STAR Protocols, Год журнала: 2025, Номер 6(2), С. 103742 - 103742
Опубликована: Апрель 7, 2025
Язык: Английский
Advanced Functional Materials, Год журнала: 2024, Номер 34(32)
Опубликована: Апрель 8, 2024
Abstract The challenges of replicating the complex mechanical and structural diversity natural tissues in vitro by leveraging multi‐material digital light processing (DLP) bioprinting are addressed. This technique utilizes PEGDA‐AAm bio‐ink to develop multi‐component, cell‐laden hydrogel constructs with varying moduli. These not only possess heterogeneous properties but also feature architectures precisely engineered surface microstructures. microfluidic chips successfully fabricated perfusable microchannels, embedding various cell types within matrix. approach enables intricate structures unique topologies, such as spiral grooves triply periodic minimal surfaces (TPMS), which effectively influence alignment, spreading, migration. By integrating PA hydrogels, diverse moduli biological tissues, including bone, liver lobules, vascular networks replicated. ensures high‐fidelity differentiation between regions. findings provide valuable insights into impact substrate modulus structure on behavior, underscoring potential multi‐modulus creating sophisticated custom‐tailored properties. study significantly advances field demonstrating feasibility effectiveness DLP developing complex, functionally relevant tissue models for engineering regenerative medicine.
Язык: Английский
Процитировано
16
Theranostics, Год журнала: 2024, Номер 14(5), С. 2151 - 2166
Опубликована: Янв. 1, 2024
Background:The therapeutic benefits of targeting follicle-stimulating hormone (FSH) receptor in treatment ovarian cancer are significant, whereas the role FSH progresses and underlying mechanism remains to be developed.Methods: Tissue microarray human cancer, tumor xenograft mouse model, vitro cell culture were used investigate carcinogenesis.siRNA, lentivirus inhibitors trigger inactivation genes, plasmids increase transcription genes.Specifically, pathological characteristic was assessed by histology immunohistochemistry (IHC), while signaling pathway studied using western blot, quantitative RT-PCR, immunofluorescence.Results: Histology IHC normal tissue confirmed association between Snail metastasis.Moreover, epithelial cells mice, showed promote mesenchymal transition (EMT) progress metastasis via prolonging half-life mRNA a N6-methyladenine methylation (m6A) dependent manner, which mechanistically through CREB/ALKBH5 pathway.Conclusions: These findings indicated that induces EMT progression CREB/ALKBH5/Snail pathway.Thus, this study provided new insight into strategy patients with high level FSH.
Язык: Английский
Процитировано
9
Biomolecules, Год журнала: 2024, Номер 14(5), С. 585 - 585
Опубликована: Май 15, 2024
Epithelial ovarian cancer (EOC) is one of the most aggressive forms gynaecological malignancies. Survival rates for women diagnosed with OC remain poor as patients are advanced disease. Debulking surgery and platinum-based therapies current mainstay treatment. However, despite achieving initial remission, a significant portion will relapse because innate acquired resistance, at which point disease considered incurable. In view this, novel detection strategies therapeutic approaches needed to improve outcomes survival patients. this review, we summarize our knowledge genetic landscape molecular pathways underpinning its many subtypes. By examining explored in preclinical clinical settings, highlight importance decoding how single convergent alterations co-exist drive progression resistance treatments. We also propose that core signalling such PI3K MAPK play critical roles origin diverse subtypes can become new targets combination known DNA damage repair development tailored more effective anti-cancer
Язык: Английский
Процитировано
9
Cancer Letters, Год журнала: 2025, Номер 612, С. 217410 - 217410
Опубликована: Янв. 16, 2025
Язык: Английский
Процитировано
1
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 30, 2025
Abstract Cancer cells cope with oxidative stress for their proliferation and metastasis by equipping antioxidant systems, among which the enzymes peroxiredoxins (PRDXs) play crucial roles. However, whether PRDXs exhibit nonenzymatic functions remains unclear. Here, it is shown that 1‐cysteine PRDX (PRDX6) upregulates nicotinamide N ‐methyltransferase (NNMT) to promote growth of ovarian cancer cells, independently PRDX6's enzymatic activities. Mechanistically, PRDX6 interacts NNMT prevent its binding E3 ubiquitin ligase tripartite‐motif protein 56 (TRIM56), leading inhibition ubiquitination at lysine 23 210 suppression subsequent proteasomal degradation. In addition, PRDX6‐mediated upregulation activates mitogen‐activated kinase (MAPK) signaling, thereby promoting cells. Notably, overexpression associated higher levels in human tissues predictive poor prognosis patients. Overall, findings illustrate a critical oncogenic mechanism enzyme progression beyond mechanisms.
