Clinical and Translational Medicine,
Год журнала:
2024,
Номер
14(4)
Опубликована: Апрель 1, 2024
Breast
cancer
remains
a
global
health
challenge,
necessitating
innovative
therapeutic
approaches.
Immunomodulation
and
immunotherapy
have
emerged
as
promising
strategies
for
breast
treatment.
Engineered
exosomes
are
the
sort
of
modified
with
surface
decoration
internal
molecules.
Through
suitable
modifications,
engineered
exhibit
capability
to
overcome
limitations
associated
traditional
This
ability
opens
up
novel
avenues
development
more
effective,
personalized,
minimally
invasive
interventions.
Theranostics,
Год журнала:
2024,
Номер
14(5), С. 2099 - 2126
Опубликована: Янв. 1, 2024
Exosomes,
which
are
small
vesicles
enclosed
by
a
lipid
bilayer
and
released
many
cell
types,
widely
dispersed
have
garnered
increased
attention
in
the
field
of
regenerative
medicine
due
to
their
ability
serve
as
indicators
diseases
agents
with
therapeutic
potential.
Exosomes
play
crucial
role
mediating
intercellular
communication
through
transfer
biomolecules,
including
proteins,
lipids,
RNA,
other
molecular
constituents,
between
cells.
The
targeted
transport
proteins
nucleic
acids
specific
cells
has
potential
enhance
or
impair
biological
functions.
applications,
they
can
be
used
alone
combination
approaches.
examination
unique
attributes
functions
these
factors
emerged
prominent
study
realm
biomedical
research.
This
manuscript
summarizes
origins
properties
exosomes,
structural,
biological,
physical,
chemical
aspects.
paper
offers
complete
recent
progress
tissue
repair
medicine,
emphasizing
possible
implications
methods
forthcoming
regeneration
attempts.
ACS Nano,
Год журнала:
2024,
Номер
18(32), С. 21091 - 21111
Опубликована: Авг. 5, 2024
Milk
exosomes
(mExos)
have
demonstrated
significant
promise
as
vehicles
for
the
oral
administration
of
protein
and
peptide
drugs
owing
to
their
superior
capacity
traverse
epithelial
barriers.
Nevertheless,
certain
challenges
persist
due
intrinsic
characteristics,
including
suboptimal
drug
loading
efficiency,
inadequate
mucus
penetration
capability,
susceptibility
membrane
loss.
Herein,
a
hybrid
vesicle
with
self-adaptive
surface
properties
(mExos@DSPE-Hyd-PMPC)
was
designed
by
fusing
functionalized
liposomes
natural
mExos,
aiming
overcome
limitations
associated
mExos
unlock
full
potential
in
delivery.
The
property
transformation
mExos@DSPE-Hyd-PMPC
achieved
introducing
pH-sensitive
hydrazone
bond
between
highly
hydrophilic
zwitterionic
polymer
phospholipids,
utilizing
pH
microenvironment
on
jejunum
surface.
In
comparison
vesicles
exhibited
2.4-fold
enhancement
encapsulation
efficiency
semaglutide
(SET).
neutrally
charged
surfaces
jejunal
lumen
improved
preservation
proteins
efficient
traversal
barrier.
Upon
reaching
cells,
retained
positively
efficiently
overcame
apical
barrier,
intracellular
transport
basolateral
exocytosis
resulted
an
bioavailability
8.7%
notably
enhanced
pharmacological
therapeutic
effects.
This
study
successfully
addresses
some
holds
overcoming
sequential
absorption
barriers
delivery
peptides.
The
regeneration
of
bone
defects
in
diabetic
patients
still
faces
challenges,
as
the
intrinsic
healing
process
is
impaired
by
hyperglycemia.
Inspired
discovery
that
endoplasmic
reticulum
(ER)
a
state
excessive
stress
and
dysfunction
under
hyperglycemia,
leading
to
osteogenic
disorder,
novel
engineered
exosome
proposed
modulate
ER
homeostasis
for
restoring
function
mesenchymal
stem
cells
(MSCs).
results
indicate
constructed
exosomes
efficiently
regulate
dramatically
facilitate
MSCs
hyperglycemic
niche.
Additionally,
underlying
therapeutic
mechanism
elucidated.
reveal
can
directly
provide
recipient
with
SHP2
activation
mitophagy
elimination
mtROS,
which
immediate
cause
dysfunction.
To
maximize
effect
exosomes,
high-performance
hydrogel
self-healing,
bioadhesive,
exosome-conjugating
properties
applied
encapsulate
vivo
application.
In
vivo,
evaluation
defect
repair
models
demonstrates
delivering
system
intensively
enhance
osteogenesis.
These
findings
crucial
insight
into
design
biological
homeostasis-based
tissue-engineering
strategies
regeneration.
This
review
highlights
recent
progress
in
exosome-based
drug
delivery
for
cancer
therapy,
covering
exosome
biogenesis,
cargo
selection
mechanisms,
and
their
application
across
multiple
types.
As
small
extracellular
vesicles,
exosomes
exhibit
high
biocompatibility
low
immunogenicity,
making
them
ideal
vehicles
capable
of
efficiently
targeting
cells,
minimizing
off-target
damage
side
effects.
aims
to
explore
the
potential
with
a
focus
on
applications
chemotherapy,
gene
immunomodulation.
