Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance DOI Creative Commons
Christine A. Tabuloc, Curtis R. Carlson, Fatemeh Ganjisaffar

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 27, 2024

Abstract Drosophila suzukii lay eggs in soft-skinned, ripening fruits, making this insect a serious threat to berry production. Since its 2008 introduction into North America, growers have used insecticides, such as pyrethroids and spinosads, the primary approach for D. management, resulting development of insecticide resistance pest. This study sought identify molecular mechanisms conferring these populations. We sequenced transcriptomes two pyrethroid- spinosad-resistant isofemale lines. In both pyrethroid-resistant lines one line, we identified overexpression metabolic genes that are implicated other pests. observed an cuticular been linked resistance. Our findings enabled diagnostics confirm persistence California, U.S.A. To validate findings, leveraged melanogaster mutants with reduced expression or were found be upregulated resistant demonstrate involved promoting is first characterize provides insights how current management practices can optimized.

Язык: Английский

Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions DOI Creative Commons
Dick R. Nässel, Meet Zandawala

Open Biology, Год журнала: 2022, Номер 12(7)

Опубликована: Июль 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Язык: Английский

Процитировано

57
Neural Circuits Underlying Behavioral Flexibility: Insights From Drosophila DOI Creative Commons
Anita V. Devineni, Kristin M. Scaplen

Frontiers in Behavioral Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Янв. 6, 2022

Behavioral flexibility is critical to survival. Animals must adapt their behavioral responses based on changes in the environmental context, internal state, or experience. Studies Drosophila melanogaster have provided insight into neural circuit mechanisms underlying flexibility. Here we discuss how behavior modulated by and learning. We describe general principles of organization modulation that underlie flexibility, are likely extend other species.

Язык: Английский

Процитировано

41
Unveiling the sensory and interneuronal pathways of the neuroendocrine connectome in Drosophila DOI Creative Commons
Sebastian Hückesfeld, Philipp Schlegel, Anton Miroschnikow

и другие.

eLife, Год журнала: 2021, Номер 10

Опубликована: Июнь 2, 2021

Neuroendocrine systems in animals maintain organismal homeostasis and regulate stress response. Although a great deal of work has been done on the neuropeptides hormones that are released act target organs periphery, synaptic inputs onto these neuroendocrine outputs brain less well understood. Here, we use transmission electron microscopy reconstruction whole central nervous system Drosophila larva to elucidate sensory pathways interneurons provide input neurosecretory cells projecting endocrine organs. Predicted by network modeling, also identify new carbon dioxide-responsive acts specific set includes those expressing corazonin (Crz) diuretic hormone 44 (Dh44) neuropeptides. Our analysis reveals neuronal architecture for combinatorial action based interneuronal converge distinct combinations outputs.

Язык: Английский

Процитировано

44
The neuronal logic of how internal states control food choice DOI
Daniel Münch, Dennis Goldschmidt, Carlos Ribeiro

и другие.

Nature, Год журнала: 2022, Номер 607(7920), С. 747 - 755

Опубликована: Июль 6, 2022

Язык: Английский

Процитировано

34
Exploring the role of gut microbiota in host feeding behavior among breeds in swine DOI Creative Commons
Yuqing He, Francesco Tiezzi, Jeremy Howard

и другие.

BMC Microbiology, Год журнала: 2022, Номер 22(1)

Опубликована: Янв. 3, 2022

Abstract Background The interplay between the gut microbiota and feeding behavior has consequences for host metabolism health. present study aimed to explore overall influence on traits identify specific microbes associated with in three commercial swine breeds at growth stages. Feeding measures were obtained from 651 pigs of (Duroc, Landrace, Large White) an average 73 163 days age. Seven covered information feed intake, feeder occupation time, rate, number visits feeder. Rectal swabs collected each pig ± 3, 123 4, 158 4 DNA was extracted subjected 16 S rRNA gene sequencing. Results Differences among during period found. proportion phenotypic variances explained by microbial composition small moderate (ranged 0.09 0.31). A total 21, 10, 35 amplicon sequence variants found be significantly (q-value < 0.05) Duroc, White across sampling time points. identified annotated five phyla, Firmicutes being most abundant. Those assigned 28 genera, mainly including Christensenellaceae _R-7_group, Ruminococcaceae _UCG-004, Dorea, _UCG-014, Marvinbryantia . Conclusions This demonstrated importance interacting multiple archaea bacteria either or Our provides insight into interaction highlights genetic background age effects studies.

Язык: Английский

Процитировано

29
Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis in Drosophila DOI Open Access
Jayati Gera, Marishia Agard,

Hannah Nave

и другие.

