Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Июнь 8, 2023
Extracellular
vesicles
(EVs)
have
emerged
as
a
promising
platform
for
gene
delivery
owing
to
their
natural
properties
and
phenomenal
functions,
being
able
circumvent
the
significant
challenges
associated
with
toxicity,
problematic
biocompatibility,
immunogenicity
of
standard
approaches.
These
features
are
particularly
interest
targeted
emerging
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/CRISPR-associated
(Cas)
systems.
However,
current
efficiency
EV-meditated
transport
CRISPR/Cas
components
remains
insufficient
due
numerous
exogenous
endogenous
barriers.
Here,
we
comprehensively
reviewed
status
EV-based
In
particular,
explored
various
strategies
methodologies
available
potentially
improve
loading
capacity,
safety,
stability,
targeting,
tracking
system
delivery.
Additionally,
hypothesise
future
avenues
development
systems
that
could
pave
way
novel
clinically
valuable
approaches,
may
bridge
gap
between
editing
technologies
laboratory/clinical
application
therapies.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Фев. 21, 2024
Abstract
Inflammation-associated
diseases
encompass
a
range
of
infectious
and
non-infectious
inflammatory
diseases,
which
continuously
pose
one
the
most
serious
threats
to
human
health,
attributed
factors
such
as
emergence
new
pathogens,
increasing
drug
resistance,
changes
in
living
environments
lifestyles,
aging
population.
Despite
rapid
advancements
mechanistic
research
development
for
these
current
treatments
often
have
limited
efficacy
notable
side
effects,
necessitating
more
effective
targeted
anti-inflammatory
therapies.
In
recent
years,
nanotechnology
has
provided
crucial
technological
support
prevention,
treatment,
detection
inflammation-associated
diseases.
Various
types
nanoparticles
(NPs)
play
significant
roles,
serving
vaccine
vehicles
enhance
immunogenicity
carriers
improve
targeting
bioavailability.
NPs
can
also
directly
combat
pathogens
inflammation.
addition,
facilitated
biosensors
pathogen
imaging
techniques
This
review
categorizes
characterizes
different
NPs,
summarizes
their
applications
It
discusses
challenges
associated
with
clinical
translation
this
field
explores
latest
developments
prospects.
conclusion,
opens
up
possibilities
comprehensive
management
Journal of Nanobiotechnology,
Год журнала:
2022,
Номер
20(1)
Опубликована: Дек. 27, 2022
Abstract
Synthetic
nanoparticles
with
surface
bioconjugation
are
promising
platforms
for
targeted
therapy,
but
their
simple
biological
functionalization
is
still
a
challenging
task
against
the
complex
intercellular
environment.
Once
synthetic
enter
body,
they
phagocytosed
by
immune
cells
system.
Recently,
cell
membrane
camouflage
strategy
has
emerged
as
novel
therapeutic
tactic
to
overcome
these
issues
utilizing
fundamental
properties
of
natural
cells.
Macrophage,
type
system
cells,
plays
critical
roles
in
various
diseases,
including
cancer,
atherosclerosis,
rheumatoid
arthritis,
infection
and
inflammation,
due
recognition
engulfment
function
removing
substances
pathogens.
Macrophage
membranes
inherit
protein
profiles
biointerfacing
source
Therefore,
macrophage
cloaking
can
protect
from
phagocytosis
Meanwhile,
make
use
correspondence
accurately
recognize
antigens
target
inflamed
tissue
or
tumor
sites.
In
this
review,
we
have
summarized
advances
fabrication,
characterization
homing
capacity
cancers,
cardiovascular
central
nervous
microbial
infections.
Although
membrane-camouflaged
currently
fetal
stage
development,
there
huge
potential
challenge
explore
conversion
mode
clinic.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 571 - 608
Опубликована: Янв. 1, 2024
Abstract:
With
the
development
of
nanotechnology,
nanoparticles
(NPs)
have
shown
broad
prospects
as
drug
delivery
vehicles.
However,
they
exhibit
certain
limitations,
including
low
biocompatibility,
poor
physiological
stability,
rapid
clearance
from
body,
and
nonspecific
targeting,
which
hampered
their
clinical
application.
Therefore,
novel
systems
with
improved
biocompatibility
high
target
specificity
remains
a
major
challenge.
In
recent
years,
biofilm
mediated
biomimetic
nano-drug
system
(BNDDS)
has
become
research
hotspot
focus
in
field
life
sciences.
This
new
platform
uses
bio-nanotechnology
to
encapsulate
synthetic
NPswithin
membrane,
organically
integrating
immunogenicity,
toxicity,
tumor
good
adjustability
versatility
nanocarrier,
shows
promising
applications
precision
therapy.
this
review,
we
systematically
summarize
progress
BNDDS
used
for
optimizing
delivery,
providing
theoretical
reference
designing
safe
efficient
treatment
strategies
improve
outcomes.
Keywords:
nanoparticles,
cell
targeted
therapy
Bioactive Materials,
Год журнала:
2024,
Номер
36, С. 126 - 156
Опубликована: Март 2, 2024
Small
extracellular
vesicles
(sEVs)
are
known
to
be
secreted
by
a
vast
majority
of
cells.
These
sEVs,
specifically
exosomes,
induce
specific
cell-to-cell
interactions
and
can
activate
signaling
pathways
in
recipient
cells
through
fusion
or
interaction.
nanovesicles
possess
several
desirable
properties,
making
them
ideal
for
regenerative
medicine
nanomedicine
applications.
properties
include
exceptional
stability,
biocompatibility,
wide
biodistribution,
minimal
immunogenicity.