Additionally,
challenges
related
production
standardization
are
analyzed,
highlighting
importance
addressing
these
issues
clinical
application.
In
conclusion,
systems
offer
promising
future
therapies.
Further
research
should
aim
enhance
efficiency
facilitate
translation,
paving
way
innovative
treatment
strategies.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 2, 2025
Suppression
of
chimeric
antigen
receptor-modified
T
(CAR-T)
cells
by
the
immunosuppressive
tumor
microenvironment
remains
a
major
barrier
to
their
efficacy
against
solid
tumors.
To
address
this,
we
develop
an
anti-PD-L1-expressing
nanovesicle
loaded
with
STING
agonist
cGAMP
(aPD-L1
NVs@cGAMP)
remodel
and
thereby
enhance
CAR-T
cell
activity.
Following
pulmonary
delivery,
nanovesicles
rapidly
accumulate
in
lung
selectively
deliver
agonists
PD-L1-overexpressing
via
PD-1/PD-L1
interaction.
This
targeted
delivery
effectively
avoids
systemic
inflammation
poor
cellular
uptake
that
plague
free
agonists.
Internalized
trigger
signaling
induce
interferon
responses,
which
diminish
populations
such
as
myeloid-derived
suppressor
promote
infiltration.
Importantly,
anti-PD-L1
single
chain
variable
fragment
on
surface
blocks
PD-L1
upregulation
induced
prevents
exhaustion.
In
both
orthotopic
cancer
metastasis
model,
combined
therapy
aPD-L1
NVs@cGAMP
potently
inhibits
growth
recurrence.
Therefore,
is
expected
serve
effective
enhancer
improve
The
hindered
Here
authors
report
design
characterization
inhalable
cGAMP,
showing
enhanced
activity
models.
Naturally
occurring
extracellular
vesicles
(EVs)
and
synthetic
nanoparticles
like
liposomes
have
revolutionized
precision
diagnostics
medicine.
EVs
excel
in
biocompatibility
cell
targeting,
while
offer
enhanced
drug
loading
capacity
scalability.
The
clinical
translation
of
is
hindered
by
challenges
including
low
yield
heterogeneity,
whereas
face
rapid
immune
clearance
limited
targeting
efficiency.
To
bridge
these
gaps,
biomimetic
(SVs)
emerged
as
innovative
platforms,
combining
the
advantageous
properties
liposomes.
This
review
emphasizes
critical
aspects
EV
biology,
such
mechanisms
EV-cell
interaction
source-dependent
functionalities
modulation,
tissue
regeneration,
informing
SV
engineering.
We
reviewed
a
broad
array
SVs,
with
focus
on
lipid
bilayered
functionalized
proteins.
These
include
cell-derived
nanovesicles,
protein-functionalized
liposomes,
hybrid
vesicles.
By
addressing
current
highlighting
opportunities,
this
aims
to
advance
SVs
for
transformative
biomedical
applications.
The
skeleton
is
a
highly
innervated
organ
in
which
nerve
fibers
interact
with
various
skeletal
cells.
Peripheral
endings
release
neurogenic
factors
and
sense
signals,
mediate
bone
metabolism
pain.
In
recent
years,
tissue
engineering
has
increasingly
focused
on
the
effects
of
nervous
system
regeneration.
Simultaneous
regeneration
nerves
through
use
materials
or
by
enhancement
endogenous
repair
signals
been
proven
to
promote
functional
Additionally,
emerging
information
mechanisms
interoception
central
regulation
homeostasis
provide
an
opportunity
for
advancing
biomaterials.
However,
comprehensive
reviews
this
topic
are
lacking.
Therefore,
review
provides
overview
relationship
between
regeneration,
focusing
applications.
We
discuss
novel
regulatory
explore
innovative
approaches
based
nerve-bone
interactions
Finally,
challenges
future
prospects
field
briefly
discussed.
Extracellular
vesicles
(EVs),
involved
in
essential
physiological
and
pathological
processes
of
the
organism,
have
emerged
as
powerful
tools
for
disease
treatment
owing
to
their
unique
natural
biological
characteristics
artificially
acquired
advantages.
However,
limited
targeting
ability,
insufficient
production
yield,
low
drug-loading
capability
simplex
EVs
greatly
hindered
development
clinical
translation.
Therefore,
establishment
multifunctional
hybrid
membrane
nanovesicles
(HMNVs)
with
favorable
adaptability
flexibility
has
become
key
expanding
practical
application
EVs.
This
timely
review
summarizes
current
progress
HMNVs
biomedical
applications.
Different
preparation
strategies
including
physical,
chemical,
chimera
approaches
are
first
discussed.
then
individually
describes
diverse
types
based
on
homologous
or
heterologous
cell
substances,
a
fusion
liposome,
well
bacterial
membrane.
Subsequently,
specific
emphasis
is
placed
highlight
applications
toward
various
diseases
representative
examples.
Finally,
ongoing
challenges
prospects
currently
developed
translational
briefly
presented.
will
not
only
stimulate
broad
interest
among
researchers
from
disciplines
but
also
provide
valuable
insights
promising
nanoplatforms
precision
medicine.