Опубликована: Май 7, 2024

Insects have evolved a variety of neurohormones that enable them to maintain their nutrient and osmotic homeostasis. While the identities functions various insect metabolic diuretic hormones been well-established, characterization an anti-diuretic signaling system is conserved across most insects still lacking. To address this, here we characterized ion transport peptide (ITP) in Drosophila . The ITP gene encodes five transcript variants which generate three different isoforms: amidated (ITPa) two ITP-like (ITPL1 ITPL2) isoforms. Using combination anatomical mapping single-cell transcriptome analyses, comprehensively expression all isoforms nervous peripheral tissues. Our analyses reveal widespread Moreover, show ITPa released during dehydration recombinant inhibits peptide-induced renal tubule secretion ex vivo , thus confirming its role as hormone. phylogenetic-driven approach assay, identified functionally Gyc76C, membrane guanylate cyclase, elusive receptor. Thus, knockdown Gyc76C tubules abolishes inhibitory effect on hormone secretion. Extensive reveals it highly expressed larval adult tissues associated with osmoregulation (renal rectum) homeostasis (fat body). Consistent this expression, impacts tolerance ionic stresses, whereas specifically fat body feeding, behaviors. We also complement receptor experiments overexpression neurons. Interestingly, ITPa-Gyc76C pathways deciphered are reminiscent atrial natriuretic mammals. Lastly, utilized connectomics transcriptomics identify synaptic paracrine upstream downstream ITP-expressing Taken together, our systematic establishes tractable decipher how small set neurons integrates diverse inputs orchestrate systemic

Язык: Английский

Процитировано

6
Cholecystokinin-like peptide mediates satiety by inhibiting sugar attraction DOI Creative Commons
Di Guo, Yijie Zhang, Su Zhang

и другие.

PLoS Genetics, Год журнала: 2021, Номер 17(8), С. e1009724 - e1009724

Опубликована: Авг. 16, 2021

Feeding is essential for animal survival and reproduction regulated by both internal states external stimuli. However, little known about how influence the perception of sensory cues that regulate feeding behavior. Here, we investigated neuronal molecular mechanisms behind nutritional state-mediated regulation gustatory in control behavior brown planthopper Drosophila. We found increases expression cholecystokinin-like peptide, sulfakinin (SK), activity a set SK-expressing neurons. Starvation elevates transcription sugar receptor Gr64f SK negatively regulates insects. Interestingly, one two receptors, CCKLR-17D3, expressed some Gr64f-expressing neurons proboscis proleg tarsi. Thus, have identified as neuropeptide signal circuitry responds to food intake, diminishing gene sweet sensing GRNs. Our findings demonstrate state-dependent pathway modulates thereby behavior, but our experiments cannot exclude further parallel pathways. Importantly, show underlying are conserved distantly related insect species.

Язык: Английский

Процитировано

27
Serotonergic modulation of swallowing in a complete fly vagus nerve connectome DOI Creative Commons
Andreas Schoofs, Anton Miroschnikow, Philipp Schlegel

и другие.

Current Biology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

4
Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis inDrosophila DOI Creative Commons
Jayati Gera, Marishia Agard,

Hannah Nave

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 10, 2024

Insects have evolved a variety of neurohormones that enable them to maintain their nutrient and osmotic homeostasis. While the identities functions various insect metabolic diuretic hormones been well-established, characterization an anti-diuretic signaling system is conserved across most insects still lacking. To address this, here we characterized ion transport peptide (ITP) in Drosophila . The ITP gene encodes five transcript variants which generate three different isoforms: amidated (ITPa) two ITP-like (ITPL1 ITPL2) isoforms. Using combination anatomical mapping single-cell transcriptome analyses, comprehensively expression all isoforms nervous peripheral tissues. Our analyses reveal widespread Moreover, show ITPa released during dehydration recombinant inhibits peptide-induced renal tubule secretion ex vivo , thus confirming its role as hormone. phylogenetic-driven approach assay, identified functionally Gyc76C, membrane guanylate cyclase, elusive receptor. Thus, knockdown Gyc76C tubules abolishes inhibitory effect on hormone secretion. Extensive reveals it highly expressed larval adult tissues associated with osmoregulation (renal rectum) homeostasis (fat body). Consistent this expression, impacts tolerance ionic stresses, whereas specifically fat body feeding, behaviors. We also complement receptor experiments overexpression neurons. Interestingly, ITPa-Gyc76C pathways deciphered are reminiscent atrial natriuretic mammals. Lastly, utilized connectomics transcriptomics identify synaptic paracrine upstream downstream ITP-expressing Taken together, our systematic establishes tractable decipher how small set neurons integrates diverse inputs orchestrate systemic

Язык: Английский

Процитировано

3
A Neuropeptide Signaling Network That Regulates Developmental Timing and Systemic Growth in Drosophila DOI
Yuya Ohhara,

Mikkal Blick,

Donghyun Park

и другие.

The Journal of Comparative Neurology, Год журнала: 2024, Номер 532(10)

Опубликована: Окт. 1, 2024

ABSTRACT Animals sense chemical cues such as nutritious and noxious stimuli through the chemosensory system adapt their behavior, physiology, developmental schedule to environment. In Drosophila central nervous system, interneurons that produce neuropeptides called Hugin (Hug) peptides receive signals from gustatory receptor neurons regulate feeding behavior. Because Hug project axons higher brain region within protocerebrum where dendrites of multiple producing developmentally important are extended, it has been postulated development neuroendocrine system. this study, we show interact with a subset prothoracicotropic hormone (PTTH) onset metamorphosis systemic growth. Loss hug gene silencing caused delay in larval‐to‐prepupal transition an increase final body size. Furthermore, deletion receptor‐encoding genes also size increase, phenotype was restored by expressing receptors PTTH‐producing neurons. These results indicate timing via This study provides basis for understanding how chemosensation is converted into signaling control insect growth development.

Язык: Английский

Процитировано

3