The
development
of
membrane-bound
protocells,
which
process
cascade
biochemical
reactions
in
distinct
microcompartments,
marks
a
significant
advancement
soft
systems.
However,
many
synthesized
protocells
with
cell
membrane-like
structures
are
prone
to
rupturing
biological
environments
and
challenging
functionalize,
limiting
their
biomedical
applications.
In
this
study,
we
explore
the
liquid-liquid
phase
separation
tannic
acid
(TA)
polyethylene
glycol
(PEG)
form
coacervate
droplets.
Upon
introducing
polyvinylpyrrolidone
(PVP)
molecules,
dense
hydrogen
bonding
network
spontaneously
forms
at
surfaces
droplets,
resulting
robust
fluidic
(FMPs).
These
can
be
flexibly
postfunctionalized
incorporate
functional
nanomaterials
via
electrostatic
attraction,
enabling
design
for
To
demonstrate
this,
nanozymes
(Pt/CeO
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 21, 2024
Novel
biomaterials
are
becoming
more
crucial
in
treating
human
diseases.
However,
many
materials
require
complex
artificial
modifications
and
synthesis,
leading
to
potential
difficulties
preparation,
side
effects,
clinical
translation.
Recently,
significant
progress
has
been
achieved
terms
of
direct
self-assembly
natural
products
from
herbal
medicine
(NPHM),
an
important
source
for
novel
medications,
resulting
a
wide
range
bioactive
supramolecular
including
gels,
nanoparticles.
The
NPHM-based
produced
renewable
resources,
simple
prepare,
have
demonstrated
multi-functionality
slow-release,
smart-responsive
release,
especially
possess
powerful
biological
effects
treat
various
In
this
review,
revealed
as
emerging,
revolutionary,
promising
strategy.
development,
advantages,
limitations
NPHM,
well
the
advantageous
position
materials,
first
reviewed.
Subsequently,
systematic
comprehensive
analysis
strategies
specific
seven
major
classes
NPHM
is
highlighted.
Insights
into
influence
structural
features
on
formation
also
provided.
Finally,
drivers
preparations
summarized,
emphasizing
biomedical
applications,
future
scientific
challenges,
opportunities,
with
hope
igniting
inspiration
research
applications.
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(22), С. 10800 - 10826
Опубликована: Янв. 1, 2024
This
review
provides
a
guideline
for
the
rational
design
of
metal–phenolic
network
(MPN)
composites—which
are
fabricated
from
MPN
and
one
or
more
functional
components
(
e.g.
,
drugs,
proteins)—for
various
applications
across
diverse
disciplines.
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Апрель 9, 2024
Dynamic
microscale
droplets
produced
by
liquid-liquid
phase
separation
(LLPS)
have
emerged
as
appealing
biomaterials
due
to
their
remarkable
features.
However,
the
instability
of
limits
construction
population-level
structures
with
collective
behaviors.
Here
we
first
provide
a
brief
background
in
context
materials
properties.
Subsequently,
discuss
current
strategies
for
stabilizing
including
physical
and
chemical
modulation.
We
also
recent
development
LLPS
various
applications
such
synthetic
cells
biomedical
materials.
Finally,
give
insights
on
how
stabilized
can
self-assemble
into
higher-order
displaying
coordinated
functions
fully
exploit
potentials
bottom-up
biology
applications.
Soft Matter,
Год журнала:
2025,
Номер
21(10), С. 1781 - 1812
Опубликована: Янв. 1, 2025
Peptide-mediated
liquid–liquid
phase
separation
(LLPS)
underpins
the
formation
of
dynamic
biomolecular
condensates,
regulated
by
diverse
molecular
interactions,
and
highlights
potential
applications
in
drug
delivery
synthetic
biology.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(11), С. 13573 - 13584
Опубликована: Март 5, 2024
Periodontitis,
a
complex
inflammatory
disease
initiated
by
bacterial
infections,
presents
significant
challenge
in
public
health.
The
increased
levels
of
reactive
oxygen
species
and
the
subsequent
exaggerated
immune
response
associated
with
periodontitis
often
lead
to
alveolar
bone
resorption
tooth
loss.
Herein,
we
develop
multifunctional
metal–phenolic
composites
(i.e.,
Au@MPN-BMP2)
address
nature
periodontitis,
where
gold
nanoparticles
(AuNPs)
are
coated
networks
(MPNs)
morphogenetic
protein
2
(BMP2).
In
this
design,
MPNs
exhibit
remarkable
antibacterial
antioxidant
properties,
AuNPs
BMP2
promote
osteogenic
differentiation
marrow
mesenchymal
stem
cells
under
conditions.
rat
model
treatment
Au@MPN-BMP2
leads
notable
therapeutic
outcomes,
including
mitigated
oxidative
stress,
reduced
progression
inflammation,
prevention
These
results
highlight
multifunctionality
as
promising
approach
for
addressing
both
microbial
causative
factors
an
overactivated
response.
We
envision
that
rational
design
will
provide
versatile
nanoplatforms
tissue
regeneration
potential
clinical
applications.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 2, 2025
Interfacial
properties
determine
biomaterial
performances,
such
as
cell
adhesion,
signal
exchange,
and
biomineralization,
which
affect
the
tissue
repair
cycle
efficiency
of
clinical
applications.
