Apolipoprotein C3 (ApoC3) facilitates NLRP3 mediated pyroptosis of macrophages through mitochondrial damage by accelerating of the interaction between SCIMP and SYK pathway in acute lung injury

International Immunopharmacology, Год журнала: 2024, Номер 128, С. 111537 - 111537

Опубликована: Янв. 21, 2024

Язык: Английский

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The effects and mechanisms of the anti-COVID-19 traditional Chinese medicine, Dehydroandrographolide from Andrographis paniculata (Burm.f.) Wall, on acute lung injury by the inhibition of NLRP3-mediated pyroptosis

Phytomedicine, Год журнала: 2023, Номер 114, С. 154753 - 154753

Опубликована: Март 30, 2023

Dehydroandrographolide (Deh) from Andrographis paniculata (Burm.f.) Wall has strong anti-inflammatory and antioxidant activities.To explore the role of Deh in acute lung injury (ALI) coronavirus disease 19 (COVID-19) its inflammatory molecular mechanism.Liposaccharide (LPS) was injected into a C57BL/6 mouse model ALI, LPS + adenosine triphosphate (ATP) used to stimulate BMDMs an vitro ALI.In vivo considerably reduced inflammation oxidative stress by inhibiting NLRP3-mediated pyroptosis attenuated mitochondrial damage suppress through suppression ROS production Akt/Nrf2 pathway. inhibited interaction between Akt at T308 PDPK1 S549 promote protein phosphorylation. directly targeted accelerated ubiquitination. 91-GLY, 111-LYS, 126-TYR, 162-ALA, 205-ASP 223-ASP may be reason for Deh.Deh presented ALI ROS-induced inhibition pathway Therefore, it can concluded that potential therapeutic drug treatment COVID-19 or other respiratory diseases.

Язык: Английский

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Maltol, a Natural Flavor Enhancer, Inhibits NLRP3 and Non-Canonical Inflammasome Activation

Antioxidants, Год журнала: 2022, Номер 11(10), С. 1923 - 1923

Опубликована: Сен. 28, 2022

Maltol (3-hydroxy-2-methyl-4-pyrone) is used widely as a food and cosmetic supplement, it has antioxidant anti-inflammatory activities. Inflammasome causes the maturation secretion of interleukin (IL)-1β -18 through activation caspase-1 (Casp1), which contributes to various inflammatory diseases. This study examined effects maltol on inflammasome in macrophages mice. Lipopolysaccharide (LPS)-primed were treated with trigger NLRP3, NLRC4, AIM2, or non-canonical (NC) inflammasomes presence maltol. The IL-1β IL-18 cleavage Casp1 analyzed indices activation. Mice injected LPS an NLRP3 without maltol, peritoneal secretions observed. reactive oxygen species (ROS) production activity determine mechanism. inhibited NC inflammasomes, but did not alter other inflammasomes. also attenuated resulting from mechanism was revealed by inhibition ROS activity. suggested be promising anti-inflammasome molecule.

Язык: Английский

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Recent Advances in Yeast Recombinant Biosynthesis of the Triterpenoid Protopanaxadiol and Glycosylated Derivatives Thereof

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(5), С. 2197 - 2210

Опубликована: Янв. 25, 2023

Plant natural products are a seemingly endless resource for novel chemical structures. However, their extraction often results in high prices, fluctuation both quantity and quality, negative environmental impact. The latter might result from the procedure but more amount of plant biomass required. With advent synthetic biology, producing large quantities using yeasts as hosts has become possible. Here, we focus on recent advances metabolic engineering species Saccharomyces cerevisiae Yarrowia lipolytica synthesis ginsenoside triterpenoids, namely, dammarenediol-II, protopanaxadiol, protopanaxatriol, compound K, Rh1, Rh2, Rg3, F1. A discussion is provided advanced bioprocess strategies, current challenges biosynthesis triterpenoids. Finally, future directions process summarized may help reify sustainable production.

