Cell competition in development, homeostasis and cancer

Nature Reviews Molecular Cell Biology, Год журнала: 2022, Номер 24(3), С. 221 - 236

Опубликована: Сен. 29, 2022

Язык: Английский

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Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation

Cell stem cell, Год журнала: 2023, Номер 30(9), С. 1235 - 1245.e6

Опубликована: Сен. 1, 2023

Язык: Английский

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Generation of rat forebrain tissues in mice

Cell, Год журнала: 2024, Номер 187(9), С. 2129 - 2142.e17

Опубликована: Апрель 1, 2024

Язык: Английский

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The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras

Development, Год журнала: 2021, Номер 148(12)

Опубликована: Июнь 15, 2021

Growing human organs in animals sounds like something from the realm of science fiction, but it may one day become a reality through technique known as interspecies blastocyst complementation. This technique, which was originally developed to study gene function development, involves injecting donor pluripotent stem cells into an organogenesis-disabled host embryo, allowing compensate for missing or tissues. Although complementation has been achieved between closely related species, such mice and rats, situation becomes much more difficult species that are far apart on evolutionary tree. is presumably because layers xenogeneic barriers result divergent evolution. In this Review, we discuss current status approaches and, light recent progress, elaborate keys success organ generation.

Язык: Английский

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Reprogramming: Emerging Strategies to Rejuvenate Aging Cells and Tissues

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(8), С. 3990 - 3990

Опубликована: Апрель 13, 2021

Aging is associated with a progressive and functional decline of all tissues striking increase in many “age-related diseases”. Although aging has long been considered an inevitable process, strategies to delay potentially even reverse the process have recently developed. Here, we review emerging rejuvenation that are based on reprogramming toward pluripotency. Some these approaches may eventually lead medical applications improve healthspan longevity.

Язык: Английский

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Generation of functional chimeric kidney containing exogenous progenitor-derived stroma and nephron via a conditional empty niche

Cell Reports, Год журнала: 2022, Номер 39(11), С. 110933 - 110933

Опубликована: Июнь 1, 2022

Generation of new kidneys can be useful in various research fields, including organ transplantation. However, generating renal stroma, an important component tissue for structural support, endocrine function, and kidney development, remains difficult. Organ generation using animal developmental niche provide appropriate vivo environment stroma differentiation. Here, we generate rat with capacity by removing mouse stromal progenitor cells (SPCs) from the host transplanting SPCs. Furthermore, develop a method to replace both nephron (NPCs) SPCs, called interspecies dual replacement (i-DROP) system, successfully functional chimeric containing nephrons stroma. This progenitors reduce xenotransplant rejection. Moreover, it is safe method, as donor do not stray into nontarget organs, thus accelerating on stem cells, chimeras, xenotransplantation.

Язык: Английский

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Cell competition and the regulative nature of early mammalian development

Cell stem cell, Год журнала: 2022, Номер 29(7), С. 1018 - 1030

Опубликована: Июль 1, 2022

Язык: Английский

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Nuclear transfer and the development of genetically modified/gene edited livestock

Reproduction, Год журнала: 2021, Номер unknown

Опубликована: Май 1, 2021

The birth and adult development of “Dolly” the sheep, first mammal produced by transfer a terminally differentiated cell nucleus into an egg, provided unequivocal evidence nuclear equivalence among somatic cells. This ground-breaking experiment challenged long-standing dogma irreversible cellular differentiation that prevailed for over century enabled methodologies reversal cells, also known as reprogramming. Thanks to this new paradigm, novel alternatives regenerative medicine in humans, improved animal breeding domestic animals approaches species conservation through reproductive have emerged. Combined with incorporation tools genetic modification, these techniques promise i) transform accelerate our understanding diseases targeted therapies creation tailored models, ii) provide safe tissues organs xenotransplantation, iii) contribute preservation endangered species, iv) improve global food security whilst reducing environmental impact production. review discusses recent advances build on conceptual legacy - when combined gene editing will transformative potential medicine, biodiversity sustainable agriculture. We conclude technologies depends further fundamental translational research directed at improving efficiency safety methods.

Язык: Английский

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Xenotransplantation and interspecies organogenesis: current status and issues

Frontiers in Endocrinology, Год журнала: 2022, Номер 13

Опубликована: Авг. 5, 2022

Pancreas (and islet) transplantation is the only curative treatment for type 1 diabetes patients whose β-cell functions have been abolished. However, lack of donor organs has major hurdle to save a large number patients. Therefore, animal expected be an alternative method solve serious shortage organs. More recently, generate from pluripotent stem cells inside body other species developed. This interspecies organ generation using blastocyst complementation (BC) next-generation regenerative medicine. Here, we describe recent advances and future prospects these two approaches.

Язык: Английский

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Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells

Nature Cell Biology, Год журнала: 2023, Номер 25(12), С. 1758 - 1773

Опубликована: Ноя. 2, 2023

Abstract Skeletal muscle stem and progenitor cells including those derived from human pluripotent (hPSCs) offer an avenue towards personalized therapies readily fuse to form human–mouse myofibres in vivo. However, skeletal (SMPCs) inefficiently colonize chimeric cell niches instead associate with resembling foetal niches. We hypothesized competition mouse satellite (SCs) prevented SMPC engraftment into the SC niche thus generated ablation compatible engraftment. Single-nucleus RNA sequencing of SC-ablated mice identified absence a transient myofibre subtype during regeneration expressing Actc1 . Similarly, ACTC1 + supporting PAX7 SMPCs increased mice, after re-injury we found could now repopulate To demonstrate are essential SMPCs, caspase-inducible depletion cells, upon 90% reduction 100-fold decrease numbers compared non-induced controls. used spatial identify key factors driving emerging formation between This revealed that regenerating for vivo support SMPCs.

Язык: Английский

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