Biosensor-based methods for exosome detection with applications to disease diagnosis

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117362 - 117362

Опубликована: Март 1, 2025

Язык: Английский

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Recent advances of surface plasmon resonance sensors based on metal-organic frameworks

Talanta, Год журнала: 2025, Номер unknown, С. 128008 - 128008

Опубликована: Март 1, 2025

Язык: Английский

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

ACS Sensors, Год журнала: 2023, Номер 8(11), С. 3988 - 4007

Опубликована: Окт. 23, 2023

Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development

Язык: Английский

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DNA Gate-Based CRISPR-Cas Exponential Amplification System for Ultrasensitive Small Extracellular Vesicle Detection to Enhance Breast Cancer Diagnosis

Analytical Chemistry, Год журнала: 2024, Номер 96(3), С. 1328 - 1335

Опубликована: Янв. 8, 2024

Tumor-derived small extracellular vesicles (tEVs) as potential biomarkers possess abundant surface proteins closely related to parent cells, which are crucial for noninvasive cancer diagnosis. However, tEVs exhibit phenotype heterogeneity and low abundance, posing a significant challenge multiplex detection with high sensitivity. Herein, we developed DNA gate-based exponential amplification CRISPR-Cas (DGEAC) system accurate ultrasensitive of tEVs, can greatly improve the accuracy breast (BC) Based on coexpression CD63 vascular endothelial growth factor (VEGF) BC-derived dual-aptamer-based AND gate fluorescent probe by proximity hybridization. By integrating target recognition trans-cleavage activity Cas12a, an autocatalysis-driven circuit was VEGF could avoid false negative signals from single protein or other interfering proteins. We achieved highly sensitive over linear range 1.75 × 103 3.5 108 particles/mL limit 1.02 particles/mL. Furthermore, DGEAC distinguish derived different BC cell lines, including MDA-MB-231, MCF-7, SKBR3, MCF-10A. Compared (AUC 90.0%), effectively differentiates in stages 98.3%).

Язык: Английский

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CRISPR/Cas systems accelerating the development of aptasensors

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 158, С. 116775 - 116775

Опубликована: Сен. 24, 2022

Язык: Английский

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Advances of surface-enhanced Raman spectroscopy in exosomal biomarkers analysis

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 167, С. 117253 - 117253

Опубликована: Авг. 23, 2023

Язык: Английский

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CRISPR/Cas12-based electrochemical biosensors for clinical diagnostic and food monitoring

Bioelectrochemistry, Год журнала: 2023, Номер 155, С. 108600 - 108600

Опубликована: Ноя. 5, 2023

Язык: Английский

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CRISPR/Cas12a-drived fluorescent and electrochemical signal-off/on dual-mode biosensors for ultrasensitive detection of EGFR 19del mutation

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 392, С. 134034 - 134034

Опубликована: Июнь 7, 2023

Язык: Английский

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PAM-free cascaded strand displacement coupled with CRISPR-Cas12a for amplified electrochemical detection of SARS-CoV-2 RNA

Analytical Biochemistry, Год журнала: 2023, Номер 664, С. 115046 - 115046

Опубликована: Янв. 12, 2023

Язык: Английский

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Ultrasensitive electrochemical detection of amyloid-beta oligomers using double amplification strategy by MXene substrate and covalent organic framework-based probe

Talanta, Год журнала: 2023, Номер 266, С. 125134 - 125134

Опубликована: Авг. 28, 2023

Язык: Английский

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