Multimodal Noninvasive Functional Neurophotonic Imaging of Murine Brain‐Wide Sensory Responses

Advanced Science, Год журнала: 2022, Номер 9(24)

Опубликована: Июль 7, 2022

Modern optical neuroimaging approaches are expanding the ability to elucidate complex brain function. Diverse imaging contrasts enable direct observation of neural activity with functional sensors along induced hemodynamic responses. To date, decoupling interplay neurovascular coupling and dynamical physiological states has remained challenging when employing single-modality readings. A hybrid fluorescence optoacoustic tomography platform combined a custom data processing pipeline based on statistical parametric mapping is devised, attaining first noninvasive simultaneous calcium activation patterns using contrasts. Correlated changes in oxy- deoxygenated hemoglobin, total oxygen saturation, rapid GCaMP6f signals observed response peripheral sensory stimulation. While concurrent epifluorescence serves corroborate complement observations, latter further aids responses from slowly varying background recordings mediated by changes. The expands capabilities conventional methods provide more comprehensive readings for studying neurometabolic mechanisms related diseases.

Язык: Английский

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A practical guide for model-based reconstruction in optoacoustic imaging

Frontiers in Physics, Год журнала: 2022, Номер 10

Опубликована: Ноя. 1, 2022

Optoacoustic (OA, photoacoustic) imaging capitalizes on the low scattering of ultrasound within biological tissues to provide optical absorption-based contrast with high resolution at depths not reachable microscopy. For deep tissue applications, OA image formation commonly relies acoustic inversion time-resolved tomographic data. The excitation responses and subsequent propagation waves can be mathematically described as a forward model enabling reconstruction via algebraic inversion. These model-based methods have been shown outperform alternative approaches further render images from incomplete datasets, strongly distorted signals or other suboptimally recorded Herein, we general perspective methods, review recent progress, discuss performance different algorithms under practical scenarios.

Язык: Английский

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Multiparametric Brain Hemodynamics Imaging Using a Combined Ultrafast Ultrasound and Photoacoustic System

Advanced Science, Год журнала: 2024, Номер 11(31)

Опубликована: Июнь 17, 2024

Abstract Studying brain‐wide hemodynamic responses to different stimuli at high spatiotemporal resolutions can help gain new insights into the mechanisms of neuro‐ diseases and ‐disorders. Nonetheless, this task is challenging, primarily due complexity neurovascular coupling, which encompasses interdependent parameters including cerebral blood volume (CBV), flow (CBF), oxygen saturation (SO 2 ). The current brain imaging technologies exhibit inherent limitations in resolution, sensitivity, depth, restricting their capacity comprehensively capture intricacies functions. To address this, a multimodal functional ultrasound photoacoustic (fUSPA) platform reported, integrates ultrafast multispectral methods compact head‐mountable device, quantitatively map individual dynamics CBV, CBF, SO as well contrast agent enhanced resolutions. Following systematic characterization, fUSPA system applied study cerebrovascular reactivity (CVR) single‐vessel resolution via relative changes response hypercapnia stimulation. These results show that cortical veins arteries differences CVR stimulated state consistent anti‐correlation CBV oscillations during resting state, demonstrating multiparametric system's unique capabilities investigating complex

Язык: Английский

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Early screening model for mild cognitive impairment based on resting-state functional connectivity: a functional near-infrared spectroscopy study

Neurophotonics, Год журнала: 2022, Номер 9(04)

Опубликована: Дек. 6, 2022

SignificanceAs an early stage of Alzheimer's disease (AD), the diagnosis amnestic mild cognitive impairment (aMCI) has important clinical value for timely intervention AD. Functional near-infrared spectroscopy (fNIRS)-based resting-state brain connectivity analysis, which could provide economic and quick screening strategy aMCI, remains to be extensively investigated.AimThis study aimed verify feasibility fNIRS-based evaluating function in patients with determine model auxiliary diagnosis.ApproachThe fNIRS was utilized exploring changes functional 64 aMCI. The region interest (ROI)-based channel-based connections significant inter-group differences have been extracted through two-sample t-tests receiver operating characteristic (ROC). These specificity sensitivity were then taken as features classification.ResultsCompared healthy controls, MCI group significantly reduced between bilateral prefrontal, parietal, occipital, right temporal lobes. Specifically, long-range from prefrontal occipital lobe, parietal exhibited stronger identifiability (area under ROC curve > 0.65, **p < 0.01). Subsequently, optimal classification accuracy ROI-based 71.59%. Furthermore, most responsive located dorsolateral lobe left concomitant highest 73.86%.ConclusionOur findings indicate that analysis support diagnosis. Notably, involving lobes potential efficient biomarkers.

