Microchemical Journal, Год журнала: 2021, Номер 167, С. 106305 - 106305
Опубликована: Апрель 19, 2021
Язык: Английский
Talanta, Год журнала: 2021, Номер 240, С. 123197 - 123197
Опубликована: Дек. 31, 2021
Язык: Английский
Процитировано
64
Bioelectrochemistry, Год журнала: 2022, Номер 146, С. 108105 - 108105
Опубликована: Март 20, 2022
Язык: Английский
Процитировано
61
Advanced Materials Technologies, Год журнала: 2021, Номер 6(12)
Опубликована: Июль 19, 2021
CRISPR (Clustered regularly interspaced short palindromic repeats)-based diagnostic technologies have emerged as a promising alternative to accelerate delivery of SARS-CoV-2 molecular detection at the point need. However, efficient translation CRISPR-diagnostic field application is still hampered by dependence on target amplification and reliance fluorescence-based results readout. Herein, an amplification-free CRISPR/Cas12a-based technology for RNA presented using smartphone camera This method, termed Cellphone-based system with CRISPR/CAS-dependent enzymatic (CASCADE) assay, relies mobile phone imaging catalase-generated gas bubble signal within microfluidic channel does not require any external hardware optical attachments. Upon specific reverse-transcribed DNA/RNA heteroduplex (orf1ab) ribonucleoprotein complex, transcleavage collateral activity Cas12a protein Catalase:ssDNA probe triggers system. High analytical sensitivity in without previous (down 50 copies µL
Язык: Английский
Процитировано
59
Sensors and Actuators B Chemical, Год журнала: 2021, Номер 354, С. 131228 - 131228
Опубликована: Дек. 9, 2021
Язык: Английский
Процитировано
59
Small Methods, Год журнала: 2022, Номер 6(10)
Опубликована: Сен. 16, 2022
Abstract Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, accurate detection of is importance for preventing infectious diseases caused by optimizing medical healthcare systems. Inspired a microbial defense system (i.e., CRISPR/ CRISPR‐associated proteins (Cas) system, an adaptive immune protecting microorganisms from being attacked invading species), many new biosensors have been successfully developed widely applied in the viruses pathogenic bacteria. Moreover, advanced nanotechnologies also integrated into these to improve their stability, sensitivity, accuracy. In this review, recent advance CRISPR/Cas systems‐based nano/biosensors applications bacteria are comprehensively reviewed. First all, categories working principles systems establishing simply introduced. Then, design construction discussed. end, attentions focused on Impressively, remaining opportunities challenges further development system‐based promising proposed.
Язык: Английский
Процитировано
48
ACS Applied Bio Materials, Год журнала: 2022, Номер 5(6), С. 2431 - 2460
Опубликована: Май 18, 2022
The COVID-19 pandemic caused by the SARS-CoV-2, a ribonucleic acid (RNA) virus that emerged less than two years ago but has nearly 6.1 million deaths to date. Recently developed variants of SARS-CoV-2 have been shown be more potent and expanded at faster rate. Until now, there is no specific effective treatment for in terms reliable sustainable recovery. Precaution, prevention, vaccinations are only ways keep situation under control. Medical scientific professionals now focusing on repurposing previous technology trying develop fruitful methodologies detect presence viruses, treat patients, precautionary items, vaccine developments. Nanomedicine or nanobased platforms can play crucial role these fronts. Researchers working many approaches nanosized particles combat SARS-CoV-2. platform extremely diverse (i.e., mark personal protective suit, rapid diagnostic tool targeted treatment, developments). Although theoretical possibilities until an inadequate number research targeting explore such scenarios. This unique mini-review aims compile elaborate recent advances from diagnostics, developments against associated challenges.
Язык: Английский
Процитировано
46
BioChip Journal, Год журнала: 2022, Номер 16(4), С. 376 - 396
Опубликована: Авг. 10, 2022
Язык: Английский
Процитировано
46
ACS Central Science, Год журнала: 2023, Номер 9(4), С. 657 - 667
Опубликована: Март 15, 2023
Nucleic acid detection methods based on CRISPR and isothermal amplification techniques show great potential for point-of-care diagnostic applications. However, most current rely fluorescent or lateral flow assay readout, requiring external excitation postamplification reaction transfer. Here, we developed a bioluminescent nucleic sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by pair of dCas9-based probes mediating split NanoLuc luciferase complementation. LUNAS easily integrated with recombinase polymerase (RPA), providing attomolar sensitivity rapid one-pot assay. A calibrator included robust ratiometric enabling real-time monitoring the RPA using simple digital camera. We designed an RT-RPA-LUNAS that allows SARS-CoV-2 RNA without need cumbersome isolation demonstrated its performance COVID-19 patient nasopharyngeal swab samples. Detection from samples viral loads ∼200 cp/μL was achieved within ∼20 min, showing RPA-LUNAS attractive infectious disease testing.
Язык: Английский
Процитировано
32
Analytica Chimica Acta, Год журнала: 2023, Номер 1265, С. 341278 - 341278
Опубликована: Апрель 30, 2023
Язык: Английский
Процитировано
28
Lab on a Chip, Год журнала: 2023, Номер 23(6), С. 1467 - 1492
Опубликована: Янв. 1, 2023
Critical development of CRISPR-based diagnostics coupled with nucleic acid amplification and amplification-free techniques; various purposes CRISPR including determination, quantification, multiplexed point-of-care diagnostics.
Язык: Английский
Процитировано
23