Nanocarriers based on bacterial membrane materials for cancer vaccine delivery

Nature Protocols, Год журнала: 2022, Номер 17(10), С. 2240 - 2274

Опубликована: Июль 25, 2022

Here we present a protocol for the construction and use of two types nanocarrier based on bacterial membrane materials cancer vaccine delivery. Cancer vaccines induce tumor regression through triggering specific T-cell responses against neoantigens, process that can be enhanced by Inspired body's natural immune defenses invasion, have developed different materials, which employ genetically engineered outer-membrane vesicles (OMVs), or hybrid containing cytoplasmic membrane, respectively. The OMV-based nanocarriers rapidly display antigens surface modified Plug-and-Display system, suitable customized when neoantigens identified. membrane-based are prepared fusion primary cell from surgically removed tissues, possessing unique advantages as personalized not readily available. Compared with chemically synthesized such liposomes polymer without intrinsic adjuvant properties, owing to large amounts pathogen-associated molecular patterns, activate antigen-presenting cells while delivering multiple antigens, thus inducing effective antigen presentation robust adaptive activation. Excluding culture tissue collection, preparation OMV- takes ~8 h 10 construction, We also detail how these create nanovaccines evaluate their immunostimulatory antitumor effects. This details in delivery nanovaccines.

Язык: Английский

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Nanoparticle-based medicines in clinical cancer therapy

Nano Today, Год журнала: 2022, Номер 45, С. 101512 - 101512

Опубликована: Июнь 4, 2022

Язык: Английский

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Stimuli-Responsive Polymer-Based Nanosystems for Cancer Theranostics

ACS Nano, Год журнала: 2023, Номер 17(23), С. 23223 - 23261

Опубликована: Дек. 2, 2023

Stimuli-responsive polymers can respond to internal stimuli, such as reactive oxygen species (ROS), glutathione (GSH), and pH, biological enzymes, external lasers ultrasound, etc., by changing their hydrophobicity/hydrophilicity, degradability, ionizability, thus have been widely used in biomedical applications. Due the characteristics of tumor microenvironment (TME), stimuli-responsive that cater specifically TME extensively prepare smart nanovehicles for targeted delivery therapeutic diagnostic agents tissues. Compared conventional drug nanosystems, TME-responsive nanosystems many advantages, high sensitivity, broad applicability among different tumors, functional versatility, improved biosafety. In recent years, a great deal research has devoted engineering efficient polymeric significant improvement made both cancer diagnosis therapy. this review, we summarize some advances involving use polymer nanocarriers delivery, imaging, therapy, theranostics. Various chemical stimuli will be described context nanosystems. Accordingly, groups responsible responsiveness strategies incorporate these into discussed detail. With on topic expending at fast pace, innovative concepts, sequential cascade release, NIR-II multifunctional formulations, emerged popular enhanced performance, which also included here with up-to-date illustrations. We hope review offer valuable insights selection optimization help accelerate future applications treatment.

Язык: Английский

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Antigen Capture and Immune Modulation by Bacterial Outer Membrane Vesicles as In Situ Vaccine for Cancer Immunotherapy Post‐Photothermal Therapy

Small, Год журнала: 2022, Номер 18(14)

Опубликована: Фев. 12, 2022

Abstract Tumor antigens released from tumor cells after local photothermal therapy (PTT) can activate the tumor‐specific immune responses, which are critical for eliminating residual lesions and distant metastases. However, limited recognition efficiency of by system immunosuppressive microenvironment lead to ineffective antitumor immunity. Here, an in situ multifunctional vaccine based on bacterial outer membrane vesicles (OMVs, 1‐MT@OMV‐Mal) is developed surface conjunction maleimide groups (Mal) interior loading with inhibitor indoleamine 2, 3‐dioxygenase (IDO), 1‐methyl‐tryptophan (1‐MT). 1‐MT@OMV‐Mal bind PTT, be efficiently recognized taken up dendritic cells. Furthermore, injection simultaneously overcomes inhibition IDO tumor‐infiltrating effector T cells, leading remarkable both primary tumors. Together, a promising OMVs facilitate immune‐mediated clearance PTT through orchestrating antigen capture modulation presented.

Язык: Английский

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Recent Progress of Supramolecular Chemotherapy Based on Host–Guest Interactions

Advanced Materials, Год журнала: 2023, Номер 36(21)

Опубликована: Июль 21, 2023

Abstract Chemotherapy is widely recognized as an effective approach for treating cancer due to its ability eliminate cells using chemotherapeutic drugs. However, traditional chemotherapy suffers from various drawbacks, including limited solubility and stability of drugs, severe side effects, low bioavailability, drug resistance, challenges in tracking treatment efficacy. These limitations greatly hinder widespread clinical application. In contrast, supramolecular chemotherapy, which relies on host–guest interactions, presents a promising alternative by offering highly efficient minimally toxic anticancer delivery. this review, overview recent advancements based interactions provided. The significant role it plays guiding therapy emphasized. Drawing wealth cutting‐edge research, herein, timely valuable resource individuals interested the field or therapy, presented. Furthermore, review contributes progression toward

Язык: Английский

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Engineering Poly(ethylene glycol) Nanoparticles for Accelerated Blood Clearance Inhibition and Targeted Drug Delivery

Journal of the American Chemical Society, Год журнала: 2022, Номер 144(40), С. 18419 - 18428

