Fluorescence coding techniques for RNA detection DOI Creative Commons

Junren Wang,

Qin Xiang, Haifeng Dong

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(6), P. 914 - 932

Published: Jan. 1, 2024

Schematic illustration of fluorescence coding techniques in RNA detection.

Language: Английский

Controllable Assembly of a Quantum Dot-Based Aptasensor Guided by CRISPR/Cas12a for Direct Measurement of Circulating Tumor Cells in Human Blood DOI
Qian Zhang, Xin Gao, Yi‐Ping Ho

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(7), P. 2360 - 2368

Published: Feb. 12, 2024

Accurate and sensitive analysis of circulating tumor cells (CTCs) in human blood provides a non-invasive approach for the evaluation cancer metastasis early diagnosis. Herein, we demonstrate controllable assembly quantum dot (QD)-based aptasensor guided by CRISPR/Cas12a direct measurement CTCs blood. We introduce magnetic bead@activator/recognizer duplex core–shell structure to construct multifunctional platform capture detection blood, without need additional CTC release re-identification steps. Notably, introduction separation ensures that only target-induced free activator can initiate downstream catalysis, efficiently avoiding undesired catalysis triggered inappropriate recognition activator/recognizer crRNAs. This achieves high CTC-capture efficiency (82.72%) with limit 2 mL–1 holding great promise liquid biopsy cancers.

Language: Английский

Citations

32
CRISPR/Cas12a-derived ratiometric fluorescence sensor for high-sensitive Pb2+ detection based on CDs@ZIF-8 and DNAzyme DOI

Chuanyu Yang,

Caiyi Du,

Feiyu Yuan

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 251, P. 116089 - 116089

Published: Feb. 3, 2024

Language: Английский

Citations

23
Pursuing excitonic energy transfer with programmable DNA-based optical breadboards DOI Creative Commons
Divita Mathur, Sebastián A. Dı́az, Niko Hildebrandt

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(22), P. 7848 - 7948

Published: Jan. 1, 2023

Nanoscale dye-based excitonic systems assembled on DNA origami in solution excited by a laser. Dyes engage cascaded FRET with exciton movement guided programmed elements engaging homo- and hetero-energy transfer.

Language: Английский

Citations

25
Recent progress in nucleic acid detection with CRISPR DOI Open Access

Frank X. Liu,

Johnson Q. Cui, Zhihao Wu

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(6), P. 1467 - 1492

Published: Jan. 1, 2023

Critical development of CRISPR-based diagnostics coupled with nucleic acid amplification and amplification-free techniques; various purposes CRISPR including determination, quantification, multiplexed point-of-care diagnostics.

Language: Английский

Citations

23
Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection DOI

Xueying Lei,

Shengnan Cao, Tao Liu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125663 - 125663

Published: Jan. 16, 2024

Language: Английский

Citations

12
Signal-On Aptasensors on Paper-Based Platform: Application of Multilayer MXene Nanoquencher and Stabilized Luminescent Carbon Dots DOI
Jincai Yang, Mingzhu Liu, Jin Wu

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137720 - 137720

Published: Feb. 23, 2025

Language: Английский

Citations

1
CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases DOI Creative Commons
Yawen He, Qinqin Hu,

Samantha San

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 168, P. 117342 - 117342

Published: Sept. 30, 2023

Language: Английский

Citations

18
One-step self-assembly of quantum dot-based spherical nucleic acid nanostructure for accurate monitoring of long noncoding RNA MALAT1 in living cells and tissues DOI
Qian Zhang, Ran Zhao, Yan Zhang

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 469, P. 144021 - 144021

Published: June 9, 2023

Language: Английский

Citations

14
An Ultrasensitive and Efficient microRNA Nanosensor Empowered by the CRISPR/Cas Confined in a Nanopore DOI
Bing Wang,

Yi‐Tong Xu,

Tianyang Zhang

et al.

Nano Letters, Journal Year: 2023, Volume and Issue: 24(1), P. 202 - 208

Published: Dec. 21, 2023

This work presents a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas-nanopipette nano-electrochemistry (Cas = CRISPR-associated proteins) capable of ultrasensitive microRNA detection. Nanoconfinement the CRISPR/Cas13a within nanopipette leads to high catalytic efficacy ca. 169 times higher than that in bulk electrolyte, contributing amplified electrochemical responses. CRISPR/Cas13a-enabled detection representative microRNA-25 achieves low limit down 10 aM. Practical application this method is further demonstrated for single-cell and real human serum Its general applicability validated by addressing microRNA-141 SARS-CoV-2 RNA gene fragment. introduces new CRISPR/Cas-empowered nanotechnology bioanalysis.

Language: Английский

Citations

14
Recent advances of nanoparticles-assisted CRISPR/Cas biosensors DOI
Sitong Liu, Xu Li,

Zhaohe Huang

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 199, P. 109930 - 109930

Published: Jan. 9, 2024

Language: Английский

Citations

6