Budding yeast as an ideal model for elucidating the role of N6‐methyladenosine in regulating gene expression DOI Creative Commons
Waleed S. Albihlal, Wei Yee Chan, Folkert J. van Werven

et al.

Yeast, Journal Year: 2024, Volume and Issue: 41(4), P. 148 - 157

Published: Jan. 18, 2024

Abstract N 6 ‐methyladenosine (m6A) is a highly abundant and evolutionarily conserved messenger RNA (mRNA) modification. This modification installed on RRACH motifs mRNAs by hetero‐multimeric holoenzyme known as m6A methyltransferase complex (MTC). The mark then recognised group of proteins the YTH domain family which guide mRNA for subsequent downstream processes that determine its fate. In yeast, thousands during early meiosis MTC m6A‐modified are read domain‐containing protein Mrb1/Pho92. this review, we aim to delve into recent advances in our understanding regulation roles yeast meiosis. We will discuss potential functions translation decay, unravelling their significance regulating gene expression. propose serves an exceptional model organism study fundamental molecular mechanisms related function mRNAs. insights gained from research not only expand knowledge modifications but also offer valuable broader landscape eukaryotic posttranscriptional

Language: Английский

FIONA1‐mediated mRNA m6A methylation regulates the response of Arabidopsis to salt stress DOI
Jing Cai, Jianzhong Hu, Tao Xu

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(3), P. 900 - 912

Published: Jan. 9, 2024

Abstract N 6 ‐methyladenosine (m A) is an mRNA modification widely found in eukaryotes and plays a crucial role plant development stress responses. FIONA1 (FIO1) recently identified m A methyltransferase that regulates Arabidopsis ( thaliana ) floral transition; however, its response remains unknown. In this study, we demonstrate FIO1‐mediated methylation vital salt Arabidopsis. The loss‐of‐function fio1 mutant was sensitive to stress. Importantly, the complementation lines expressing wild‐type FIO1 exhibited phenotype, whereas , which two critical amino acid residues essential for activity were mutated, did not recover phenotype under stress, indicating sensitivity associated with activity. Furthermore, regulated ROS production affected transcript level of several stress‐responsive genes via modulating their stability A‐dependent manner by specifically targeting differentially compared other writer. Collectively, our findings highlight molecular mechanism adaptation.

Language: Английский

Citations

15
Crosstalk between RNA m6A modification and epigenetic factors in plant gene regulation DOI Creative Commons
Jianzhong Hu, Tao Xu, Hunseung Kang

et al.

Plant Communications, Journal Year: 2024, Volume and Issue: 5(10), P. 101037 - 101037

Published: July 6, 2024

N6-methyladenosine (m6A) is the most abundant modification observed in eukaryotic mRNAs. Advances transcriptome-wide m6A mapping and sequencing technologies have enabled identification of several conserved motifs plants, including RRACH (R = A/G H A/C/U) UGUAW (W U or A) motifs. However, mechanisms underlying deposition marks at specific positions individual transcripts remain to be clarified. Evidence from plant animal studies suggests that writer eraser components are recruited genomic loci through interactions with particular transcription factors, 5-methylcytosine DNA methylation marks, histone marks. In addition, recent cells shown microRNAs play a role depositing sites base-pairing mechanism. also affects biogenesis function chromatin-associated regulatory RNAs long noncoding RNAs. Although we less an understanding link between epigenetic factors plants than animals, progress identifying proteins interact has provided insights into crosstalk which plays crucial transcript-specific regulation plants.

Language: Английский

Citations

12
COOLAIR and PRC2 function in parallel to silence FLC during vernalization DOI Creative Commons
Mathias Nielsen, Govind Menon, Yusheng Zhao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(4)

Published: Jan. 18, 2024

Noncoding transcription induces chromatin changes that can mediate environmental responsiveness, but the causes and consequences of these mechanisms are still unclear. Here, we investigate how antisense (termed COOLAIR ) interfaces with Polycomb Repressive Complex 2 (PRC2) silencing during winter-induced epigenetic regulation Arabidopsis FLOWERING LOCUS C ( FLC ). We use genetic analyses on lines ineffective or hyperactive for pathway in combination computational modeling to define underlying repression. Our results show is silenced through pathways function different dynamics: a transcription-mediated capable fast response parallel slow PRC2 switching mechanism maintains each allele an epigenetically state. Components both regulated by common transcriptional regulator (NTL8), which accumulates reduced dilution due growth at low temperature. The activities regulatory steps, their control temperature-dependent dynamics, create flexible system registering widely fluctuating natural temperature conditions change year year, yet ensure robust FLC.

Language: Английский

Citations

10
Epigenetics in the modern era of crop improvements DOI Creative Commons
Yan Xue, Xiaofeng Cao, Xiangsong Chen

et al.

Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body knowledge on both locus-specific genome-wide patterns. Studies initially grounded in model Arabidopsis have been broadened encompass diverse array crop species, revealing multifaceted roles epigenetics physiological agronomic traits. With recent technological advancements, epigenetic regulations at single-cell level large-scale population emerging as new focuses. This review offers an in-depth synthesis regulations, detailing catalytic machinery functions. It delves into intricate interplay among various elements their collective influence modulation Furthermore, it examines breakthroughs technologies for modifications integration strategies improvement. The underscores transformative potential bolstering performance, advocating development efficient tools fully exploit agricultural benefits insights.

Language: Английский

Citations

1
NERD-dependent m6A modification of the nascent FLC transcript regulates flowering time in Arabidopsis DOI
Yanlin Shao, Jinqi Ma, Songyao Zhang

et al.

Nature Plants, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

Citations

1
Principles, functions, and biological implications of m6A in plants DOI Open Access
Peizhe Song, Zhihe Cai,

Guifang Jia

et al.

RNA, Journal Year: 2024, Volume and Issue: 30(5), P. 491 - 499

Published: March 26, 2024

Over the past decade, N 6 -methyladenosine (m A) has emerged as a prevalent and dynamically regulated modification across transcriptome; it been reversibly installed, removed, interpreted by specific binding proteins, played crucial roles in molecular biological processes. Within this scope, we consolidate recent advancements of m A research plants regarding gene expression regulation, diverse physiologic pathogenic processes, well crop trial implications, to guide discussions on challenges associated with leveraging epitranscriptome editing for improvement.

Language: Английский

Citations

7
ECT12, an YTH-domain protein, is a potential mRNA m6A reader that affects abiotic stress responses by modulating mRNA stability in Arabidopsis DOI Open Access

Umme Amara,

Jianzhong Hu, Su Jung Park

et al.

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 206, P. 108255 - 108255

Published: Dec. 3, 2023

Language: Английский

Citations

14
TMK4-mediated FIP37 phosphorylation regulates auxin-triggered N-methyladenosine modification of auxin biosynthetic genes in Arabidopsis DOI Creative Commons
Bin Li,

Qiting Zhou,

Linjun Cai

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(8), P. 114597 - 114597

Published: Aug. 1, 2024

The dynamics of N

Language: Английский

Citations

5
Reading m6A marks in mRNA: A potent mechanism of gene regulation in plants DOI Creative Commons

Thi Kim Hang Nguyen,

Hunseung Kang

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(12), P. 2586 - 2599

Published: Oct. 4, 2024

ABSTRACT Modifications to RNA have recently been recognized as a pivotal regulator of gene expression in living organisms. More than 170 chemical modifications identified RNAs, with N 6 ‐methyladenosine (m A) being the most abundant modification eukaryotic mRNAs. The addition and removal m A marks are catalyzed by methyltransferases (referred “writers”) demethylases “erasers”), respectively. In addition, mRNAs interpreted A‐binding proteins “readers”), which regulate fate mRNAs, including stability, splicing, transport, translation. Therefore, exploring mechanism underlying reader‐mediated modulation metabolism is essential for much deeper understanding epigenetic role plants. Recent discoveries improved our functions readers plant growth development, stress response, disease resistance. This review highlights latest developments reader research, emphasizing diverse RNA‐binding domains crucial function biological cellular roles response developmental environmental signals. Moreover, we propose discuss potential future research directions challenges identifying novel elucidating mechanistic

Language: Английский

Citations

5
ECT8, an mRNA m6A reader, enhances salt stress tolerance by modulating mRNA stability in Arabidopsis DOI

Thi Kim Hang Nguyen,

Umme Amara,

Hunseung Kang

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Abstract N6‐methyladenosine (m 6 A), the most prevalent modification found in eukaryotic mRNAs, is recognized and interpreted by m A‐binding proteins called A readers. The EVOLUTIONARILY CONSERVED C‐TERMINAL REGION (ECT) have increasingly been identified as readers plants. recent study has demonstrated that loss‐of‐function ect8 mutant sensitive to salt stress enhancing stability of negative regulators Arabidopsis ( thaliana ). In this study, we generated analyzed ECT8‐overexpressing transgenic plants further explore function ECT8 response. electrophoretic mobility shift assay vitro showed binds A‐modified synthetic RNAs, preferring UGUm AA UACm AGA motifs over GGm ACU motif. Contrary exhibiting hypersensitivity regulators, displayed tolerance increasing expression levels positive regulators. Moreover, RNA‐immunoprecipitation stress‐responsive mRNAs planta . Collectively, our current previous findings highlight ECT8‐mediated stabilization destabilization genes encoding or respectively, contribute Arabidopsis.

Language: Английский

Citations

0