Nanomaterials-integrated
CRISPR/Cas
systems
have
rapidly
emerged
as
powerful
next-generation
platforms
for
optical
biosensing.
These
integrated
harness
the
precision
of
CRISPR/Cas-mediated
nucleic
acid
detection
while
leveraging
unique
properties
nanomaterials
to
achieve
enhanced
sensitivity
and
expanded
analytical
capabilities,
thereby
broadening
their
diagnostic
potential.
By
incorporating
a
diverse
range
nanomaterials,
these
effectively
expand
toolbox
detection,
offering
adaptable
solutions
tailored
various
challenges.
This
review
provides
comprehensive
overview
successfully
into
CRISPR/Cas-based
sensing
systems.
It
examines
multiple
modalities,
including
fluorescence,
electrochemiluminescence,
colorimetry,
surface-enhanced
Raman
spectroscopy,
highlighting
how
facilitate
signal
amplification,
enable
multiplexing,
support
development
point-of-care
applications.
Additionally,
practical
applications
in
critical
fields
such
healthcare
diagnostics
environmental
monitoring
are
showcased.
While
offer
considerable
advantages,
several
real-world
challenges
complexity
assay
workflows,
impact
cost,
regulatory
hurdles
must
be
addressed
before
widespread
implementation
can
achieved.
identifying
obstacles
proposing
strategic
solutions,
we
aim
pave
way
continued
advancement
adoption
nanomaterial-integrated
biosensing
technologies.
Nano Letters,
Год журнала:
2024,
Номер
24(7), С. 2360 - 2368
Опубликована: Фев. 12, 2024
Accurate
and
sensitive
analysis
of
circulating
tumor
cells
(CTCs)
in
human
blood
provides
a
non-invasive
approach
for
the
evaluation
cancer
metastasis
early
diagnosis.
Herein,
we
demonstrate
controllable
assembly
quantum
dot
(QD)-based
aptasensor
guided
by
CRISPR/Cas12a
direct
measurement
CTCs
blood.
We
introduce
magnetic
bead@activator/recognizer
duplex
core–shell
structure
to
construct
multifunctional
platform
capture
detection
blood,
without
need
additional
CTC
release
re-identification
steps.
Notably,
introduction
separation
ensures
that
only
target-induced
free
activator
can
initiate
downstream
catalysis,
efficiently
avoiding
undesired
catalysis
triggered
inappropriate
recognition
activator/recognizer
crRNAs.
This
achieves
high
CTC-capture
efficiency
(82.72%)
with
limit
2
mL–1
holding
great
promise
liquid
biopsy
cancers.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(22), С. 7848 - 7948
Опубликована: Янв. 1, 2023
Nanoscale
dye-based
excitonic
systems
assembled
on
DNA
origami
in
solution
excited
by
a
laser.
Dyes
engage
cascaded
FRET
with
exciton
movement
guided
programmed
elements
engaging
homo-
and
hetero-energy
transfer.
Lab on a Chip,
Год журнала:
2023,
Номер
23(6), С. 1467 - 1492
Опубликована: Янв. 1, 2023
Critical
development
of
CRISPR-based
diagnostics
coupled
with
nucleic
acid
amplification
and
amplification-free
techniques;
various
purposes
CRISPR
including
determination,
quantification,
multiplexed
point-of-care
diagnostics.
Nano Letters,
Год журнала:
2023,
Номер
24(1), С. 202 - 208
Опубликована: Дек. 21, 2023
This
work
presents
a
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/Cas-nanopipette
nano-electrochemistry
(Cas
=
CRISPR-associated
proteins)
capable
of
ultrasensitive
microRNA
detection.
Nanoconfinement
the
CRISPR/Cas13a
within
nanopipette
leads
to
high
catalytic
efficacy
ca.
169
times
higher
than
that
in
bulk
electrolyte,
contributing
amplified
electrochemical
responses.
CRISPR/Cas13a-enabled
detection
representative
microRNA-25
achieves
low
limit
down
10
aM.
Practical
application
this
method
is
further
demonstrated
for
single-cell
and
real
human
serum
Its
general
applicability
validated
by
addressing
microRNA-141
SARS-CoV-2
RNA
gene
fragment.
introduces
new
CRISPR/Cas-empowered
nanotechnology
bioanalysis.
