 TUG1, ACSL4 was checked via RNA pull-down/RIP stability assays. LncRNA TUG1 SRSF1/ACSL4-mediated H/R-treated HK-2 AKI mouse models.USC-Exo treatment improved ameliorated models. rich which suppressed exposed to H/R. Mechanistically, regulates mRNA interacting with RNA-binding protein SRSF1. In addition, upregulation or downregulation partially reversed cells. Further, TUG1's repression mice.Collectively, carried regulated ASCL4-mediated then protected AKI. Potentially, can serve as a promising therapeutic method for IRI-AKI.

Language: Английский

Citations

74
Extracellular Vesicles and Their Emerging Roles as Cellular Messengers in Endocrinology: An Endocrine Society Scientific Statement DOI Open Access
Carlos Salomón, Saumya Das,

Uta Erdbrügger

et al.

Endocrine Reviews, Journal Year: 2022, Volume and Issue: 43(3), P. 441 - 468

Published: April 21, 2022

Abstract During the last decade, there has been great interest in elucidating biological role of extracellular vesicles (EVs), particularly, their hormone-like cell-to-cell communication. The field endocrinology is uniquely placed to provide insight into functions EVs, which are secreted from all cells fluids and carry endocrine signals engage paracellular distal interactions. EVs a heterogeneous population membrane-bound varying size, content, bioactivity. specifically packaged with signaling molecules, including lipids, proteins, nucleic acids, released via exocytosis biofluid compartments. regulate activity both proximal target cells, translational activity, metabolism, growth, development. As such, represents an integral pathway mediating intercellular Moreover, as content cell-type specific, it “fingerprint” releasing cell its metabolic status. Recently, changes profile EV bioactivity have described several endocrine-related conditions diabetes, obesity, cardiovascular diseases, cancer. goal this statement highlight relevant aspects research potential endocrinology.

Language: Английский

Citations

73
Plant exosome nanovesicles (PENs): green delivery platforms DOI
Min Cao,

Ningning Diao,

Xiaolu Cai

et al.

Materials Horizons, Journal Year: 2023, Volume and Issue: 10(10), P. 3879 - 3894

Published: Jan. 1, 2023

Structure of plant-derived vesicles and their bioactive applications. Created with BioRender.com

Language: Английский

Citations

68
Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications DOI Creative Commons
Liwei Wang, Di Wang, Zhaoming Ye

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

Citations

62