Язык: Английский

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Hybrid magnetic resonance and optoacoustic tomography (MROT) for preclinical neuroimaging

Light Science & Applications, Год журнала: 2022, Номер 11(1)

Опубликована: Ноя. 24, 2022

Abstract Multi-modal imaging is essential for advancing our understanding of brain function and unraveling pathophysiological processes underlying neurological psychiatric disorders. Magnetic resonance (MR) optoacoustic (OA) have been shown to provide highly complementary contrasts capabilities preclinical neuroimaging. True integration between these modalities can thus offer unprecedented studying the rodent in action. We report on a hybrid magnetic tomography (MROT) system concurrent noninvasive structural functional mouse brain. Volumetric OA was designed as an insert into high-field MR scanner by integrating customized MR-compatible spherical transducer array, illumination module, dedicated radiofrequency coil. A tailored data processing pipeline has developed mitigate signal crosstalk accurately register image volumes acquired with T1-weighted, angiography, blood oxygenation level-dependent (BOLD) sequences onto corresponding vascular recorded modality. demonstrate acquisition dual-mode anatomical angiographic images scanner, well real-time readings multiple hemodynamic parameters from animals subjected stress. Our approach combines molecular advantages superb soft-tissue contrast MR, further providing excellent platform cross-validation two modalities.

Язык: Английский

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Full-view LED-based optoacoustic tomography

Photoacoustics, Год журнала: 2023, Номер 31, С. 100521 - 100521

Опубликована: Июнь 1, 2023

Optoacoustic tomography is commonly performed with bulky and expensive short-pulsed solid-state lasers providing high per-pulse energies in the millijoule range. Light emitting diodes (LEDs) represent a cost-effective portable alternative for optoacoustic signal excitation that can additionally provide excellent pulse-to-pulse stability. Herein, we introduce full-view LED-based (FLOAT) system deep tissue vivo imaging. It based on custom-made electronic unit driving stacked array of LEDs, which attains 100 ns pulse width highly stable (0.62 % standard deviation) total energy 0.48 mJ. The illumination source integrated into circular cylindrically-focused ultrasound detection elements to result tomographic configuration, plays critical role circumventing limited-view effects, enhancing effective field-of-view image quality cross-sectional (2D) We characterized FLOAT performance terms width, power stability, light distribution, signal-to-noise penetration depth. human finger revealed comparable imaging achieved pulsed Nd:YAG laser. anticipated this compact, affordable versatile technology will facilitate developments resource-limited settings biological clinical applications.

Язык: Английский

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Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice

Advanced Science, Год журнала: 2022, Номер 10(3)

Опубликована: Ноя. 27, 2022

Abstract Functional magnetic resonance imaging (fMRI) has massively contributed to the understanding of mammalian brain function. However, origin and interpretation blood oxygen level‐dependent (BOLD) signals retrieved by fMRI remain highly disputed. This article reports on development a fully hybridized system enabling concurrent functional optoacoustic tomography (MROT) measurements stimulus‐evoked brain‐wide sensory responses in mice. The complementary angiographic soft tissue contrasts both modalities along with simultaneous multi‐parametric readings hemodynamic are leveraged order establish unequivocal links between various counteracting physiological metabolic processes brain. results indicate that BOLD correlated, spatially temporally, total hemoglobin resolved volumetric multi‐spectral tomography. Furthermore, differential oxygenated deoxygenated exhibit superior sensitivity as compared when detecting responses. MROT approach greatly expands neuroimaging toolset comprehensively study neurovascular neurometabolic coupling mechanisms related diseases.

Язык: Английский

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Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 25, 2024

Abstract Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding function. The existing neuroimaging techniques are largely limited by restricted field view, slow speed, or otherwise do not have the adequate spatial to capture activities on a capillary and cellular level. To address these limitations, we introduce fluorescence localization microscopy aided sparsely-labeled red blood cells cortex-wide morphological functional cerebral angiography 4.9 µm 1 s temporal resolution. When combined calcium imaging, proposed method enables extended recordings stimulus-evoked neuro-vascular changes in murine while providing simultaneous multiparametric readings intracellular neuronal activity, flow velocity/direction/volume, vessel diameter. Owing its simplicity versatility, approach will become an invaluable tool deciphering regulation cortical microcirculation neurovascular coupling health disease.

Язык: Английский

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Prediction of health anxiety using resting-state functional near-infrared spectroscopy and machine learning

Journal of Affective Disorders, Год журнала: 2025, Номер 374, С. 39 - 45

Опубликована: Янв. 8, 2025

Язык: Английский

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Multimodal imaging of murine cerebrovascular dynamics induced by transcranial pulse stimulation

Alzheimer s & Dementia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Transcranial pulse stimulation (TPS) is increasingly being investigated as a promising potential treatment for Alzheimer's disease (AD). Although the safety and preliminary clinical efficacy of TPS short pulses have been supported by neuropsychological scores in treated AD patients, its fundamental mechanisms are uncharted. Herein, we used multi-modal preclinical imaging platform combining real-time volumetric optoacoustic tomography, contrast-enhanced magnetic resonance imaging, ex vivo immunofluorescence to comprehensively analyze structural hemodynamic effects induced TPS. Cohorts healthy transgenic mice were imaged during after exposure at various per-pulse energy levels. enhanced microvascular network, whereas blood-brain barrier remained intact following procedure. Notably, higher energies necessary induce changes mice, arguably due their impacted vessels. These findings shed light on cerebrovascular dynamics treatment, hence expected assist improving therapeutic outcomes. ·Transcranial facilitates transcranial wave propagation using avoid tissue heating. ·Preclinical combines (OA) (CE-MRI), ·Blood volume enhancement networks was reproducibly observed with OA stimulation. ·CE-MRI gross pathology further confirmed that brain architecture maintained without (BBB) opening exposure, thus validating ·Higher compared wild-type animals, pathological

Язык: Английский

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