Cited by Protocol to profile tumor and microenvironment from ovarian cancer patient samples and evaluate cell-based therapy using in vitro killing assays

Multi‐Material Digital Light Processing (DLP) Bioprinting of Heterogeneous Hydrogel Constructs with Perfusable Networks DOI

Ming Yang,

Lang Chu,

Yanfeng Zhuang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(32)

Published: April 8, 2024

Abstract The challenges of replicating the complex mechanical and structural diversity natural tissues in vitro by leveraging multi‐material digital light processing (DLP) bioprinting are addressed. This technique utilizes PEGDA‐AAm bio‐ink to develop multi‐component, cell‐laden hydrogel constructs with varying moduli. These not only possess heterogeneous properties but also feature architectures precisely engineered surface microstructures. microfluidic chips successfully fabricated perfusable microchannels, embedding various cell types within matrix. approach enables intricate structures unique topologies, such as spiral grooves triply periodic minimal surfaces (TPMS), which effectively influence alignment, spreading, migration. By integrating PA hydrogels, diverse moduli biological tissues, including bone, liver lobules, vascular networks replicated. ensures high‐fidelity differentiation between regions. findings provide valuable insights into impact substrate modulus structure on behavior, underscoring potential multi‐modulus creating sophisticated custom‐tailored properties. study significantly advances field demonstrating feasibility effectiveness DLP developing complex, functionally relevant tissue models for engineering regenerative medicine.

Language: Английский

Citations

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation DOI

Xingyan Xu, Xuefen Zhuang,

Haowei Yu

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2151 - 2166

Published: Jan. 1, 2024

Background:The therapeutic benefits of targeting follicle-stimulating hormone (FSH) receptor in treatment ovarian cancer are significant, whereas the role FSH progresses and underlying mechanism remains to be developed.Methods: Tissue microarray human cancer, tumor xenograft mouse model, vitro cell culture were used investigate carcinogenesis.siRNA, lentivirus inhibitors trigger inactivation genes, plasmids increase transcription genes.Specifically, pathological characteristic was assessed by histology immunohistochemistry (IHC), while signaling pathway studied using western blot, quantitative RT-PCR, immunofluorescence.Results: Histology IHC normal tissue confirmed association between Snail metastasis.Moreover, epithelial cells mice, showed promote mesenchymal transition (EMT) progress metastasis via prolonging half-life mRNA a N6-methyladenine methylation (m6A) dependent manner, which mechanistically through CREB/ALKBH5 pathway.Conclusions: These findings indicated that induces EMT progression CREB/ALKBH5/Snail pathway.Thus, this study provided new insight into strategy patients with high level FSH.

Language: Английский

Citations

Oncogenic Pathways and Targeted Therapies in Ovarian Cancer DOI

Carolina Lliberos,

Gary Richardson, Antonella Papa

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(5), P. 585 - 585

Published: May 15, 2024

Epithelial ovarian cancer (EOC) is one of the most aggressive forms gynaecological malignancies. Survival rates for women diagnosed with OC remain poor as patients are advanced disease. Debulking surgery and platinum-based therapies current mainstay treatment. However, despite achieving initial remission, a significant portion will relapse because innate acquired resistance, at which point disease considered incurable. In view this, novel detection strategies therapeutic approaches needed to improve outcomes survival patients. this review, we summarize our knowledge genetic landscape molecular pathways underpinning its many subtypes. By examining explored in preclinical clinical settings, highlight importance decoding how single convergent alterations co-exist drive progression resistance treatments. We also propose that core signalling such PI3K MAPK play critical roles origin diverse subtypes can become new targets combination known DNA damage repair development tailored more effective anti-cancer

Language: Английский

Citations

STING in Cancer Immunoediting: Modeling Tumor-Immune Dynamics Throughout Cancer Development DOI

Xiao Zhang, Yan Chen,

Xi Liu

et al.

