Partitioning of gene expression among zebrafish photoreceptor subtypes

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Aug. 30, 2021

Abstract Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- bright-light vision, respectively. While many molecular features that distinguish cones known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes renowned the diversity of their photoreceptor systems. Here, we used single-cell RNA-seq to profile adult in zebrafish, a teleost. We found addition four canonical zebrafish types, there exist subpopulations green red (previously shown be located ventral retina) express red-shifted opsin paralogs ( opn1mw4 or opn1lw1 ) as well unique combination phototransduction genes. Furthermore, paralogous genes is partitioned subtypes, analogous partitioning between seen across vertebrates. The cone-gene pairs arose via teleost-specific whole-genome duplication later clade-specific duplications. also discovered distinct transcriptional regulators, including factors not previously implicated development differentiation. Overall, our work suggests action differentially expressed regulators enables diversification teleosts.

Language: Английский

---

Ancestral circuits for vertebrate color vision emerge at the first retinal synapse

Science Advances, Journal Year: 2021, Volume and Issue: 7(42)

Published: Oct. 13, 2021

In vivo recordings of cone photoreceptor outputs in a tetrachromate reveal efficient ancestral strategy for color processing.

Language: Английский

---

Birds multiplex spectral and temporal visual information via retinal On- and Off-channels

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 31, 2023

In vertebrate vision, early retinal circuits divide incoming visual information into functionally opposite elementary signals: On and Off, transient sustained, chromatic achromatic. Together these signals can yield an efficient representation of the scene for transmission to brain via optic nerve. However, this long-standing interpretation function is based on mammals, it unclear whether functional arrangement common all vertebrates. Here we show that male poultry chicks use a fundamentally different strategy communicate from eye brain. Rather than using pairs output channels, encode polarity, timing, spectral composition stimuli in highly correlated manner: fast achromatic encoded by Off-circuits, slow overwhelmingly On-circuits. Moreover, most channels combine On- Off-circuits simultaneously encode, or multiplex, both information. Our results birds conform evidence fish, amphibians, reptiles which retain full ancestral complement four types cone photoreceptors.

Language: Английский

---

From perception to behavior: The neural circuits underlying prey hunting in larval zebrafish

Frontiers in Neural Circuits, Journal Year: 2023, Volume and Issue: 17

Published: Feb. 1, 2023

A key challenge for neural systems is to extract relevant information from the environment and make appropriate behavioral responses. The larval zebrafish offers an exciting opportunity studying these sensing processes sensory-motor transformations. Prey hunting instinctual behavior of that requires brain combine different attributes sensory input form motor outputs. Due its small size transparency allows optical recording whole-brain activity reveal mechanisms involved in prey capture. In this review we discuss how visual identify locate prey, circuits governing generation commands response can be modulated by internal states experience, some outstanding questions field.

Language: Английский

---

Different spectral sensitivities of ON- and OFF-motion pathways enhance the detection of approaching color objects in Drosophila

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 24, 2023

Color and motion are used by many species to identify salient objects. They processed largely independently, but color contributes processing in humans, for example, enabling moving colored objects be detected when their luminance matches the background. Here, we demonstrate an unexpected, additional contribution of vision Drosophila. We show that behavioral ON-motion responses more sensitive UV than OFF-motion, cellular pathways connecting UV-sensitive R7 photoreceptors ON OFF-motion-sensitive T4 T5 cells, using neurogenetics calcium imaging. Remarkably, this circuitry enhances detection approaching discs, not green discs with same chromatic contrast, how could generalize systems ON- OFF-motion pathways. Our results provide a computational circuit basis favor saliently

Language: Английский

---

Anatomy and function of retinorecipient arborization fields in zebrafish

The Journal of Comparative Neurology, Journal Year: 2021, Volume and Issue: 529(15), P. 3454 - 3476