However,
the
practical
utilization
particularly
clinical
settings
at
large
scale,
is
hindered
expensive
procedures
required
their
isolation,
limited
circulation
lifetime,
suboptimal
targeting
capacity.
Despite
these
challenges,
sEVs
have
demonstrated
remarkable
ability
accommodate
various
cargoes
found
extensive
applications
biomedical
sciences.
To
overcome
limitations
broaden
potential
applications,
researchers
should
strive
deepen
understanding
current
loading,
characterization
techniques.
Additionally,
acquiring
fundamental
knowledge
about
origins
employing
state-of-the-art
methodologies
expand
research
scope.
This
review
provides
comprehensive
overview
exosome-based
strategies
diverse
domains,
encompassing
cancer
therapy,
immunotherapy,
biomarker
Furthermore,
we
emphasize
immense
exosomes
medicine.
ACS Nano,
Год журнала:
2024,
Номер
18(4), С. 2782 - 2799
Опубликована: Янв. 17, 2024
Immune
regulation
therapies
are
considered
promising
for
treating
classically
activated
macrophage
(M1)-driven
viral
myocarditis
(VM).
Alternatively,
(M2)-derived
extracellular
vesicles
(M2
EVs)
have
great
immunomodulatory
potential
owing
to
their
ability
reprogram
macrophages,
but
therapeutic
efficacy
is
hampered
by
insufficient
targeting
capacity
in
vivo.
Therefore,
we
developed
cardiac-targeting
peptide
(CTP)
and
platelet
membrane
(PM)-engineered
M2
EVs
enriched
with
inflammatory
protein-II
(vMIP-II),
termed
CTP/PM-M2
EVsvMIP-II-Lamp2b,
improve
the
delivery
of
"cargo"
heart
tissues.
In
a
mouse
model
VM,
intravenously
injected
EVsvMIP-II-Lamp2b
could
be
carried
into
myocardium
via
CTP,
PM,
vMIP-II.
microenvironment,
macrophages
differentiated
from
circulating
monocytes
residing
showed
enhanced
endocytosis
rates
EVsvMIP-II-Lamp2b.
Subsequently,
successfully
released
functional
cytosol,
which
facilitated
reprogramming
M1
reparative
macrophages.
vMIP-II
not
only
helps
increase
also
collaborates
regulate
microenvironment
downregulate
levels
multiple
chemokine
receptors.
Finally,
cardiac
immune
was
protectively
regulated
achieve
repair.
Taken
together,
our
findings
suggest
that
CTP-and-PM-engineered
represent
an
effective
means
VM
show
promise
clinical
applications.
Frontiers in Immunology,
Год журнала:
2021,
Номер
12
Опубликована: Дек. 8, 2021
Immune
cell-derived
extracellular
vesicles
(EVs)
have
increasingly
become
the
focus
of
research
due
to
their
unique
characteristics
and
bioinspired
applications.
They
are
lipid
bilayer
membrane
nanosized
harboring
a
range
immune
surface
receptors
effector
molecules
from
parental
cells.
EVs
important
mediators
intercellular
communication
that
regulate
specific
mechanisms
adaptive
innate
responses.
However,
underlying
antitumor
effects
still
being
explored.
Importantly,
some
features,
including
accessibility,
storage,
ability
pass
through
blood-brain
blood-tumor
barriers,
loading
various
molecules.
been
directly
applied
or
engineered
as
potent
vaccines
for
diagnosis
clinical
diseases.
More
applications
involving
genetic
engineering,
cargo
delivery
strategies
improved
treatment
efficacy
EVs.
EV-based
therapies
expected
separate
technique
complement
immunotherapy,
radiotherapy,
chemotherapy
other
therapeutic
modalities.
This
review
aims
provide
comprehensive
overview
functions
derived
(CD4+
T,
CD8+
T
B
cells)
cells
(macrophages,
NK
cells,
DCs,
neutrophils)
discuss
emerging
opportunities
prospects
in
cancer
treatment.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4333 - 4333
Опубликована: Фев. 22, 2023
Respiratory
diseases,
such
as
asthma
and
chronic
obstructive
pulmonary
disease
(COPD),
are
critical
areas
of
medical
research,
millions
people
affected
worldwide.
In
fact,
more
than
9
million
deaths
worldwide
were
associated
with
respiratory
diseases
in
2016,
equivalent
to
15%
global
deaths,
the
prevalence
is
increasing
every
year
population
ages.
Due
inadequate
treatment
options,
treatments
for
many
limited
relieving
symptoms
rather
curing
disease.
Therefore,
new
therapeutic
strategies
urgently
needed.
Poly
(lactic-co-glycolic
acid)
micro/nanoparticles
(PLGA
M/NPs)
have
good
biocompatibility,
biodegradability
unique
physical
chemical
properties,
making
them
one
most
popular
effective
drug
delivery
polymers.
this
review,
we
summarized
synthesis
modification
methods
PLGA
M/NPs
their
applications
(asthma,
COPD,
cystic
fibrosis
(CF),
etc.)
also
discussed
research
progress
current
status
diseases.
It
was
concluded
that
promising
vehicles
due
advantages
low
toxicity,
high
bioavailability,
loading
capacity,
plasticity
modifiability.
And
at
end,
presented
an
outlook
on
future
directions,
aiming
provide
some
ideas
directions
hopefully
promote
widespread
application
clinical
treatment.