Cancer Letters, Journal Year: 2025, Volume and Issue: 612, P. 217410 - 217410

Published: Jan. 16, 2025

Language: Английский

Citations

PRDX6 Prevents NNMT Ubiquitination and Degradation as a Nonenzymatic Mechanism to Promote Ovarian Cancer Progression DOI

Xingyun Wu,

Li Luo, Mao Wang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Abstract Cancer cells cope with oxidative stress for their proliferation and metastasis by equipping antioxidant systems, among which the enzymes peroxiredoxins (PRDXs) play crucial roles. However, whether PRDXs exhibit nonenzymatic functions remains unclear. Here, it is shown that 1‐cysteine PRDX (PRDX6) upregulates nicotinamide N ‐methyltransferase (NNMT) to promote growth of ovarian cancer cells, independently PRDX6's enzymatic activities. Mechanistically, PRDX6 interacts NNMT prevent its binding E3 ubiquitin ligase tripartite‐motif protein 56 (TRIM56), leading inhibition ubiquitination at lysine 23 210 suppression subsequent proteasomal degradation. In addition, PRDX6‐mediated upregulation activates mitogen‐activated kinase (MAPK) signaling, thereby promoting cells. Notably, overexpression associated higher levels in human tissues predictive poor prognosis patients. Overall, findings illustrate a critical oncogenic mechanism enzyme progression beyond mechanisms.

Language: Английский

Citations

Innovative landscapes in intraperitoneal therapy of ovarian cancer DOI

K. Kumar,

M. S. Madhusoodanan,

Meghna Pangath

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

Use of Oleuropein and Hydroxytyrosol for Cancer Prevention and Treatment: Considerations about How Bioavailability and Metabolism Impact Their Adoption in Clinical Routine DOI

Francesco Gervasi, Fanny Pojero

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 502 - 502

Published: Feb. 23, 2024

The fact that the Mediterranean diet could represent a source of natural compounds with cancer-preventive and therapeutic activity has been object great interest, especially regard to mechanisms action polyphenols found in olive oil leaves. Secoiridoid oleuropein (OLE) its derivative hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) have demonstrated anti-proliferative properties against variety tumors hematological malignancies both vivo vitro, measurable effects on cellular redox status, metabolism, transcriptional activity. With this review, we aim summarize most up-to-date information potential use OLE HT for cancer treatment, making important considerations about bioavailability, OLE- HT-mediated drug dual as pro- antioxidants, likely hampering their clinical routine. Also, focus details available nutritionally relevant concentrations cell viability, homeostasis, inflammation order evaluate if be considered agents or new chemotherapy drugs whenever only is represented by diet.

Language: Английский

Citations

Patient‐derived ovarian cancer organoid carries immune microenvironment and blood vessel keeping high response to cisplatin DOI

Yuqing Zhao, Chen Wang, Wei Deng

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(9)

Published: Aug. 28, 2024

Ovarian cancer is high recurrence and mortality malignant tumor. The most common ovarian was High-Grade Serous Cancer. However, Cancer organoid rare, which with patient immune microenvironment blood vessels even absence. Here, we report a novel system derived from samples. These organoids recapitulate organoids' histological molecular heterogeneity while preserving the critical vessels, as evidenced by presence of

Language: Английский

Citations

VIPAS39 confers ferroptosis resistance in epithelial ovarian cancer through exporting ACSL4 DOI

Yuening Jiang,

Jie Li, Tianzhen Wang

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105646 - 105646

Published: March 14, 2025

Language: Английский

Citations

Comparison of O-RADS with the ADNEX model and IOTA SR for risk stratification of adnexal lesions: a systematic review and meta-analysis DOI

Jing Han,

Jing Wen, Wei Hu

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: May 2, 2024

Purpose This study aims to systematically compare the diagnostic performance of Ovarian-Adnexal Reporting and Data System with International Ovarian Tumor Analysis Simple Rules Assessment Different NEoplasias in adneXa model for risk stratification ovarian cancer adnexal masses. Methods A literature search online databases relevant studies up July 2023 was conducted by two independent reviewers. The summary estimates were pooled hierarchical receiver-operating characteristic model. quality included assessed Quality Diagnostic Accuracy Studies–2 Studies-Comparative Tool. Metaregression subgroup analyses performed explore impact varying clinical settings. Results total 13 met inclusion criteria. sensitivity specificity eight head-to-head between 0.96 (95% CI 0.92–0.98) 0.82 0.71–0.90) vs. 0.94 0.91–0.95) 0.83 0.77–0.88), respectively, seven Rules, 0.95 0.93–0.97) 0.75 0.62–0.85) 0.91 0.82–0.96) 0.86 0.76–0.93), respectively. No significant differences found as well terms ( P = 0.57 0.21) 0.87 0.12). Substantial heterogeneity observed among all three guidelines. Conclusion All guidelines demonstrated high performance, no or

Language: Английский

Citations