An innovative approach of bioremediation in enzymatic degradation of xenobiotics DOI

Shruti Rathore,

Ayushi Varshney, Sumedha Mohan

и другие.

Biotechnology and Genetic Engineering Reviews, Год журнала: 2022, Номер 38(1), С. 1 - 32

Опубликована: Янв. 2, 2022

Worldwide, environmental pollution due to a complex mixture of xenobiotics has become serious concern. Several xenobiotic compounds cause contamination their severe toxicity, prolonged exposure, and limited biodegradability. From the past few decades, microbial-assisted degradation (bioremediation) pollutants evolved as most effective, eco-friendly, valuable approach. Microorganisms have unique metabolism, capability genetic modification, diversity enzymes, various pathways necessary for bioremediation process. Microbial is effective but slow process that limits its application in bioremediation. However, study microbial enzymes gaining global importance. huge ability transform contaminants into non-toxic forms thereby reduce pollution. Recently, advanced techniques, including metagenomics, proteomics, transcriptomics, metabolomics are effectively utilized characterization, metabolic machinery, new proteins, genes microorganisms involved These molecular techniques provide thorough understanding structural functional aspects microorganisms. This review gives brief note on impact environment. Particular attention will be devoted class such cytochrome P450, dehydrogenase, laccase, hydrolase, protease, lipase, etc. capable converting these innocuous products. attempts deliver knowledge role biodegradation pollutants, along with use technologies like recombinant DNA technology Omics approaches make more robust effective.

Язык: Английский

Role of Cytochrome P450 Enzymes in Plant Stress Response DOI Creative Commons
Balaji Aravindhan Pandian, Rajendran Sathishraj, M. Djanaguiraman

и другие.

Antioxidants, Год журнала: 2020, Номер 9(5), С. 454 - 454

Опубликована: Май 25, 2020

Cytochrome P450s (CYPs) are the largest enzyme family involved in NADPH- and/or O2-dependent hydroxylation reactions across all domains of life. In plants and animals, CYPs play a central role detoxification xenobiotics. addition to this function, act as versatile catalysts crucial biosynthesis secondary metabolites, antioxidants, phytohormones higher plants. The molecular biochemical processes catalyzed by have been well characterized, however, relationship between process its effect on several plant functions was not established. advent next-generation sequencing opened new avenues unravel involvement such stress response. expression CYP genes regulated response environmental stresses, they also prominent crosstalk abiotic biotic responses. an enormous potential be used candidate for engineering crop species resilient stresses. objective review is summarize latest research

Язык: Английский

Процитировано

330
Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis DOI
Roger A. Sheldon, Dean Brady

ChemSusChem, Год журнала: 2019, Номер 12(13), С. 2859 - 2881

Опубликована: Апрель 1, 2019

Abstract This Review is aimed at synthetic organic chemists who may be familiar with organometallic catalysis but have no experience biocatalysis, and seeks to provide an answer the perennial question: if it so attractive, why wasn't extensively used in past? The development of biocatalysis industrial synthesis traced from middle last century. Advances molecular biology two decades, particular genome sequencing, gene directed evolution proteins, enabled remarkable improvements scope substantially reduced biocatalyst times cost contributions. Additionally, recovery reuse been facilitated by developments enzyme immobilization technologies. Biocatalysis has become eminently competitive chemocatalysis biocatalytic production important pharmaceutical intermediates, such as enantiopure alcohols amines, mainstream synthesis. space significantly expanded currently being extended even further include new‐to‐nature reactions.

Язык: Английский

Процитировано

275
The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis DOI Creative Commons
R. Ann Sheldon, Dean Brady, Moira L. Bode

и другие.

Chemical Science, Год журнала: 2020, Номер 11(10), С. 2587 - 2605

Опубликована: Янв. 1, 2020

Enzymes are excellent catalysts that increasingly being used in industry and academia. This Perspective provides a general practical guide to enzymes their synthetic potential, primarily aimed at organic chemists.

Язык: Английский

Процитировано

265
Revealing the role of adsorption in ciprofloxacin and sulfadiazine elimination routes in microalgae DOI
Peng Xie, Chuan Chen, Chaofan Zhang

и другие.

Water Research, Год журнала: 2020, Номер 172, С. 115475 - 115475

Опубликована: Янв. 6, 2020

Язык: Английский

Процитировано

238
Microbial Enzymes Used in Bioremediation DOI Creative Commons
Sobika Bhandari, Darbin Kumar Poudel, Rishab Marahatha

и другие.

