Advanced Optical Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 16, 2025
Abstract
DNA‐stabilized
silver
nanoclusters
(DNA‐Ag
N
)
are
atomically
precise
and
sequence‐tuned
nanomaterials
with
potential
applications
for
deep
tissue
biomedical
imaging.
Here,
a
dual‐emissive
DNA‐Ag
is
presented
fluorescence
in
the
first
near‐infrared
(NIR‐I)
spectral
window
microsecond‐lived
photoluminescence
second
(NIR‐II)
window.
High‐resolution
electrospray
ionization
mass
spectrometry
showed
that
emitter
has
molecular
formula
(DNA)
2
[Ag
17
]
11+
.
The
crystallization
of
was
unsuccessful,
which
prevented
use
X‐ray
diffraction
to
determine
its
structure.
However,
sequence
variations
templating
DNA
oligomer
provided
insights
into
nucleobases
critical
stabilizing
Ag
Moreover,
addition
an
adenosine
or
thymidine
at
5′‐end
strand
maintained
composition
photophysical
properties
,
suggesting
site
conjugation
biomolecules
enable
targeted
labeling
future
bioimaging
applications.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(30), P. 16771 - 16777
Published: July 13, 2023
DNA-stabilized
silver
nanoclusters
(DNA-AgNCs)
are
biocompatible
emitters
with
intriguing
properties.
However,
they
have
not
been
extensively
used
for
bioimaging
applications
due
to
the
lack
of
structural
information
and
hence
predictable
conjugation
strategies.
Here,
a
copper-free
click
chemistry
method
linking
well-characterized
DNA-AgNC
molecules
interest
is
presented.
Three
different
peptides
small
protein,
human
insulin,
were
tested
as
labeling
targets.
The
target
compounds
was
verified
by
MS,
HPLC,
time-resolved
anisotropy
measurements.
Moreover,
spectroscopic
properties
DNA-AgNCs
found
be
unaffected
reactions.
For
DNA-AgNC-conjugated
fluorescence
imaging
studies
performed
on
Chinese
hamster
ovary
(CHO)
cells
overexpressing
insulin
receptor
B
(hIR-B).
specific
staining
CHO
cell
membranes
demonstrates
that
great
candidates
applications,
proposed
strategy
easy
implement
when
structure
known.
Chemical Communications,
Journal Year:
2024,
Volume and Issue:
60(24), P. 3315 - 3318
Published: Jan. 1, 2024
DNA-stabilized
silver
nanoclusters
with
10
to
30
atoms
are
interesting
biocompatible
nanomaterials
intriguing
fluorescence
properties.
However,
they
not
well
understood,
since
atom-scale
high
level
theoretical
calculations
have
been
possible
due
a
lack
of
firm
experimental
structural
information.
Here,
by
using
density
functional
theory
(DFT),
we
study
the
recently
atomically
resolved
(DNA)
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(4), P. 3002 - 3010
Published: Jan. 16, 2024
Silver
cations
can
mediate
base
pairing
of
guanine
(G)
DNA
oligomers,
yielding
linear
parallel
G–Ag+–G
duplexes
with
enhanced
stabilities
compared
to
those
canonical
duplexes.
To
enable
their
use
in
programmable
nanotechnologies,
it
is
critical
understand
solution-state
formation
and
the
nanomechanical
stiffness
Using
temperature-controlled
circular
dichroism
(CD)
spectroscopy,
we
find
that
heating
mixtures
G
oligomers
silver
salt
above
50
°C
fully
destabilizes
G-quadruplex
structures
converts
Electrospray
ionization
mass
spectrometry
supports
form
at
stoichiometries
1
Ag+
per
pair,
CD
spectroscopy
suggests
as
Ag+/base
stoichiometry
increases
further,
undergo
additional
morphological
changes.
liquid-phase
atomic
force
microscopy,
this
excess
enables
assembly
long
fiberlike
∼2.5
nm
heights
equivalent
a
single
duplex
but
lengths
far
exceed
duplex.
Finally,
using
conditions
established
duplexes,
surface
forces
apparatus
(SFA)
compare
solution-phase
dG-dC
Watson–Crick–Franklin
SFA
shows
are
1.3
times
stiffer
than
confirming
gas-phase
ion
mobility
measurements
computational
predictions.
These
findings
may
guide
development
structural
nanotechnologies
rely
on
silver-mediated
pairing.
Nanoscale,
Journal Year:
2024,
Volume and Issue:
16(21), P. 10087 - 10107
Published: Jan. 1, 2024
In
the
dynamic
landscape
of
nanotechnology,
atomically
precise
silver
nanoclusters
(Ag
NCs)
have
emerged
as
a
novel
and
promising
category
materials
with
their
fascinating
properties
enormous
potential.
However,
recent
research
endeavors
surged
towards
stabilizing
Ag-based
NCs,
leading
to
innovative
strategies
like
connecting
cluster
nodes
organic
linkers
construct
hierarchical
structures,
thus
forming
cluster-assembled
(CAMs).
This
approach
not
only
enhances
structural
stability,
but
also
unveils
unprecedented
opportunities
for
CAMs,
overcoming
limitations
individual
Ag
NCs.
this
context,
review
delves
into
captivating
realm
nitrogen-based
ligand
bonded
Ag(I)-based
providing
insights
synthetic
strategies,
structure-property
relationships,
diverse
applications.