Peptides,
short
protein
sequences
that
have
defined
functionalities,
are
highly
stable
easy
to
synthesize
enormous
potential
reshape
interfacial
properties.
However,
lack
a
universal
strategy
for
presenting
peptides
on
various
substrates
substantially
hinders
application
peptides.
In
this
study,
we
report
facile
customizing
desired
functionalities
by
well-known
layer-by-layer
(LbL)
technique
through
assembly
polyphenols
with
positively
charged
peptide-coupling
functional
sequences.
Polyphenol–peptide
interactions
were
elucidated
in
detail
assembling
possessing
different
characteristics
(charged,
uncharged,
hydrophobic,
sequence
length)
combination
molecular
dynamics
simulations,
isothermal
titration
calorimetry
further
revealed
favorable
enthalpy
change
due
electrostatic
is
main
driving
force
polyphenols.
LbL
coatings
assembled
from
exhibited
good
substrate
generalization,
stability,
proliferation,
antioxidant
properties,
when
prepared
hollow
capsules
sacrificing
template,
significant
pH
ultrasound
stimulation
responses,
could
be
suitable
candidates
drug
carriers.
Most
importantly,
utilized
present
molecules
(such
arginyl–glycyl–aspartic
acid
(RGD),
adhesion
motif;
CM15,
an
antibacterial
peptide;
PEG,
antifouling
surface)
surfaces.
This
study
not
only
provides
new
insights
into
understanding
regulation
between
proteins/peptides
but
also
paves
way
toward
functionalization
biomaterials.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 24, 2024
Abstract
Stability
issues
in
membrane-free
coacervates
have
been
addressed
with
coating
strategies,
but
these
approaches
often
compromise
the
permeability
of
coacervate.
Here
we
report
a
facile
approach
to
maintain
both
stability
and
using
tannic
acid
then
demonstrate
value
this
enzyme-triggered
drug
release.
First,
develop
size-tunable
via
self-assembly
heparin
glycosaminoglycan
tyrosine
arginine-based
peptides.
A
thrombin-recognition
site
within
peptide
building
block
results
release
upon
thrombin
proteolysis.
Notably,
polyphenols
are
integrated
nano-coacervates
improve
biofluids.
Phenolic
crosslinking
at
liquid-liquid
interface
enables
exceptional
structural
integrity
across
various
environments.
We
discover
pivotal
polyphenol
threshold
for
preserving
enzymatic
activity
alongside
enhanced
stability.
The
disassembly
rate
increases
as
function
activity,
thus
preventing
coagulation
cascade.
This
polyphenol-based
not
only
improves
also
opens
way
applications
biomedicine,
protease
sensing,
bio-responsive
delivery.
Biomacromolecules,
Год журнала:
2024,
Номер
25(9), С. 5468 - 5488
Опубликована: Авг. 23, 2024
Biomolecular
condensates
are
dynamic
liquid
droplets
through
intracellular
liquid-liquid
phase
separation
that
function
as
membraneless
organelles,
which
highly
involved
in
various
complex
cellular
processes
and
functions.
Artificial
analogs
formed
via
similar
pathways
can
be
integrated
with
biological
complexity
advanced
functions
have
received
tremendous
research
interest
the
field
of
synthetic
biology.
The
coacervate
droplet-based
compartments
partition
concentrate
a
wide
range
solutes,
regarded
attractive
candidates
for
mimicking
phase-separation
behaviors
biophysical
features
biomolecular
condensates.
use
peptide-based
materials
phase-separating
components
has
advantages
such
diversity
amino
acid
residues
customized
sequence
design,
allows
programming
their
physicochemical
properties
resulting
compartments.
In
this
Perspective,
we
highlight
recent
advancements
design
construction
biomimicry
from
peptides
relevant
to
protein,
specific
reference
molecular
self-assembly
separation,
biorelated
applications,
envisage
emerging
biomedical
delivery
vehicles.
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 5, 2024
Abstract
Utilizing
enzyme
cascades
as
a
promising
approach
for
targeted
cancer
therapies
holds
significant
potential,
yet
its
clinical
effectiveness
is
substantially
hindered
by
functional
losses
during
delivery.
Complex
coacervation
emerges
an
intriguing
strategy
designing
nanoreactors.
In
this
study,
noteworthy
achievement
presented
in
the
development
of
lactobionic
acid‐modified
tumor
microenvironment
(TME)‐responsive
polyelectrolyte
complex
vesicles
(HGS‐PCVs)
based
on
bioinspired
homopolypeptoids,
which
serve
facile,
intelligent,
and
highly
efficient
nanoreactor
tunable
glucose
oxidase,
hemoglobin,
sorafenib
(SRF)
to
hepatic
cells.
The
TME‐responsive
permeability
HGS‐PCVs
enables
selective
entry
into
their
interior,
triggering
enzymatic
cascade
reaction
within
tumor.
This
intricate
process
generates
toxic
hydroxyl
radicals
while
concurrently
lowering
pH.
Consequently,
pH
shift
enhances
SRF
release,
effectively
promoting
ferroptosis
apoptosis
target
Further,
administration
not
only
initiates
immunogenic
cell
death
but
also
plays
crucial
role
inducing
maturation
dendritic
cells
lymph
nodes.
It
stimulates
adaptive
T‐cell
response,
mechanism
that
contributes
impeding
growth
distant
tumors
vivo,
demonstrating
potential
PCVs
immunotherapy.
