Science Bulletin, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Journal of the American Chemical Society, Год журнала: 2023, Номер 145(30), С. 16771 - 16777
Опубликована: Июль 13, 2023
DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method linking well-characterized DNA-AgNC molecules interest is presented. Three different peptides small protein, human insulin, were tested as labeling targets. The target compounds was verified by MS, HPLC, time-resolved anisotropy measurements. Moreover, spectroscopic properties DNA-AgNCs found be unaffected reactions. For DNA-AgNC-conjugated fluorescence imaging studies performed on Chinese hamster ovary (CHO) cells overexpressing insulin receptor B (hIR-B). specific staining CHO cell membranes demonstrates that great candidates applications, proposed strategy easy implement when structure known.
Язык: Английский
Процитировано
28
Nature Nanotechnology, Год журнала: 2024, Номер 19(6), С. 810 - 817
Опубликована: Фев. 13, 2024
Язык: Английский
Процитировано
17
Chemical Communications, Год журнала: 2024, Номер 60(24), С. 3315 - 3318
Опубликована: Янв. 1, 2024
DNA-stabilized silver nanoclusters with 10 to 30 atoms are interesting biocompatible nanomaterials intriguing fluorescence properties. However, they not well understood, since atom-scale high level theoretical calculations have been possible due a lack of firm experimental structural information. Here, by using density functional theory (DFT), we study the recently atomically resolved (DNA)
Язык: Английский
Процитировано
12
ACS Nano, Год журнала: 2024, Номер 18(4), С. 3002 - 3010
Опубликована: Янв. 16, 2024
Silver cations can mediate base pairing of guanine (G) DNA oligomers, yielding linear parallel G–Ag+–G duplexes with enhanced stabilities compared to those canonical duplexes. To enable their use in programmable nanotechnologies, it is critical understand solution-state formation and the nanomechanical stiffness Using temperature-controlled circular dichroism (CD) spectroscopy, we find that heating mixtures G oligomers silver salt above 50 °C fully destabilizes G-quadruplex structures converts Electrospray ionization mass spectrometry supports form at stoichiometries 1 Ag+ per pair, CD spectroscopy suggests as Ag+/base stoichiometry increases further, undergo additional morphological changes. liquid-phase atomic force microscopy, this excess enables assembly long fiberlike ∼2.5 nm heights equivalent a single duplex but lengths far exceed duplex. Finally, using conditions established duplexes, surface forces apparatus (SFA) compare solution-phase dG-dC Watson–Crick–Franklin SFA shows are 1.3 times stiffer than confirming gas-phase ion mobility measurements computational predictions. These findings may guide development structural nanotechnologies rely on silver-mediated pairing.
Язык: Английский
Процитировано
10
Nanoscale, Год журнала: 2024, Номер 16(21), С. 10087 - 10107
Опубликована: Янв. 1, 2024
In the dynamic landscape of nanotechnology, atomically precise silver nanoclusters (Ag NCs) have emerged as a novel and promising category materials with their fascinating properties enormous potential. However, recent research endeavors surged towards stabilizing Ag-based NCs, leading to innovative strategies like connecting cluster nodes organic linkers construct hierarchical structures, thus forming cluster-assembled (CAMs). This approach not only enhances structural stability, but also unveils unprecedented opportunities for CAMs, overcoming limitations individual Ag NCs. this context, review delves into captivating realm nitrogen-based ligand bonded Ag(I)-based providing insights synthetic strategies, structure-property relationships, diverse applications. We navigate challenges advancements in integrating Ag(I) nodes, bound by argentophilic interactions, highly connected periodic frameworks different dimensionalities using linkers. Despite inherent diversity among CAMs demonstrate potential sensing, catalysis, bio-imaging, device fabrication, which all are discussed review. Therefore, gaining insight nanocluster assembly process will offer valuable information, can enlighten readers on design advancement state-of-the-art
Язык: Английский
Процитировано
9
The Journal of Physical Chemistry Letters, Год журнала: 2023, Номер 14(17), С. 4078 - 4083
Опубликована: Апрель 25, 2023
Due to desirable optical properties, such as efficient luminescence and large Stokes shift, DNA-templated silver nanoclusters (DNA-AgNCs) have received significant attention over the past decade. Nevertheless, excited-state dynamics of these systems are poorly understood, studies processes ultimately leading a fluorescent state scarce. Here we investigate early time relaxation 16-atom cluster (DNA-Ag16NC) featuring NIR emission in combination with an unusually shift 5000 cm–1. We follow photoinduced DNA-Ag16NC on ranges from tens femtoseconds nanoseconds using ultrafast spectroscopies, extract kinetic model clarify physical picture dynamics. expect obtained contribute guiding research efforts toward elucidating electronic structure novel objects their potential applications fluorescence-based labeling, imaging, sensing.