Язык: Английский

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Integrated network pharmacology, molecular docking and in vivo experiments to elucidate the extenuative mechanisms of ginseng polysaccharide against Toxoplasma gondii-induced testicular toxicity

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114147 - 114147

Опубликована: Янв. 24, 2025

Язык: Английский

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Long-Term Yeast Cultivation Coupled with In Situ Extraction for High Triterpenoid Production

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Ginsenosides are a class of triterpenoids from the ginseng genus, with many medicinal properties. Traditionally, ginsenosides extracted plants to satisfy market demand; however, this approach requires substantial plant biomass and lengthy six-year growth period before harvest. The advancement synthetic biology allows production by engineered yeast. In study, we combined our previously reported cultivation method in situ extraction enhance exportation intracellular Saccharomyces cerevisiae. Remarkably, ginsenoside reached as high 3.4 g/L single shake flask, almost 100% organic phase. "empty yeasts" were successfully reused 10 times sequential cultivations. These findings discussed context intensification for natural product synthesis. Increasing level triterpenoid synthesis facilitates rapid development supports industrialization intriguing group products.

Язык: Английский

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The potential of ginsenosides from Panax ginseng against the NLRP3 inflammasome

Phytochemistry Reviews, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Язык: Английский

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Panax ginseng as a potential therapeutic for neurological disorders associated with COVID-19; Toward targeting inflammasome

Journal of Ginseng Research, Год журнала: 2022, Номер 47(1), С. 23 - 32

Опубликована: Окт. 4, 2022

Coronavirus disease 2019 (COVID-19) is a highly infectious respiratory caused by severe acute syndrome-coronavirus-2 (SARS-CoV-2). SARS-CoV-2 infection may cause clinical manifestations of multiple organ damage, including various neurological syndromes. There are currently two oral antiviral drugs—Paxlovid and molnupiravir—that recognized to treat COVID-19, but there still no drugs that can specifically fight the challenges variants. Nucleotide-binding oligomerization domain-like receptor pyrin domain-containing-3 (NLRP3) inflammasome multimolecular complex sense heterogeneous pathogen-associated molecular patterns associated with disorders. The NLRP3 activation stimulates production caspase-1-mediated interleukin (IL)-1β, IL-18, other cytokines in immune cells. Panax (P.) ginseng medicinal plant has traditionally been widely used boost immunity pathological conditions nervous system due its safety anti-inflammatory/oxidant/viral activities. Several recent reports have indicated P. active ingredients regulate system. Therefore, this review article discusses current knowledge regarding pathogenesis disorders related COVID-19 possibility using strategy targeting pathway

Язык: Английский

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In silico and in vitro inhibition of host-based viral entry targets and cytokine storm in COVID-19 by ginsenoside compound K

Heliyon, Год журнала: 2023, Номер 9(9), С. e19341 - e19341

Опубликована: Авг. 20, 2023

SARS-CoV-2 is a novel coronavirus that emerged as an epidemic, causing respiratory disease with multiple severe symptoms and deadly consequences. ACE-2 TMPRSS2 play crucial synergistic roles in the membrane fusion viral entry of (COVID-19). The spike (S) protein binds to receptor for entry, while proteolytically cleaves S into S1 S2 subunits, promoting fusion. Therefore, are potential drug targets treating COVID-19, their inhibition promising strategy treatment prevention. This study proposes ginsenoside compound K (G-CK), triterpenoid saponin abundant Panax Ginseng, dietary medicinal herb highly consumed Korea China, effectively inhibits expression. We initially conducted in-silico evaluation where G-CK showed high affinity binding sites two target proteins SARS-CoV-2. Additionally, we evaluated stability using molecular dynamics (MD) simulations 100 ns, followed by MM-PBSA calculations. MD free energy calculations revealed has stable favorable energies, leading strong targets. Furthermore, suppressed ACE2 mRNA expression A549, Caco-2, MCF7 cells at concentration 12.5 μg/mL LPS-induced RAW 264.7 6.5 μg/mL, without significant cytotoxicity.ACE2 were significantly lower A549 following treatment. These findings suggest may evolve therapeutic against COVID-19.

Язык: Английский

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Maltol, a compound in Korean Red Ginseng, attenuates the Staphylococcus aureus–induced inflammasome activation in the skin

Journal of Ginseng Research, Год журнала: 2024, Номер 48(6), С. 609 - 615

Опубликована: Сен. 27, 2024

Язык: Английский

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