Advanced Sensor Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 17, 2024
Abstract
Exosomes,
secreted
by
various
types
of
cells,
play
a
crucial
role
in
cell‐to‐cell
communication
transporting
essential
molecular
cargos
that
largely
mirror
the
pathophysiological
attributes
their
parent
cells.
Increasing
evidence
has
shown
exosomes
emerge
as
reliable
early
biomarkers
for
different
diseases.
Clearly,
exosomal
detection
with
high
sensitivity
and
specificity
becomes
highly
to
advance
understanding
disease
progression
develop
diagnostic
modalities.
Among
biosensors,
aptasensors
have
received
significant
attention
tools
considering
programmability
targeting
ability.
Noble
metal
nanomaterials
possess
distinctive
physicochemical
properties,
allowing
ready
functionalization
aptamers
via
both
physical
adsorption
chemical
immobilization.
By
utilizing
recognition
elements,
noble
nanoparticle‐based
offer
promising
platform
rapid,
cost‐effective,
sensitive
situ
exosomes.
In
this
review,
progress
will
summarized
biomarker
diseases
recent
advances
use
nanoparticle/aptamer‐based
sensors
analysis
other
extracellular
vesicles.
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
ACS Nano,
Год журнала:
2024,
Номер
18(22), С. 14569 - 14582
Опубликована: Май 23, 2024
Accurately
assaying
tumor-derived
circulating
extracellular
vesicles
(EVs)
is
fundamental
in
noninvasive
cancer
diagnosis
and
therapeutic
monitoring
but
limited
by
challenges
efficient
EV
isolation
profiling.
Here,
we
report
a
bioinspired
buoyancy-driven
metal-organic
framework
(MOF)
corona
that
leverages
on-bubble
coordination
dual-encoded
surface-enhanced
Raman
scattering
(SERS)
nanotags
to
streamline
rapid
ultrasensitive
profiling
of
plasma
EVs
single
assay
for
diagnostics.
This
integrated
bubble-MOF-SERS
(IBMsv)
allows
barnacle-like
high-density
adhesion
MOFs
on
self-floating
bubble
surface
enable
fast
(2
min,
near
90%
capture
efficiency)
tumor
via
enhanced
EV-MOF
binding.
Also,
IBMsv
harnesses
four-plexed
SERS
profile
the
captured
protein
markers
at
single-particle
level.
Such
sensitive
multiplexed
across
five
types,
revealing
heterogeneous
expression
patterns.
Furthermore,
enables
pilot
clinical
cohort
(
ACS Applied Nano Materials,
Год журнала:
2024,
Номер
7(1), С. 11 - 31
Опубликована: Янв. 2, 2024
As
a
promising
spectroscopic
technique,
surface
enhanced
raman
spectroscopy
(SERS)
has
been
intensively
used
in
bio/chemical
sensing,
attributing
to
its
unique
advantages
of
ultrasensitive
and
accurate
detection
trace
amounts
analytes,
high
specific
fingerprint-like
features,
fast
response,
noninvasive
sensing.
The
robustness
consistency
SERS
signals
practical
analytical
applications
highly
rely
on
the
composition,
structural
morphology,
uniformity
substrates.
These
factors
play
pivotal
role
determining
intensity
reproducibility
detected
signals.
substrates
based
ordered
nanostructures
that
are
fabricated
from
anodic
aluminum
oxide
(AAO)-template-assisted
approaches
significant
interest
due
their
cost
effectiveness,
scalability,
precise
control,
exceptionally
features.
In
this
review,
recent
progress
with
sensitivity
prepared
AAO
templates
is
highlighted.
We
emphasize
optimization
strategies
toward
achieving
efficient
SERS-active
by
fine-tuning
size,
morphology
AAO-derived
nanostructures.
Furthermore,
we
delve
into
discussion
flexible
smart
substrates,
while
also
exploring
key
aspects
pertinent
further
amplifying
Overall,
review
aims
offer
insights
future
integration
technique
SERS,
providing
crucial
perspectives
for
forthcoming
research
field.
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 17, 2024
Cancer-derived
exosomes
carry
a
large
number
of
specific
molecular
profiles
from
cancer
cells
and
have
emerged
as
ideal
biomarkers
for
early
diagnosis.
Accurate
detection
ultralow-abundance
in
complex
biological
samples
remains
great
challenge.
Herein,
novel
SERS
aptasensor
powered
by
cascaded
signal
amplification
CRISPR/Cas13a