We
navigate
challenges
advancements
in
integrating
Ag(I)
nodes,
bound
by
argentophilic
interactions,
highly
connected
periodic
frameworks
different
dimensionalities
using
linkers.
Despite
inherent
diversity
among
CAMs
demonstrate
potential
sensing,
catalysis,
bio-imaging,
device
fabrication,
which
all
are
discussed
review.
Therefore,
gaining
insight
nanocluster
assembly
process
will
offer
valuable
information,
can
enlighten
readers
on
design
advancement
state-of-the-art
The Journal of Physical Chemistry Letters,
Journal Year:
2023,
Volume and Issue:
14(17), P. 4078 - 4083
Published: April 25, 2023
Due
to
desirable
optical
properties,
such
as
efficient
luminescence
and
large
Stokes
shift,
DNA-templated
silver
nanoclusters
(DNA-AgNCs)
have
received
significant
attention
over
the
past
decade.
Nevertheless,
excited-state
dynamics
of
these
systems
are
poorly
understood,
studies
processes
ultimately
leading
a
fluorescent
state
scarce.
Here
we
investigate
early
time
relaxation
16-atom
cluster
(DNA-Ag16NC)
featuring
NIR
emission
in
combination
with
an
unusually
shift
5000
cm–1.
We
follow
photoinduced
DNA-Ag16NC
on
ranges
from
tens
femtoseconds
nanoseconds
using
ultrafast
spectroscopies,
extract
kinetic
model
clarify
physical
picture
dynamics.
expect
obtained
contribute
guiding
research
efforts
toward
elucidating
electronic
structure
novel
objects
their
potential
applications
fluorescence-based
labeling,
imaging,
sensing.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(8), P. 3480 - 3488
Published: Feb. 14, 2024
To
address
the
limitations
of
typical
hairpin-structural
molecular
beacons,
exploring
ability
a
quasi-molecular
beacon
(qMB)
to
create
label-free
fluorescence
biosensors
is
intriguing
and
remains
challenge.
Herein,
we
propose
first
example
modular
qMB
with
feature
stimulation-responsive
conformation
switch
develop
an
aggregated
Ag
nanocluster
(aAgNC)
in
bifurcated
DNA
scaffold
for
fluorescently
sensing
specific
initiator
(I*).
This
was
well
designed
program
four
functional
modules:
I*-recognizable
element
adopting
metastable
stem-loop
bihairpin
structure
two
splits
(exposed
C3GT4
locked
C4AC4T)
aAgNC
template
that
separated
by
tunable
hairpin
spacer
customized
combination
selective
recognition
signaling
readout.
When
presenting
I*
assay
route,
hybridization
induces
directional
disassembly
unit,
on
which
configurationally
switched
liberate
split.
Thus,
parent
pair
C3GT4/C4AC4T
proximal,
affording
situ
nucleation
clustering
emissive
aAgNC.
By
collecting
signal,
quantitative
detection
achieved.
Benefiting
from
ingenious
programming
qMB,
recognizing
integration
actuates
construction
facile
convenient
fluorescent
biosensor
featuring
rapid
reaction
kinetics,
wide
linear
range,
high
sensitivity,
specificity.
would
provide
new
paradigm
exploit
versatile
qMB-based
biosensing
platforms
via
switches
developing
various
DNA-scaffolded
clusters.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 24, 2024
Atomically
precise
metal
nanoclusters
(MNCs)
represent
a
fascinating
class
of
ultrasmall
nanoparticles
with
molecule-like
properties,
bridging
conventional
metal-ligand
complexes
and
nanocrystals.
Despite
their
potential
for
various
applications,
synthesis
challenges
such
as
understanding
varied
synthetic
parameters
property-driven
persist,
hindering
full
exploitation
wider
application.
Incorporating
smart
methodologies,
including
closed-loop
framework
automation,
data
interpretation,
feedback
from
AI,
offers
promising
solutions
to
address
these
challenges.
In
this
perspective,
we
summarize
the
that
has
been
demonstrated
in
nanomaterials
explore
research
frontiers
MNCs.
Moreover,
perspectives
on
inherent
opportunities
MNCs
are
discussed,
aiming
provide
insights
directions
future
advancements
emerging
field
AI
Science,
while
integration
deep
learning
algorithms
stands
substantially
enrich
by
offering
enhanced
predictive
capabilities,
optimization
strategies,
control
mechanisms,
thereby
extending
MNC
synthesis.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(13), P. 12862 - 12874
Published: June 21, 2023
Unraveling
the
transport
of
drugs
and
nanocarriers
in
cerebrovascular
networks
is
important
for
pharmacokinetic
hemodynamic
studies
but
challenging
due
to
complexity
sensing
individual
particles
within
circulatory
system
a
live
animal.
Here,
we
demonstrate
that
DNA-stabilized
silver
nanocluster
(DNA-Ag16NC)
emits
first
near-infrared
window
upon
two-photon
excitation
second
NIR
can
be
used
multiphoton
vivo
fluorescence
correlation
spectroscopy
measurement
cerebral
blood
flow
rates
mice
with
high
spatial
temporal
resolution.
To
ensure
bright
stable
emission
during
experiments,
loaded
DNA-Ag16NCs
into
liposomes,
which
served
dual
purposes
concentrating
fluorescent
label
protecting
it
from
degradation.
DNA-Ag16NC-loaded
liposomes
enabled
quantification
velocities
vessels
living
mouse.