Published: June 28, 2021

Abstract In 1994, Burrill and Easter described the retinal projections in embryonic larval zebrafish, introducing term “arborization fields” (AFs) for retinorecipient areas. AFs were numbered from 1 to 10 according their positions along optic tract. With exception of AF10 (neuropil tectum), annotations remained tentative. Here we offer an update on likely identities functions zebrafish after successfully matching classical neuroanatomy digital Max Planck Zebrafish Brain Atlas. our system, individual are neuropil areas associated with following nuclei: AF1 suprachiasmatic nucleus; AF2 posterior parvocellular preoptic AF3 AF4 ventrolateral thalamic anterior intermediate nuclei; AF5 dorsal accessory AF7 superficial pretectal AF8 central AF9d AF9v ventral periventricular nuclei. AF6 is probably part system. Imaging, ablation, activation experiments showed contributions potentially optokinetic optomotor reflexes, phototaxis, prey detection. AF6, respond dimming, brightening. While few remain tentative, it apparent that visual system anatomically functionally continuous its adult successor fits general cyprinid pattern. This study illustrates synergy created by merging a cellular‐resolution brain atlas resource functional imaging zebrafish.

Language: Английский

---

Circuit mechanisms for colour vision in zebrafish

Current Biology, Journal Year: 2021, Volume and Issue: 31(12), P. R807 - R820

Published: June 1, 2021

The use of spectral information in natural light to inform behaviour is one the oldest and most fundamental abilities visual systems. It long-predates animals' venture onto land, even appearance image-forming eyes. Accordingly, circuits for colour vision evolved under surface ancient oceans hundreds millions years. These aquatic beginnings fundamentally underpin, likely constrain, organisation modern In contrast our detailed circuit level understanding from diverse terrestrial vertebrates, however, comparatively little known about their counterparts. Here, I summarise some what neural fish, species-diverse group vertebrates. With a focus on zebrafish, will explore how computational strategies are linked statistics underwater world, study might help us understand general, including own

Language: Английский

---

Biochemistry and physiology of zebrafish photoreceptors

Pflügers Archiv - European Journal of Physiology, Journal Year: 2021, Volume and Issue: 473(9), P. 1569 - 1585

Published: Feb. 17, 2021

Abstract All vertebrates share a canonical retina with light-sensitive photoreceptors in the outer retina. These are of two kinds: rods and cones, adapted to low bright light conditions, respectively. They both show peculiar morphology, long segments, comprised ordered stacks disc-shaped membranes. discs host numerous proteins, many which contribute visual transduction cascade. This pathway converts stimulus into biological signal, ultimately modulating synaptic transmission. Recently, zebrafish ( Danio rerio ) has gained popularity for studying function vertebrate photoreceptors. In this review, we introduce model system its contribution our understanding photoreception focus on cone

Language: Английский

---

Zebrafish Models of Photoreceptor Dysfunction and Degeneration

Biomolecules, Journal Year: 2021, Volume and Issue: 11(1), P. 78 - 78

Published: Jan. 9, 2021

Zebrafish are an instrumental system for the generation of photoreceptor degeneration models, which can be utilized to determine underlying causes dysfunction and death, analysis potential therapeutic compounds, as well characterization regenerative responses. We review wealth information from existing zebrafish models disease, specifically they relate currently accepted taxonomic classes human rod cone disease. also highlight that rich, detailed derived studying development, structure, function, including behavioural assessments in vivo imaging zebrafish. available a diversity diseases, dystrophies, challenging recapitulate nocturnal mammalian systems. Newly discovered disease drusenoid deposit formation may not only provide important insights into pathogenesis but approaches. have already shown their use providing pre-clinical data prior testing genetic therapies clinical trials, such antisense oligonucleotide therapy Usher syndrome.

Language: Английский

---

Dendrobium nobile protects retinal cells from UV-induced oxidative stress damage via Nrf2/HO-1 and MAPK pathways

Journal of Ethnopharmacology, Journal Year: 2021, Volume and Issue: 288, P. 114886 - 114886

Published: Nov. 30, 2021

Language: Английский

---