Язык: Английский
Процитировано
17
TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 170, С. 117443 - 117443
Опубликована: Ноя. 18, 2023
Язык: Английский
Процитировано
17
Analytical Chemistry, Год журнала: 2024, Номер 96(8), С. 3480 - 3488
Опубликована: Фев. 14, 2024
To address the limitations of typical hairpin-structural molecular beacons, exploring ability a quasi-molecular beacon (qMB) to create label-free fluorescence biosensors is intriguing and remains challenge. Herein, we propose first example modular qMB with feature stimulation-responsive conformation switch develop an aggregated Ag nanocluster (aAgNC) in bifurcated DNA scaffold for fluorescently sensing specific initiator (I*). This was well designed program four functional modules: I*-recognizable element adopting metastable stem-loop bihairpin structure two splits (exposed C3GT4 locked C4AC4T) aAgNC template that separated by tunable hairpin spacer customized combination selective recognition signaling readout. When presenting I* assay route, hybridization induces directional disassembly unit, on which configurationally switched liberate split. Thus, parent pair C3GT4/C4AC4T proximal, affording situ nucleation clustering emissive aAgNC. By collecting signal, quantitative detection achieved. Benefiting from ingenious programming qMB, recognizing integration actuates construction facile convenient fluorescent biosensor featuring rapid reaction kinetics, wide linear range, high sensitivity, specificity. would provide new paradigm exploit versatile qMB-based biosensing platforms via switches developing various DNA-scaffolded clusters.
Язык: Английский
Процитировано
8
Nanoscale, Год журнала: 2024, Номер 16(26), С. 12329 - 12344
Опубликована: Янв. 1, 2024
Achieving atomic precision in nanostructured materials is essential for comprehending formation mechanisms and elucidating structure-property relationships. Within the realm of nanoscience technology, atomically precise ligand-protected noble metal nanoclusters (NCs) have emerged as a rapidly expanding area interest. These clusters manifest quantum confinement-induced optoelectronic, photophysical, chemical properties, along with remarkable catalytic capabilities. Among coinage metals, silver distinguishes itself fabrication stable nanoclusters, primarily due to its cost-effectiveness compared gold. This minireview provides an overview recent advancements since 2020 synthetic methodologies ligand selections toward attaining NCs boasting minimum two free valence electrons. Additionally, it explores strategies fine-tuning optical properties. The discussion extends surface reactivity, how exposure ligands, heat, light induces transformations size structure. Of paramount significance are applications reactions energy conversion, supplemented by in-depth mechanistic insights. Furthermore, review delineates challenges outlines future directions NC field, eye design new functional prospective diverse technologies, including optoelectronics, fine synthesis.
Язык: Английский
Процитировано
7
ACS Nano, Год журнала: 2024, Номер unknown
Опубликована: Сен. 24, 2024
Atomically precise metal nanoclusters (MNCs) represent a fascinating class of ultrasmall nanoparticles with molecule-like properties, bridging conventional metal-ligand complexes and nanocrystals. Despite their potential for various applications, synthesis challenges such as understanding varied synthetic parameters property-driven persist, hindering full exploitation wider application. Incorporating smart methodologies, including closed-loop framework automation, data interpretation, feedback from AI, offers promising solutions to address these challenges. In this perspective, we summarize the that has been demonstrated in nanomaterials explore research frontiers MNCs. Moreover, perspectives on inherent opportunities MNCs are discussed, aiming provide insights directions future advancements emerging field AI Science, while integration deep learning algorithms stands substantially enrich by offering enhanced predictive capabilities, optimization strategies, control mechanisms, thereby extending MNC synthesis.
Язык: Английский
Процитировано
7