Journal of Chemistry, Год журнала: 2021, Номер 2021, С. 1 - 17

Опубликована: Фев. 8, 2021

Emerging pollutants in nature are linked to various acute and chronic detriments biotic components subsequently deteriorate the ecosystem with serious hazards. Conventional methods for removing not efficient; instead, they end up formation of secondary pollutants. Significant destructive impacts perinatal disorders, mortality, respiratory allergy, cancer, cardiovascular mental other harmful effects. The pollutant substrate can recognize different microbial enzymes at optimum conditions (temperature/pH/contact time/concentration) efficiently transform them into rather unharmful products. most representative involved bioremediation include cytochrome P450s, laccases, hydrolases, dehalogenases, dehydrogenases, proteases, lipases, which have shown promising potential degradation polymers, aromatic hydrocarbons, halogenated compounds, dyes, detergents, agrochemical etc. Such is favored by mechanisms such as oxidation, reduction, elimination, ring-opening. significant be upgraded utilizing genetically engineered microorganisms that produce many recombinant through eco-friendly new technology. So far, few been exploited, vast diversity still unexplored. This review would also useful further research enhance efficiency xenobiotic pollutants, including agrochemical, microplastic, polyhalogenated hydrocarbons.

Язык: Английский

Процитировано

221
Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications DOI Creative Commons
Zhong Li, Yuanyuan Jiang, F. Peter Guengerich

и другие.

Journal of Biological Chemistry, Год журнала: 2020, Номер 295(3), С. 833 - 849

Опубликована: Янв. 1, 2020

Cytochrome P450 enzymes (P450s) are broadly distributed among living organisms and play crucial roles in natural product biosynthesis, degradation of xenobiotics, steroid drug metabolism. P450s considered as the most versatile biocatalysts nature because vast variety substrate structures types reactions they catalyze. In particular, can catalyze regio- stereoselective oxidations nonactivated C–H bonds complex organic molecules under mild conditions, making useful production commodity pharmaceuticals, fine or bulk chemicals, bioremediation agents, flavors, fragrances. Major efforts have been made engineering improved systems that overcome inherent limitations native enzymes. this review, we focus on recent progress different strategies, including protein engineering, redox-partner electron source P450-mediated metabolic to more efficiently produce pharmaceuticals other chemicals. We also discuss future opportunities for applications systems.

Язык: Английский

Процитировано

199
Biotransformation of sulfamethoxazole by microalgae: Removal efficiency, pathways, and mechanisms DOI

Yuhao Chu,

Chaofan Zhang, Rupeng Wang

и другие.

Water Research, Год журнала: 2022, Номер 221, С. 118834 - 118834

Опубликована: Июль 7, 2022

Язык: Английский

Процитировано

148
Polyunsaturated Fatty Acids: Conversion to Lipid Mediators, Roles in Inflammatory Diseases and Dietary Sources DOI Open Access
John L. Harwood

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8838 - 8838

Опубликована: Май 16, 2023

Polyunsaturated fatty acids (PUFAs) are important components of the diet mammals. Their role was first established when essential (EFAs) linoleic acid and α-linolenic were discovered nearly a century ago. However, most biochemical physiological actions PUFAs rely on their conversion to 20C or 22C subsequent metabolism lipid mediators. As generalisation, mediators formed from n-6 pro-inflammatory while those n-3 anti-inflammatory neutral. Apart classic eicosanoids docosanoids, many newly compounds described as Specialised Pro-resolving Mediators (SPMs) which have been proposed in resolving inflammatory conditions such infections preventing them becoming chronic. In addition, large group molecules, termed isoprostanes, can be generated by free radical reactions these too powerful properties towards inflammation. The ultimate source photosynthetic organisms contain Δ-12 Δ-15 desaturases, almost exclusively absent animals. Moreover, EFAs consumed plant food competition with each other for Thus, relative amounts important. Furthermore, mammals is rather poor. there has much interest recently use algae, make substantial quantities long-chain manipulating oil crops acids. This especially because fish oils, main human diets, limited. this review, metabolic into different described. Then, biological roles molecular mechanisms diseases outlined. Finally, natural sources (including 20 22 carbon compounds) detailed, well recent efforts increase production.

Язык: Английский

Процитировано

55
Expanding chemistry through in vitro and in vivo biocatalysis DOI
Elijah N. Kissman, Max B. Sosa,

Douglas C Millar

и другие.

Nature, Год журнала: 2024, Номер 631(8019), С. 37 - 48

Опубликована: Июль 3, 2024

Язык: Английский

Процитировано

33
Engineering Hydroxylase Activity, Selectivity, and Stability for a Scalable Concise Synthesis of a Key Intermediate to Belzutifan DOI
Wai Ling Cheung‐Lee, Joshua N. Kolev, John A. McIntosh

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(13)

Опубликована: Янв. 27, 2024

Biocatalytic oxidations are an emerging technology for selective C-H bond activation. While promising a range of oxidations, practical use enzymes catalyzing aerobic hydroxylation is presently limited by their substrate scope and stability under industrially relevant conditions. Here, we report the engineering application non-heme iron α-ketoglutarate-dependent dioxygenase direct stereo- regio-selective non-native fluoroindanone en route to oncology treatment belzutifan, replacing five-step chemical synthesis with enantioselective hydroxylation. Mechanistic studies indicated that formation desired product was enzyme overoxidation, these properties subsequently improved directed evolution, yielding biocatalyst capable >15,000 total turnovers. Highlighting industrial utility this biocatalyst, high-yielding, green, efficient oxidation demonstrated at kilogram scale belzutifan.

Язык: Английский

Процитировано

30