A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 452, P. 131195 - 131195

Published: March 11, 2023

Language: Английский

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Recent advancements of smartphone-based sensing technology for diagnosis, food safety analysis, and environmental monitoring

Talanta, Journal Year: 2024, Volume and Issue: 275, P. 126080 - 126080

Published: April 10, 2024

Language: Английский

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Upconversion Nanoparticles Based Sensing: From Design to Point‐of‐Care Testing

Small, Journal Year: 2024, Volume and Issue: 20(29)

Published: Feb. 28, 2024

Rare earth-doped upconversion nanoparticles (UCNPs) have achieved a wide range of applications in the sensing field due to their unique anti-Stokes luminescence property, minimized background interference, excellent biocompatibility, and stable physicochemical properties. However, UCNPs-based platforms still face several challenges, including inherent limitations from UCNPs such as low quantum yields narrow absorption cross-sections, well constraints related energy transfer efficiencies systems. Therefore, construction high-performance is an important cornerstone for conducting relevant research. This work begins by providing brief overview mechanism UCNPs. Subsequently, it offers comprehensive summary sensors' types, design principles, optimized strategies platforms. More cost-effective promising point-of-care testing implemented based on systems are also summarized. Finally, this addresses future challenges prospects

Language: Английский

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CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13577 - 13594

Published: Sept. 1, 2023

Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.

Language: Английский

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Nanomaterials-assisted CRISPR/Cas detection for food safety: Advances, challenges and future prospects

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117269 - 117269

Published: Sept. 7, 2023

Language: Английский

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Tuning the Dynamic Reaction Balance of CRISPR/Cas12a and RPA in One Pot: A Key to Switch Nucleic Acid Quantification

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(7), P. 3511 - 3519

Published: April 23, 2024

Excavating nucleic acid quantitative capabilities by combining clustered regularly interspaced short palindromic repeats (CRISPR) and isothermal amplification in one pot is of common interest. However, the mutual interference between CRISPR cleavage primary obstacle to detection. Though several works have demonstrated enhanced detection sensitivity reducing inhibition on pot, few paid attention process even dynamic reaction processes two. Herein, we find that DNA quantification can be realized regulating either recombinase polymerase (RPA) efficiency or CRISPR/Cas12a cleaving (namely, tuning balance) pot. The sensitive utilizing dual PAM-free crRNAs for recognition. varied RPA primer concentration with stabilized systems significantly affects performances. Alternatively, also achieved stabilizing while concentration. capability proved detecting targets from Lactobacillus acetotolerans SARS-CoV-2. performance toward real samples comparable real-time PCR L. spiked fermented food SARS-CoV-2 clinical samples. We expect presented method will a powerful tool quantifying other targets.

Language: Английский

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Development of multifunctional metal-organic frameworks (MOFs)-based nanofiller materials in food packaging: A comprehensive review

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 154, P. 104771 - 104771

Published: Nov. 2, 2024

Language: Английский

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CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112638 - 112638

Published: Jan. 1, 2025

Language: Английский

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CRISPR integrated biosensors: A new paradigm for cancer detection

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

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Comprehensive Approaches to Expending CRISPR Biosensor Applications Beyond Nucleic Acid Targets

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118179 - 118179

Published: Feb. 1, 2025

Language: Английский

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