Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream of Drosophila clock neurons DOI Creative Commons

Alison Ehrlich,

Audrey Xu,

Sofia Luminari

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

Summary Knowing how neural circuits change with neuronal plasticity and differ between individuals is important to fully understand behavior. Connectomes are typically assembled using electron microscopy, but this low throughput impractical for analyzing or mutations. Here, we modified the trans -Tango genetic circuit-tracing technique identify neurons synaptically downstream of Drosophila s-LNv clock neurons, which show 24hr rhythms. target were labeled specifically in adult flies a nuclear reporter gene, facilitated their purification then single cell sequencing. We call Tango-seq, it allows transcriptomic data – thus identity be overlayed on top anatomical data. found that s-LNvs preferentially make synaptic connections subset CNMa+ DN1p these likely plastic connections. also identified mushroom body Kenyon cells. Tango-seq should useful addition connectomics toolkit.

Language: Английский

Molecular mechanisms and physiological importance of circadian rhythms DOI
Alina Patke, Michael W. Young, Sofia Axelrod

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2019, Volume and Issue: 21(2), P. 67 - 84

Published: Nov. 25, 2019

Language: Английский

Citations

974
Circadian rhythms in neuronal activity propagate through output circuits DOI
Matthieu Cavey,

Ben Collins,

Claire Bertet

et al.

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(4), P. 587 - 595

Published: Feb. 29, 2016

Language: Английский

Citations

121
Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy DOI Creative Commons
Afroditi Petsakou, Themistoklis P. Sapsis, Justin Blau

et al.

Cell, Journal Year: 2015, Volume and Issue: 162(4), P. 823 - 835

Published: July 30, 2015

Language: Английский

Citations

95
Organization of Circadian Behavior Relies on Glycinergic Transmission DOI Creative Commons
Lía Frenkel, Nara I. Muraro, Andrea N. Beltrán González

et al.

Cell Reports, Journal Year: 2017, Volume and Issue: 19(1), P. 72 - 85

Published: April 1, 2017

Language: Английский

Citations

88
A Serotonin-Modulated Circuit Controls Sleep Architecture to Regulate Cognitive Function Independent of Total Sleep in Drosophila DOI Creative Commons
Chang Liu, Zhiqiang Meng, Timothy D. Wiggin

et al.

Current Biology, Journal Year: 2019, Volume and Issue: 29(21), P. 3635 - 3646.e5

Published: Oct. 24, 2019

Both the structure and amount of sleep are important for brain function. Entry into deep, restorative stages is time dependent; short bouts selectively eliminate these states. Fragmentation-induced cognitive dysfunction a feature many common human pathologies. Whether normally regulated independent unknown. Here, we show that in Drosophila melanogaster, activation subset serotonergic neurons fragments without major changes total sleep, dramatically reducing long episodes may correspond to deep Disruption results learning deficits can be rescued by pharmacologically or genetically consolidating sleep. We identify two reciprocally connected sets ellipsoid body form heart serotonin-modulated circuit controls architecture. Taken together, findings define essential controlling its amount.

Language: Английский

Citations

83
Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity DOI Creative Commons
Matthias Schlichting, Madelen M. Díaz,

Jason Xin

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Oct. 15, 2019

Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian clocks continue to tick away without intercellular communication. To investigate issues Drosophila, we assayed behavior as well molecular within individual brain clock neurons while blocking communication the ca. 150 neuron network. We also generated CRISPR-mediated neuron-specific knockouts. The results point two key groups: loss of both regions but neither one alone has a strong behavioral phenotype darkness; between contributes period determination. Under dark conditions, region persists network famous PDF-expressing s-LNv however was strongly dependent on communication, likely because gene expression vulnerable sLNvs depends neuronal firing or light.

Language: Английский

Citations

79
Mutual coupling of neurons in the circadian master clock: what we can learn from fruit flies DOI Creative Commons
Charlotte Helfrich‐Förster, Nils Reinhard

Neurobiology of Sleep and Circadian Rhythms, Journal Year: 2025, Volume and Issue: 18, P. 100112 - 100112

Published: Jan. 18, 2025

Circadian master clocks in the brain consist of multiple neurons that are organized into populations with different morphology, physiology, and neuromessenger content presumably functions. In most animals, these distributed bilaterally, located close proximity to visual system, synchronized by eyes light-dark cycles environment. mammals cockroaches, each two consists a core region receives information from shell which output projections originate, whereas flies several other insects, lateral dorsal regions. all cases, morning evening clock seem exist, communication between them neurons, as well connection across hemispheres, is prerequisite for normal rhythmic function. Phenomena such rhythm splitting, internal desynchronization caused "decoupling" hemispheres or decoupling certain within one hemisphere. Since contain relatively few characterized at individual level, fly particularly suited study neurons. Here, we review organization bilateral brain, focus on synaptic paracrine connections comparison insects mammals.

Language: Английский

Citations

1
Synchrony and desynchrony in circadian clocks: impacts on learning and memory DOI Open Access

Harini C. Krishnan,

Lisa C. Lyons

Learning & Memory, Journal Year: 2015, Volume and Issue: 22(9), P. 426 - 437

Published: Aug. 18, 2015

Circadian clocks evolved under conditions of environmental variation, primarily alternating light dark cycles, to enable organisms anticipate daily events and coordinate metabolic, physiological, behavioral activities. However, modern lifestyle advances in technology have increased the percentage individuals working phases misaligned with natural circadian activity rhythms. Endogenous oscillators modulate alertness, acquisition learning, memory formation, recall examples modulation observed across phyla from invertebrates humans. Cognitive performance are significantly diminished when occurring out phase Disruptions regulation can lead impairment formation memories manifestation other cognitive deficits. This review explores types interactions through which clock modulates cognition, highlights recent progress identifying mechanistic between system processes involved outlines methods used remediate perturbations reinforce adaptation.

Language: Английский

Citations

88
Neuroanatomical details of the lateral neurons of Drosophila melanogaster support their functional role in the circadian system DOI Creative Commons
Frank K. Schubert,

Nicolas Hagedorn,

Taishi Yoshii

et al.

The Journal of Comparative Neurology, Journal Year: 2018, Volume and Issue: 526(7), P. 1209 - 1231

Published: Feb. 9, 2018

Drosophila melanogaster is a long-standing model organism in the circadian clock research. A major advantage relative small number of about 150 neurons, which built Drosophila. In our recent work, we focused on neuroanatomical properties lateral neurons network. By applying multicolor-labeling technique Flybow were able to identify anatomical similarity previously described E2 subunit evening oscillator clock, by 5th ventrolateral neuron (5th s-LNv ) and one ITP positive dorsolateral (LNd ). These two share same spatial functional properties. We found both innervating brain areas with similar pre- postsynaptic sites brain. Here findings support their shared function as main network like also previous studies. second quite surprising finding addresses large ventral PDF-neurons (l-LNv s). could show that four hardly distinguishable l-LNv s consist subgroups different innervation patterns. While three reflect well-known branching pattern reproduced PDF immunohistochemistry, per hemisphere has distinguished profile restricted only proximal part medulla-surface. named this "extra" x). suggest subgroups.

Language: Английский

Citations

79
Reconfiguration of a Multi-oscillator Network by Light in the Drosophila Circadian Clock DOI Creative Commons
Abhishek Chatterjee, Angélique Lamaze,

Joydeep De

et al.

Current Biology, Journal Year: 2018, Volume and Issue: 28(13), P. 2007 - 2017.e4

Published: June 14, 2018

Language: Английский

Citations

76