Retinotectal circuitry of larval zebrafish is adapted to detection and pursuit of prey

eLife, Год журнала: 2020, Номер 9

Опубликована: Окт. 12, 2020

Retinal axon projections form a map of the visual environment in tectum. A zebrafish larva typically detects prey object its peripheral field. As it turns and swims towards prey, stimulus enters central, binocular area, seemingly expands size. By volumetric calcium imaging, we show that posterior tectal neurons, which serve to detect at distance, tend respond small objects intrinsically compute their direction movement. Neurons anterior tectum, where image is represented shortly before capture strike, are tuned larger sizes frequently not direction-selective, indicating mainly interocular comparisons an object’s movement close range. The feature originates from linear combination diverse, functionally specialized, lamina-specific, topographically ordered retinal ganglion cell synaptic inputs. We conclude local cell-type composition connectivity across tectum adapted processing location-dependent, behaviorally relevant features.

Язык: Английский

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Partitioning of gene expression among zebrafish photoreceptor subtypes

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Авг. 30, 2021

Abstract Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- bright-light vision, respectively. While many molecular features that distinguish cones known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes renowned the diversity of their photoreceptor systems. Here, we used single-cell RNA-seq to profile adult in zebrafish, a teleost. We found addition four canonical zebrafish types, there exist subpopulations green red (previously shown be located ventral retina) express red-shifted opsin paralogs ( opn1mw4 or opn1lw1 ) as well unique combination phototransduction genes. Furthermore, paralogous genes is partitioned subtypes, analogous partitioning between seen across vertebrates. The cone-gene pairs arose via teleost-specific whole-genome duplication later clade-specific duplications. also discovered distinct transcriptional regulators, including factors not previously implicated development differentiation. Overall, our work suggests action differentially expressed regulators enables diversification teleosts.

Язык: Английский

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Ancestral circuits for vertebrate color vision emerge at the first retinal synapse

Science Advances, Год журнала: 2021, Номер 7(42)

Опубликована: Окт. 13, 2021

In vivo recordings of cone photoreceptor outputs in a tetrachromate reveal efficient ancestral strategy for color processing.

Язык: Английский

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Anatomy and function of retinorecipient arborization fields in zebrafish

The Journal of Comparative Neurology, Год журнала: 2021, Номер 529(15), С. 3454 - 3476

Опубликована: Июнь 28, 2021

Abstract In 1994, Burrill and Easter described the retinal projections in embryonic larval zebrafish, introducing term “arborization fields” (AFs) for retinorecipient areas. AFs were numbered from 1 to 10 according their positions along optic tract. With exception of AF10 (neuropil tectum), annotations remained tentative. Here we offer an update on likely identities functions zebrafish after successfully matching classical neuroanatomy digital Max Planck Zebrafish Brain Atlas. our system, individual are neuropil areas associated with following nuclei: AF1 suprachiasmatic nucleus; AF2 posterior parvocellular preoptic AF3 AF4 ventrolateral thalamic anterior intermediate nuclei; AF5 dorsal accessory AF7 superficial pretectal AF8 central AF9d AF9v ventral periventricular nuclei. AF6 is probably part system. Imaging, ablation, activation experiments showed contributions potentially optokinetic optomotor reflexes, phototaxis, prey detection. AF6, respond dimming, brightening. While few remain tentative, it apparent that visual system anatomically functionally continuous its adult successor fits general cyprinid pattern. This study illustrates synergy created by merging a cellular‐resolution brain atlas resource functional imaging zebrafish.

Язык: Английский

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Birds multiplex spectral and temporal visual information via retinal On- and Off-channels

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Авг. 31, 2023

In vertebrate vision, early retinal circuits divide incoming visual information into functionally opposite elementary signals: On and Off, transient sustained, chromatic achromatic. Together these signals can yield an efficient representation of the scene for transmission to brain via optic nerve. However, this long-standing interpretation function is based on mammals, it unclear whether functional arrangement common all vertebrates. Here we show that male poultry chicks use a fundamentally different strategy communicate from eye brain. Rather than using pairs output channels, encode polarity, timing, spectral composition stimuli in highly correlated manner: fast achromatic encoded by Off-circuits, slow overwhelmingly On-circuits. Moreover, most channels combine On- Off-circuits simultaneously encode, or multiplex, both information. Our results birds conform evidence fish, amphibians, reptiles which retain full ancestral complement four types cone photoreceptors.