Язык: Английский
Процитировано
1
Drug Delivery and Translational Research, Год журнала: 2025, Номер unknown
Опубликована: Янв. 31, 2025
Язык: Английский
Процитировано
1
Biomedicines, Год журнала: 2024, Номер 12(3), С. 502 - 502
Опубликована: Фев. 23, 2024
The fact that the Mediterranean diet could represent a source of natural compounds with cancer-preventive and therapeutic activity has been object great interest, especially regard to mechanisms action polyphenols found in olive oil leaves. Secoiridoid oleuropein (OLE) its derivative hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) have demonstrated anti-proliferative properties against variety tumors hematological malignancies both vivo vitro, measurable effects on cellular redox status, metabolism, transcriptional activity. With this review, we aim summarize most up-to-date information potential use OLE HT for cancer treatment, making important considerations about bioavailability, OLE- HT-mediated drug dual as pro- antioxidants, likely hampering their clinical routine. Also, focus details available nutritionally relevant concentrations cell viability, homeostasis, inflammation order evaluate if be considered agents or new chemotherapy drugs whenever only is represented by diet.
Язык: Английский
Процитировано
6
MedComm, Год журнала: 2024, Номер 5(9)
Опубликована: Авг. 28, 2024
Ovarian cancer is high recurrence and mortality malignant tumor. The most common ovarian was High-Grade Serous Cancer. However, Cancer organoid rare, which with patient immune microenvironment blood vessels even absence. Here, we report a novel system derived from samples. These organoids recapitulate organoids' histological molecular heterogeneity while preserving the critical vessels, as evidenced by presence of
Язык: Английский
Процитировано
5
EBioMedicine, Год журнала: 2025, Номер 114, С. 105646 - 105646
Опубликована: Март 14, 2025
Язык: Английский
Процитировано
0
Frontiers in Oncology, Год журнала: 2024, Номер 14
Опубликована: Май 2, 2024
Purpose This study aims to systematically compare the diagnostic performance of Ovarian-Adnexal Reporting and Data System with International Ovarian Tumor Analysis Simple Rules Assessment Different NEoplasias in adneXa model for risk stratification ovarian cancer adnexal masses. Methods A literature search online databases relevant studies up July 2023 was conducted by two independent reviewers. The summary estimates were pooled hierarchical receiver-operating characteristic model. quality included assessed Quality Diagnostic Accuracy Studies–2 Studies-Comparative Tool. Metaregression subgroup analyses performed explore impact varying clinical settings. Results total 13 met inclusion criteria. sensitivity specificity eight head-to-head between 0.96 (95% CI 0.92–0.98) 0.82 0.71–0.90) vs. 0.94 0.91–0.95) 0.83 0.77–0.88), respectively, seven Rules, 0.95 0.93–0.97) 0.75 0.62–0.85) 0.91 0.82–0.96) 0.86 0.76–0.93), respectively. No significant differences found as well terms ( P = 0.57 0.21) 0.87 0.12). Substantial heterogeneity observed among all three guidelines. Conclusion All guidelines demonstrated high performance, no or
Язык: Английский
Процитировано
4