International Journal of Peptide Research and Therapeutics, Год журнала: 2024, Номер 30(4)
Опубликована: Июль 2, 2024
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(1), С. 545 - 545
Опубликована: Янв. 4, 2022
Despite the great strides in healthcare during last century, some challenges still remained unanswered. The development of multi-drug resistant bacteria, alarming growth fungal infections, emerging/re-emerging viral diseases are yet a worldwide threat. Since discovery natural antimicrobial peptides able to broadly hit several pathogens, peptide-based therapeutics have been under lenses researchers. This review aims focus on synthetic and elucidate their multifaceted mechanisms action as antiviral, antibacterial antifungal agents. Antimicrobial generally affect highly preserved structures, e.g., phospholipid membrane via pore formation or other constitutive targets like peptidoglycans Gram-negative Gram-positive glucan cell wall. Additionally, particularly active biofilm destabilizing microbial communities. They can also act intracellularly, protein biosynthesis DNA replication. Their intracellular properties extended upon infection since influence steps along virus life cycle starting from receptor-cell interaction budding. Besides mode action, improvements manufacturing increase half-life performances taken into consideration together with advantages impairments clinical usage. Thus far, progress new approaches is making them promising tool counteract emerging infections.
Язык: Английский
Процитировано
72
European Journal of Microbiology and Immunology, Год журнала: 2024, Номер 14(2), С. 97 - 115
Опубликована: Апрель 22, 2024
Abstract Infectious diseases pose a formidable global challenge, compounded by the emergence of antimicrobial resistance. Consequently, researchers are actively exploring novel compounds as potential solutions. This endeavor underscores pivotal role methods employed for screening and evaluating activity—a critical step in discovery characterization agents. While traditional techniques such well-diffusion, disk-diffusion, broth-dilution commonly utilized assays, they may encounter limitations concerning reproducibility speed. Additionally, diverse array assays including cross-streaking, poisoned-food, co-culture, time-kill kinetics, resazurin assay, bioautography, etc., routinely evaluations. Advanced flow-cytometry, impedance analysis, bioluminescent technique offer rapid sensitive results, providing deeper insights into impact antimicrobials on cellular integrity. However, their higher cost limited accessibility certain laboratory settings present challenges. article provides comprehensive overview designed to characterize activity, elucidating underlying principles, protocols, advantages, limitations. The primary objective is enhance understanding methodologies agents our relentless battle against infectious diseases. By selecting appropriate testing method, can discern suitable conditions streamline identification effective
Язык: Английский
Процитировано
60
Microbiological Research, Год журнала: 2024, Номер 286, С. 127822 - 127822
Опубликована: Июнь 26, 2024
Antibiotic resistance represents a global health threat, challenging the efficacy of traditional antimicrobial agents and necessitating innovative approaches to combat infectious diseases. Among these alternatives, peptides have emerged as promising candidates against resistant pathogens. Unlike antibiotics with only one target, can use different mechanisms destroy bacteria, low toxicity mammalian cells compared many conventional antibiotics. Antimicrobial (AMPs) encouraging antibacterial properties are currently employed in clinical treatment pathogen infection, cancer, wound healing, cosmetics, or biotechnology. This review summarizes discusses drug resistance, limitations challenges AMPs peptide applications for combating drug-resistant bacterial infections, strategies enhance their capabilities.
Язык: Английский
Процитировано
51
Archives of Microbiology, Год журнала: 2024, Номер 206(2)
Опубликована: Фев. 1, 2024
Язык: Английский
Процитировано
20
Microorganisms, Год журнала: 2025, Номер 13(1), С. 156 - 156
Опубликована: Янв. 14, 2025
The intensification of aquaculture has escalated disease outbreaks and overuse antibiotics, driving the global antimicrobial resistance (AMR) crisis. Antimicrobial peptides (AMPs) provide a promising alternative due to their rapid, broad-spectrum activity, low AMR risk, additional bioactivities, including immunomodulatory, anticancer, antifouling properties. AMPs derived from aquatic invertebrates, particularly marine-derived, are well-suited for aquaculture, offering enhanced stability in high-salinity environments. This study compiles analyzes data AMP databases over 200 scientific sources, identifying approximately 350 mostly cationic α-helical, across 65 protein families. While vitro assays highlight potential, limited vivo studies hinder practical application. These could serve as feed additives, therapeutic agents, or genetic engineering approaches like CRISPR/Cas9-mediated transgenesis enhance resilience farmed species. Despite challenges such stability, ecological impacts, regulatory hurdles, advancements peptidomimetics hold significant promise. Future research should emphasize refining enhancement techniques, expanding diversity bioactivity profiles, prioritizing comprehensive evaluations. Harnessing potential represents step forward on path sustainability, reducing antibiotic dependency, combating AMR, ultimately safeguarding public health ecosystem resilience.
