Microbiology Spectrum,
Год журнала:
2024,
Номер
12(4)
Опубликована: Фев. 20, 2024
ABSTRACT
Given
the
burgeoning
Nyctereutes
procyonoides
breeding
industry
and
its
growing
scale,
it
is
imperative
to
investigate
impact
of
high-fat
diets
on
health
these
animals.
This
study
involved
30
male
comparable
weights
(3
kg
±0.5),
randomly
assigned
either
a
control
group
or
diet
(
n
=
15
each).
The
latter
was
fed
mixture
lard
basal
in
2:5
ratio,
establishing
model
procyonoides.
induced
diarrhea
histopathological
changes
Analysis
small
intestine
contents
using
16S
rRNA
sequencing
revealed
diet-induced
disruption
gut
microbiota.
Specifically,
Escherichia-Shigella
emerged
as
biomarker
P
0.049),
while
Vagococcus
prevalent
indicating
significant
increase
harmful
bacteria
group.
Furthermore,
this
disrupted
flora
correlated
with
inflammation
oxidative
stress,
evidenced
by
marked
increases
TNF-α
<
0.01),
IL-1β
0.05),
IL-6
0.05)
levels,
measured
via
q-PCR,
Western
blot,
stress
assays.
In
addition,
q-PCR
analysis
upregulation
apoptosis
necrosis
markers,
including
Bax,
Caspase3,
Caspase9,
Caspase12,
RIPK3,
RIPK1
0.01
0.001),
concurrent
downregulation
anti-apoptotic
gene
Bcl-2
0.01)
group,
consistent
protein
expression
trends.
These
findings
suggest
that
alters
microbiome
toward
more
bacterial
composition,
escalating
inflammatory
responses
intestinal
tissue
permeability,
culminating
cell
necrosis.
IMPORTANCE
examines
Our
research
established
diet,
revealing
impacts,
such
diarrhea,
histological
anomalies,
alterations
emphasize
importance
preventing
issues
promoting
sustainable
growth.
They
highlight
microbiota
overall
animal
health.
Food & Function,
Год журнала:
2024,
Номер
15(7), С. 3411 - 3419
Опубликована: Янв. 1, 2024
Tetrabromobisphenol
A
(TBBPA)
is
a
global
pollutant.
When
TBBPA
absorbed
by
the
body
through
various
routes,
it
can
have
wide
range
of
harmful
effects
on
body.
Green
tea
polyphenols
(GTPs)
act
as
antioxidants,
resisting
toxic
animals.
The
and
mechanisms
GTP
oxidative
stress,
inflammation
apoptosis
in
mouse
lung
are
unknown.
Therefore,
we
established
vivo
vitro
models
exposure
antagonism
using
C57
mice
A549
cells
examined
expression
factors
related
to
autophagy,
apoptosis.
results
study
showed
that
increase
reactive
oxygen
species
(ROS)
levels
after
decreased
autophagy-related
Beclin1,
LC3-II,
ATG3,
ATG5,
ATG7
ATG12
increased
p62;
stress
inhibits
autophagy
levels.
pro-inflammatory
IL-1β,
IL-6
TNF-α
anti-inflammatory
factor
IL-10
activation
NF-κB
p65/TNF-α
pathway.
Bax,
caspase-3,
caspase-7
caspase-9
Bcl-2
activate
apoptosis-related
pathways.
addition
attenuated
levels,
restored
inhibition
reduced
Our
suggest
attenuate
modulating
ROS,
reducing
increasing
attenuating
cells.
These
provide
fundamental
information
for
exploring
antioxidant
mechanism
further
studying
TBBPA.
