An ammonium transporter is a non-canonical olfactory receptor for ammonia

Current Biology, Год журнала: 2021, Номер 31(15), С. 3382 - 3390.e7

Опубликована: Июнь 9, 2021

Язык: Английский

---

Transsynaptic mapping of Drosophila mushroom body output neurons

eLife, Год журнала: 2021, Номер 10

Опубликована: Фев. 10, 2021

The mushroom body (MB) is a well-characterized associative memory structure within the

Язык: Английский

---

Functional connectomics spanning multiple areas of mouse visual cortex

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Июль 29, 2021

Abstract To understand the brain we must relate neurons’ functional responses to circuit architecture that shapes them. Here, present a large connectomics dataset with dense calcium imaging of millimeter scale volume. We recorded activity from approximately 75,000 neurons in primary visual cortex (VISp) and three higher areas (VISrl, VISal VISlm) an awake mouse viewing natural movies synthetic stimuli. The data were co-registered volumetric electron microscopy (EM) reconstruction containing more than 200,000 cells 0.5 billion synapses. Subsequent proofreading subset this volume yielded reconstructions include complete dendritic trees as well local inter-areal axonal projections map up thousands cell-to-cell connections per neuron. release open-access resource scientific community including set tools facilitate retrieval downstream analysis. In accompanying papers describe our findings using provide comprehensive structural characterization cortical cell types 1–3 most detailed synaptic level connectivity diagram column date 2 , uncovering unique cell-type specific inhibitory motifs can be linked gene expression 4 . Functionally, identify new computational principles how information is integrated across space 5 characterize novel neuronal invariances 6 bring structure function together decipher general principle wires excitatory within 7, 8

Язык: Английский

---

Dopamine modulation of sensory processing and adaptive behavior in flies

Cell and Tissue Research, Год журнала: 2021, Номер 383(1), С. 207 - 225

Опубликована: Янв. 1, 2021

Abstract Behavioral flexibility for appropriate action selection is an advantage when animals are faced with decisions that will determine their survival or death. In order to arrive at the right decision, evaluate information from external environment, internal state, and past experiences. How these different signals integrated modulated in brain, how context- state-dependent behavioral controlled poorly understood questions. Studying molecules help convey integrate such neural circuits important way approach Many years of work model organisms have shown dopamine a critical neuromodulator (reward based) associative learning. However, recent findings vertebrates invertebrates demonstrated complexity heterogeneity dopaminergic neuron populations functional implications many adaptive behaviors survival. For example, neurons can sensory information, states, learned experience decision making circuitry. Several advances methodologies availability synaptic level connectome whole-brain circuitry Drosophila melanogaster make fly attractive system study roles behavior. particular, learning memory center—the mushroom body—is richly innervated by enable it multi-modal according state context, modulate decision-making

Язык: Английский

---

The Neuronal Circuit of the Dorsal Circadian Clock Neurons in Drosophila melanogaster

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 29, 2022

Drosophila's dorsal clock neurons (DNs) consist of four clusters (DN1as, DN1ps, DN2s, and DN3s) that largely differ in size. While the DN1as DN2s encompass only two neurons, DN1ps ∼15 DN3s comprise ∼40 per brain hemisphere. In comparison to well-characterized lateral (LNs), neuroanatomy function DNs are still not clear. Over past decade, numerous studies have addressed their role fly's circadian system, leading several sometimes divergent results. Nonetheless, these agreed important fine-tune activity under light temperature cycles play essential roles linking output from LNs downstream control sleep metabolism. Here, we used Flybow specific split-GAL4 lines, trans-Tango, recently published fly connectome (called hemibrain) describe morphology greater detail, including synaptic connections other non-clock neurons. We show some DN groups heterogenous. certain strongly connected with LNs, others mainly signal circuits clock. Among latter mushroom body central complex tubercle bulb neurosecretory cells pars intercerebralis, unidentified partners. This heterogeneity may explain conflicting results previously found about functionality. Most importantly, identify putative novel communication centers network: one fiber bundle superior protocerebrum running toward anterior optic hub posterior protocerebrum. Both invaded by might an instrumental network.

Язык: Английский

---