Язык: Английский
Процитировано
2
Advanced Biology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 6, 2025
Abstract The increasing morbidity and mortality caused by multidrug‐resistant bacteria alerts human beings to the fact that conventional antibiotics are no longer reliable effective alternatives imperatively needed. Owing wide range of sources, diverse structures, unique mode action, antimicrobial peptides have been highly anticipated extensively studied in recent years. Besides, integration artificial intelligence helps researchers gain access vast unexplored chemical space, which opens more opportunities for optimization design novel structures. Moreover, Due advances chemistry synthetic biology, also begun focus on potential mimetics peptides. In this review, a comprehensive discussion about natural synthesized as well their is made, so provide summary field inspire follow‐up research.
Язык: Английский
Процитировано
2
Life Sciences, Год журнала: 2025, Номер 366-367, С. 123485 - 123485
Опубликована: Фев. 19, 2025
Язык: Английский
Процитировано
2
Microorganisms, Год журнала: 2022, Номер 11(1), С. 24 - 24
Опубликована: Дек. 21, 2022
Enterococcus faecalis, faecium and Staphylococcus aureus are both common commensals major opportunistic human pathogens. In recent decades, these bacteria have acquired broad resistance to several classes of antibiotics, including commonly employed glycopeptides. Exemplified by vancomycin, glycopeptide is mediated through intrinsic gene mutations, and/or transferrable van cassette-carrying mobile genetic elements. Here, this review will discuss the epidemiology vancomycin-resistant S. in healthcare, community, agricultural settings, explore vancomycin context non-van development provide insights into alternative therapeutic approaches aimed at treating drug-resistant infections.
Язык: Английский
Процитировано
69
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3145 - 3145
Опубликована: Фев. 5, 2023
The skin is the largest organ of body and consists an epidermis, dermis subcutaneous adipose tissue. surface area often stated to be about 1.8 2 m2 represents our interface with environment; however, when one considers that microorganisms live in hair follicles can enter sweat ducts, interacts this aspect environment becomes 25–30 m2. Although all layers skin, including tissue, participate antimicrobial defense, review will focus mainly on role factors epidermis at surface. outermost layer stratum corneum, physically tough chemically inert which protects against numerous environmental stresses. It provides a permeability barrier attributable lipids intercellular spaces between corneocytes. In addition barrier, there innate involves lipids, peptides proteins. has low pH poor certain nutrients, limits range survive there. Melanin trans-urocanic acid provide protection from UV radiation, Langerhans cells are poised monitor local trigger immune response as needed. Each these protective barriers discussed.
Язык: Английский
Процитировано
40
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Апрель 12, 2023
Abstract Fungal infections cause more than 1.5 million deaths a year. Due to emerging antifungal drug resistance, novel strategies are urgently needed combat life-threatening fungal diseases. Here, we identify the host defense peptide mimetic, brilacidin (BRI) as synergizer with caspofungin (CAS) against CAS-sensitive and CAS-resistant isolates of Aspergillus fumigatus, Candida albicans , C. auris CAS-intrinsically resistant Cryptococcus neoformans . BRI also potentiates azoles A. fumigatus several Mucorales fungi. acts in by affecting cell wall integrity pathway membrane potential. combined CAS significantly clears lung infection an immunosuppressed murine model invasive pulmonary aspergillosis. alone decreases burden ablates disease development keratitis. Our results indicate that combinations drugs clinical use likely improve treatment outcome aspergillosis other infections.
Язык: Английский
Процитировано
37