Опубликована: Сен. 27, 2022

Surface modification with poly(ethylene glycol) (PEGylation) is an effective strategy to improve the colloidal stability of nanoparticles (NPs) and often used minimize cellular uptake clearance NPs by immune system. However, PEGylation can also trigger accelerated blood (ABC) phenomenon, which known reduce circulation time PEGylated NPs. Herein, we report engineering stealth PEG that avoid ABC phenomenon and, when modified hyaluronic acid (HA), show specific cancer cell targeting drug delivery. cross-linked disulfide bonds are prepared using zeolitic imidazolate framework-8 as templates. The reported templating enables simultaneous removal template formation under mild conditions (pH 5.5 buffer). Compared liposomes, secretion anti-PEG antibodies presence IgM IgG did not significantly accelerate NPs, indicating inhibition effect for Functionalization HA affords retain their properties against macrophages, target CD44-expressed cells loaded anticancer doxorubicin, effectively inhibit tumor growth. innovation this study lies in circumvent be functionalized improved targeted delivery drugs.

Язык: Английский

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Biomimetic and bioinspired nano‐platforms for cancer vaccine development

Exploration, Год журнала: 2023, Номер 3(3)

Опубликована: Апрель 25, 2023

The advent of immunotherapy has revolutionized the treating modalities cancer. Cancer vaccine, aiming to harness host immune system induce a tumor-specific killing effect, holds great promises for its broad patient coverage, high safety, and combination potentials. Despite promising, clinical translation cancer vaccines faces obstacles including lack potency, limited options tumor antigens adjuvants, immunosuppressive microenvironment. Biomimetic bioinspired nanotechnology provides new impetus designing concepts vaccines. Through mimicking stealth coating, pathogen recognition pattern, tissue tropism pathogen, other irreplaceable properties from nature, biomimetic could gain functions such as longstanding, targeting, self-adjuvanting, on-demand cargo release. specific behavior endogenous molecules each type living entity (cell or microorganism) offer unique features address needs immunotherapy. In this review, strategies inspired by eukaryotic cells, bacteria, viruses will be overviewed advancing vaccine development. Our insights into future development shared at end expediting translation.

Язык: Английский

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Inhalable Biomineralized Liposomes for Cyclic Ca²⁺-Burst-Centered Endoplasmic Reticulum Stress Enhanced Lung Cancer Ferroptosis Therapy

ACS Nano, Год журнала: 2023, Номер 17(6), С. 5486 - 5502

Опубликована: Март 8, 2023

Lung cancer with the highest mortality poses a great threat to human health. Ferroptosis therapy has recently been raised as promising strategy for lung treatment by boosting reactive species (ROS) production and lipid peroxidation (LPO) accumulation intracellularly. However, insufficient intracellular ROS level unsatisfactory drug in lesions hamper efficacy of ferroptosis therapy. Here, an inhalable biomineralized liposome LDM co-loaded dihydroartemisinin (DHA) pH-responsive calcium phosphate (CaP) was constructed nanoinducer achieving Ca2+-burst-centered endoplasmic reticulum (ER) stress enhanced Equipped excellent nebulization properties, about 6.80-fold higher than intravenous injection made proposed ideal nanoplatform treatment. The Fenton-like reaction mediated DHA peroxide bridge structure could contribute induce ferroptosis. Assisted DHA-mediated sarco-/endoplasmic ATPase (SERCA) inhibition, initial Ca2+ burst caused CaP shell degradation triggered Ca2+-mediated intense ER subsequently induced mitochondria dysfunction further boost accumulation, which strengthens second occurred result influx through ferroptotic pores on cell membranes, thus sequentially constructing lethal "Ca2+ burst-ER stress-ferroptosis" cycle. Consequently, process confirmed swelling membrane disruption driven notable LPO accumulation. showed encouraging retention property extraordinary antitumor ability orthotropic tumor murine model. In conclusion, be potential tailored nebulization-based pulmonary delivery underscore application

Язык: Английский

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One-dimensional nanomaterials for cancer therapy and diagnosis

Chemical Society Reviews, Год журнала: 2023, Номер 52(13), С. 4488 - 4514

Опубликована: Янв. 1, 2023

One-dimensional (1-D) nanomaterials possess unique shape-dependent phyicochemical properties and are increasingly recognized as promising materials for nanotechnology. 1-D can be classified according to their shape, such nanorods, nanotubes, nanowires, self-assembled nanochains, etc., have been applied in electronics, photonics, catalysis. The biological characteristics of nanomaterials, including high drug loading efficiency, prolonged blood circulation, the ability capture cancer cells, cellular uptake mechanisms, efficient photothermal conversion, material tunability, aided extending potential biomedical applications, particularly therapy diagnosis. This review highlights a novel perspective on emerging diagnosis by introducing definition physicochemical properties, recent advances also proposes unexplored nanomaterial types therapeutic applications nanomaterials. In particular, most significant exciting years, ultrasound-enabled sonodynamic therapy, magnetic field-based bioresponsive intracellular self-assembly situ, discussed along with concepts, piezoelectric nanozyme-based nanomedicine, others.

Язык: Английский

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Cancer chemotherapy resistance: Mechanisms and recent breakthrough in targeted drug delivery

European Journal of Pharmacology, Год журнала: 2023, Номер 958, С. 176013 - 176013

Опубликована: Авг. 24, 2023

Язык: Английский

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