Язык: Английский

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From perception to behavior: The neural circuits underlying prey hunting in larval zebrafish

Frontiers in Neural Circuits, Год журнала: 2023, Номер 17

Опубликована: Фев. 1, 2023

A key challenge for neural systems is to extract relevant information from the environment and make appropriate behavioral responses. The larval zebrafish offers an exciting opportunity studying these sensing processes sensory-motor transformations. Prey hunting instinctual behavior of that requires brain combine different attributes sensory input form motor outputs. Due its small size transparency allows optical recording whole-brain activity reveal mechanisms involved in prey capture. In this review we discuss how visual identify locate prey, circuits governing generation commands response can be modulated by internal states experience, some outstanding questions field.

Язык: Английский

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Different spectral sensitivities of ON- and OFF-motion pathways enhance the detection of approaching color objects in Drosophila

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 24, 2023

Color and motion are used by many species to identify salient objects. They processed largely independently, but color contributes processing in humans, for example, enabling moving colored objects be detected when their luminance matches the background. Here, we demonstrate an unexpected, additional contribution of vision Drosophila. We show that behavioral ON-motion responses more sensitive UV than OFF-motion, cellular pathways connecting UV-sensitive R7 photoreceptors ON OFF-motion-sensitive T4 T5 cells, using neurogenetics calcium imaging. Remarkably, this circuitry enhances detection approaching discs, not green discs with same chromatic contrast, how could generalize systems ON- OFF-motion pathways. Our results provide a computational circuit basis favor saliently

Язык: Английский

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Ultraviolet vision in anemonefish improves colour discrimination

Journal of Experimental Biology, Год журнала: 2024, Номер 227(7)

Опубликована: Апрель 1, 2024

ABSTRACT In many animals, ultraviolet (UV) vision guides navigation, foraging, and communication, but few studies have addressed the contribution of UV signals to colour vision, or measured discrimination thresholds using behavioural experiments. Here, we tested in an anemonefish (Amphiprion ocellaris) a five-channel (RGB-V-UV) LED display. We first determined that maximal sensitivity A. ocellaris cone was ∼386 nm microspectrophotometry. Three additional spectral sensitivities had maxima at ∼497, 515 ∼535 nm. then behaviourally by training distinguish coloured target pixel from grey distractor pixels varying intensity. Thresholds were calculated for nine sets colours with without signals. Using tetrachromatic model, found better (i.e. lower) discriminating when higher chromatic contrast. These caused greater stimulation relative other types. findings imply component cues improves their detectability, which likely increases prominence body patterns communication silhouette zooplankton prey.

Язык: Английский

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Circuit mechanisms for colour vision in zebrafish

Current Biology, Год журнала: 2021, Номер 31(12), С. R807 - R820

Опубликована: Июнь 1, 2021

The use of spectral information in natural light to inform behaviour is one the oldest and most fundamental abilities visual systems. It long-predates animals' venture onto land, even appearance image-forming eyes. Accordingly, circuits for colour vision evolved under surface ancient oceans hundreds millions years. These aquatic beginnings fundamentally underpin, likely constrain, organisation modern In contrast our detailed circuit level understanding from diverse terrestrial vertebrates, however, comparatively little known about their counterparts. Here, I summarise some what neural fish, species-diverse group vertebrates. With a focus on zebrafish, will explore how computational strategies are linked statistics underwater world, study might help us understand general, including own

Язык: Английский

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Biochemistry and physiology of zebrafish photoreceptors

Pflügers Archiv - European Journal of Physiology, Год журнала: 2021, Номер 473(9), С. 1569 - 1585

Опубликована: Фев. 17, 2021

Abstract All vertebrates share a canonical retina with light-sensitive photoreceptors in the outer retina. These are of two kinds: rods and cones, adapted to low bright light conditions, respectively. They both show peculiar morphology, long segments, comprised ordered stacks disc-shaped membranes. discs host numerous proteins, many which contribute visual transduction cascade. This pathway converts stimulus into biological signal, ultimately modulating synaptic transmission. Recently, zebrafish ( Danio rerio ) has gained popularity for studying function vertebrate photoreceptors. In this review, we introduce model system its contribution our understanding photoreception focus on cone

Язык: Английский

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