Ultrasensitive detection platform for Staphylococcus aureus based on DNAzyme tandem blocking CRISPR/Cas12a system

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116671 - 116671

Published: Aug. 17, 2024

Language: Английский

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Next-generation pathogen detection: Exploring the power of nucleic acid amplification-free biosensors

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 513, P. 215895 - 215895

Published: May 4, 2024

Language: Английский

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Detection of Tetracycline with a CRISPR/Cas12a Aptasensor Using a Highly Efficient Fluorescent Polystyrene Microsphere Reporter System

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 2166 - 2176

Published: June 12, 2024

CRISPR-based diagnostics use the CRISPR-Cas system

Language: Английский

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SEDphone: Spatial encoding of centrifugal microfluidic disc integrated smartphone-controlled platform via RT/LAMP-CRISPR/Cas12a system for influenza virus subtypes detection

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 417, P. 136196 - 136196

Published: June 25, 2024

Language: Английский

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Advances in surface-enhanced Raman scattering detection of foodborne pathogens: From recognition-based fingerprint to molecular diagnosis

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 518, P. 216083 - 216083

Published: July 23, 2024

Language: Английский

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Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(11), P. 5665 - 5682

Published: Nov. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Language: Английский

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A Thermostable Cas12b-Powered Bioassay Coupled with Loop-Mediated Isothermal Amplification in a Customized “One-Pot” Vessel for Visual, Rapid, Sensitive, and On-Site Detection of Genetically Modified Crops

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 11195 - 11204

Published: April 2, 2024

Genetically modified crops (GMCs) have been discussed due to unknown safety, and thus, it is imperative develop an effective detection technology. CRISPR/Cas deemed a burgeoning technology for nucleic acid detection. Herein, we developed novel method the first time, which combined thermostable Cas12b with loop-mediated isothermal amplification (LAMP), detect genetically (GM) soybeans in customized one-pot vessel. In our method, LAMP-specific primers were used amplify cauliflower mosaic virus 35S promoter (CaMV35S) of GM soybean samples. The corresponding amplicons activated

Language: Английский

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One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(11), P. 1410 - 1426

Published: July 20, 2024

Language: Английский

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CRISPR-Cas based diagnostic tools: Bringing diagnosis out of labs

Diagnostic Microbiology and Infectious Disease, Journal Year: 2024, Volume and Issue: 109(2), P. 116252 - 116252

Published: March 8, 2024

Language: Английский

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Amplification-free nucleic acids detection with next-generation CRISPR/dx systems

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 28

Published: Sept. 22, 2024

CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite great potential CRISPR/Dx in diagnoses biologically complex diseases, preamplification template often is required for sensitive detection low-abundance nucleic acids. Various amplification-free systems were recently developed to enhance signal at sufficient sensitivity. Broadly, these are classified into five groups depending on enhancement strategies employed: CRISPR/Cas12a and/or CRISPR/Cas13a integrated with: (1) other catalytic enzymes (Cas14a, Csm6, Argonaute, duplex-specific nuclease, nanozyme, or T7 exonuclease), (2) rational-designed oligonucleotides (multivalent aptamer, tetrahedral DNA framework, RNA G-quadruplexes, roller machine, switchable-caged guide RNA, hybrid locked RNA/DNA probe, hybridized cascade "U" rich stem-loop RNA), (3) nanomaterials (nanophotonic structure, gold nanoparticle, micromotor, microbeads), (4) electrochemical piezoelectric plate biosensors (SERS nanoprobes, graphene field-effect transistor, redox primer exchange reaction), (5) cutting-edge technology platforms (digital bioanalysis, droplet microfluidic, smartphone camera, single nanoparticle counting). Herein, we critically discuss advances, pitfalls future perspectives acids detection. The continued refinement will pave road rapid, cost-effective, ultrasensitive, ultraspecific on-site without resorting target amplification, with ultimate goal establishing as paragon

Language: Английский

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