Microchemical Journal, Journal Year: 2021, Volume and Issue: 167, P. 106305 - 106305
Published: April 19, 2021
Language: Английский
Talanta, Journal Year: 2021, Volume and Issue: 240, P. 123197 - 123197
Published: Dec. 31, 2021
Language: Английский
Citations
64
Bioelectrochemistry, Journal Year: 2022, Volume and Issue: 146, P. 108105 - 108105
Published: March 20, 2022
Language: Английский
Citations
61
Advanced Materials Technologies, Journal Year: 2021, Volume and Issue: 6(12)
Published: July 19, 2021
CRISPR (Clustered regularly interspaced short palindromic repeats)-based diagnostic technologies have emerged as a promising alternative to accelerate delivery of SARS-CoV-2 molecular detection at the point need. However, efficient translation CRISPR-diagnostic field application is still hampered by dependence on target amplification and reliance fluorescence-based results readout. Herein, an amplification-free CRISPR/Cas12a-based technology for RNA presented using smartphone camera This method, termed Cellphone-based system with CRISPR/CAS-dependent enzymatic (CASCADE) assay, relies mobile phone imaging catalase-generated gas bubble signal within microfluidic channel does not require any external hardware optical attachments. Upon specific reverse-transcribed DNA/RNA heteroduplex (orf1ab) ribonucleoprotein complex, transcleavage collateral activity Cas12a protein Catalase:ssDNA probe triggers system. High analytical sensitivity in without previous (down 50 copies µL
Language: Английский
Citations
59
Sensors and Actuators B Chemical, Journal Year: 2021, Volume and Issue: 354, P. 131228 - 131228
Published: Dec. 9, 2021
Language: Английский
Citations
59
Small Methods, Journal Year: 2022, Volume and Issue: 6(10)
Published: Sept. 16, 2022
Abstract Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, accurate detection of is importance for preventing infectious diseases caused by optimizing medical healthcare systems. Inspired a microbial defense system (i.e., CRISPR/ CRISPR‐associated proteins (Cas) system, an adaptive immune protecting microorganisms from being attacked invading species), many new biosensors have been successfully developed widely applied in the viruses pathogenic bacteria. Moreover, advanced nanotechnologies also integrated into these to improve their stability, sensitivity, accuracy. In this review, recent advance CRISPR/Cas systems‐based nano/biosensors applications bacteria are comprehensively reviewed. First all, categories working principles systems establishing simply introduced. Then, design construction discussed. end, attentions focused on Impressively, remaining opportunities challenges further development system‐based promising proposed.
Language: Английский
Citations
48
ACS Applied Bio Materials, Journal Year: 2022, Volume and Issue: 5(6), P. 2431 - 2460
Published: May 18, 2022
The COVID-19 pandemic caused by the SARS-CoV-2, a ribonucleic acid (RNA) virus that emerged less than two years ago but has nearly 6.1 million deaths to date. Recently developed variants of SARS-CoV-2 have been shown be more potent and expanded at faster rate. Until now, there is no specific effective treatment for in terms reliable sustainable recovery. Precaution, prevention, vaccinations are only ways keep situation under control. Medical scientific professionals now focusing on repurposing previous technology trying develop fruitful methodologies detect presence viruses, treat patients, precautionary items, vaccine developments. Nanomedicine or nanobased platforms can play crucial role these fronts. Researchers working many approaches nanosized particles combat SARS-CoV-2. platform extremely diverse (i.e., mark personal protective suit, rapid diagnostic tool targeted treatment, developments). Although theoretical possibilities until an inadequate number research targeting explore such scenarios. This unique mini-review aims compile elaborate recent advances from diagnostics, developments against associated challenges.
Language: Английский
Citations
46
BioChip Journal, Journal Year: 2022, Volume and Issue: 16(4), P. 376 - 396
Published: Aug. 10, 2022
Language: Английский
Citations
46
ACS Central Science, Journal Year: 2023, Volume and Issue: 9(4), P. 657 - 667
Published: March 15, 2023
Nucleic acid detection methods based on CRISPR and isothermal amplification techniques show great potential for point-of-care diagnostic applications. However, most current rely fluorescent or lateral flow assay readout, requiring external excitation postamplification reaction transfer. Here, we developed a bioluminescent nucleic sensor (LUNAS) platform in which target dsDNA is sequence-specifically detected by pair of dCas9-based probes mediating split NanoLuc luciferase complementation. LUNAS easily integrated with recombinase polymerase (RPA), providing attomolar sensitivity rapid one-pot assay. A calibrator included robust ratiometric enabling real-time monitoring the RPA using simple digital camera. We designed an RT-RPA-LUNAS that allows SARS-CoV-2 RNA without need cumbersome isolation demonstrated its performance COVID-19 patient nasopharyngeal swab samples. Detection from samples viral loads ∼200 cp/μL was achieved within ∼20 min, showing RPA-LUNAS attractive infectious disease testing.
Language: Английский
Citations
32
Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1265, P. 341278 - 341278
Published: April 30, 2023
Language: Английский
Citations
28
Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(6), P. 1467 - 1492
Published: Jan. 1, 2023
Critical development of CRISPR-based diagnostics coupled with nucleic acid amplification and amplification-free techniques; various purposes CRISPR including determination, quantification, multiplexed point-of-care diagnostics.
Language: Английский
Citations
23