Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 4, 2025
Abstract
The
global
population’s
aging
and
growth
will
likely
result
in
an
increase
chronic
aging-related
diseases.
Early
diagnosis
could
improve
the
medical
care
quality
of
life.
Many
diseases
are
linked
to
misfolding
or
conformational
changes
biomarker
peptides
proteins,
which
affect
their
function
binding
properties.
Current
clinical
methods
struggle
detect
quantify
these
changes.
Therefore,
there
is
a
need
for
sensitive
sensors
that
can
low-concentration
analytes
biofluids.
Nanopore
electrical
detection
has
shown
potential
sensing
subtle
protein
peptide
conformation
This
technique
single
molecules
label-free
while
distinguishing
shape
physicochemical
property
Its
proven
sensitivity
makes
nanopore
technology
promising
ultra-sensitive,
personalized
point-of-care
devices.
We
focus
on
capability
detecting
quantifying
modifications
enantiomers
proteins
discuss
this
as
solution
future
societal
health
challenges.
Nature Methods,
Год журнала:
2024,
Номер
21(4), С. 609 - 618
Опубликована: Март 5, 2024
Abstract
Precise
identification
and
quantification
of
amino
acids
is
crucial
for
many
biological
applications.
Here
we
report
a
copper(II)-functionalized
Mycobacterium
smegmatis
porin
A
(MspA)
nanopore
with
the
N91H
substitution,
which
enables
direct
all
20
proteinogenic
when
combined
machine-learning
algorithm.
The
validation
accuracy
reaches
99.1%,
30.9%
signal
recovery.
feasibility
ultrasensitive
was
also
demonstrated
at
nanomolar
range.
Furthermore,
capability
this
system
real-time
analyses
two
representative
post-translational
modifications
(PTMs),
one
unnatural
acid
ten
synthetic
peptides
using
exopeptidases,
including
clinically
relevant
associated
Alzheimer’s
disease
cancer
neoantigens,
demonstrated.
Notably,
our
strategy
successfully
distinguishes
only
difference
from
hydrolysate
provides
possibility
to
infer
peptide
sequence.
Science,
Год журнала:
2024,
Номер
385(6706), С. 282 - 288
Опубликована: Июль 18, 2024
Transmembrane
β-barrels
have
considerable
potential
for
a
broad
range
of
sensing
applications.
Current
engineering
approaches
nanopore
sensors
are
limited
to
naturally
occurring
channels,
which
provide
suboptimal
starting
points.
By
contrast,
de
novo
protein
design
can
in
principle
create
an
unlimited
number
new
nanopores
with
any
desired
properties.
Here
we
describe
general
approach
designing
transmembrane
β-barrel
pores
different
diameters
and
pore
geometries.
Nuclear
magnetic
resonance
crystallographic
characterization
show
that
the
designs
stably
folded
structures
resembling
those
models.
The
distinct
conductances
correlate
their
diameter,
ranging
from
110
picosiemens
(~0.5
nanometer
diameter)
430
(~1.1
diameter).
Our
opens
door
custom
sequencing
Advanced Materials,
Год журнала:
2024,
Номер
36(23)
Опубликована: Март 8, 2024
Abstract
The
sheet‐like
lipid
bilayer
is
the
fundamental
structural
component
of
all
cell
membranes.
Its
building
blocks
are
phospholipids
and
cholesterol.
Their
amphiphilic
structure
spontaneously
leads
to
formation
a
in
aqueous
environment.
Lipids
not
just
elements.
Individual
species,
membrane
structure,
dynamics
influence
regulate
protein
function.
An
exciting
field
emerging
where
membrane‐associated
material
properties
different
systems
used
designing
innovative
solutions
for
widespread
applications
across
various
fields,
such
as
food
industry,
cosmetics,
nano‐
biomedicine,
drug
storage
delivery,
biotechnology,
biosensors,
computing.
Here,
authors
summarize
what
known
about
how
lipids
determine
functions
biological
membranes
this
has
been
or
can
be
translated
into
applications.
Based
on
recent
progress
understanding
dynamics,
physical
properties,
perspective
provided
membrane‐controlled
regulation
extend
current
even
offer
new
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Abstract
Biological
nanopores
are
powerful
tools
for
single‐molecule
detection,
with
promising
potential
as
next‐generation
biosensors.
A
major
bottleneck
in
nanopore
analysis
is
the
fragility
of
supporting
lipid
membranes,
that
easily
rupture
after
exposure
to
biological
samples.
Membranes
comprising
PMOXA‐PDMS‐PMOXA
(poly(2‐methyloxazoline‐b‐dimethylsiloxane‐b‐2‐methyloxazoline))
or
PBD‐PEO
(poly(1,2‐butadiene)‐b‐poly(ethylene
oxide))
polymers
may
form
robust
alternatives,
but
their
suitability
reconstitution
a
broad
range
has
not
yet
been
investigated.
Here,
membranes
found
be
highly
toward
applied
voltages
and
human
serum,
while
providing
poor
environment
reconstitution.
However,
hybrid
containing
similar
molar
ratio
PBD
11
PEO
8
diphytanoyl
phosphatidylcholine
(DPhPC)
lipids
show
best
both
worlds:
suitable
wide
variety
nanopores.
Molecular
dynamics
simulations
reveal
≈12
nm
domains
interspersed
by
polymer
matrix.
Nanopores
partition
into
these
nanodomains
sequester
lipids,
possibly
offering
same
binding
strength
native
bilayer.
reconstituted
yield
efficient
sampling
biomolecules
enable
sensing
high
concentrations
serum.
This
work
thus
shows
functionalized
allow
sensing,
forming
interfaces,
resolving
an
important
novel
nanopore‐based
ACS Nano,
Год журнала:
2024,
Номер
18(27), С. 17521 - 17533
Опубликована: Июнь 4, 2024
Selective
transport
of
ions
through
nanometer-sized
pores
is
fundamental
to
cell
biology
and
central
many
technological
processes
such
as
water
desalination
electrical
energy
storage.
Conventional
methods
for
generating
ion
selectivity
include
placement
fixed
charges
at
the
inner
surface
a
nanopore
either
point
mutations
in
protein
pore
or
chemical
treatment
solid-state
surface,
with
each
type
requiring
custom
approach.
Here,
we
describe
general
method
transforming
nanoscale
into
highly
selective,
anion-conducting
channel
capable
giant
electro-osmotic
effect.
Our
molecular
dynamics
simulations
reverse
potential
measurements
show
that
exposure
biological
high
concentrations
guanidinium
chloride
renders
positively
charged
due
transient
binding
cations
surface.
A
comparison
four
nanopores
reveals
relationship
between
selectivity,
shape,
composition
flow.
Guanidinium
are
also
found
produce
anion
flow
via
same
mechanism.
sticky-ion
approach
generate
can
have
numerous
applications
controlling
detection,
identification,
sequencing
individual
proteins.
Sensors and Actuators Reports,
Год журнала:
2024,
Номер
8, С. 100225 - 100225
Опубликована: Июль 17, 2024
Nanopores
play
essential
roles
in
biological
processes,
such
as
ion
channels
and
pumps
cellular
membranes,
technological
applications
DNA
sequencing.
Advancements
nanofabrication
techniques
have
enabled
the
routine
integration
of
nanopores
into
solid-state
devices,
resulting
a
plethora
analytical
applications.
This
review
explores
recent
developments
nanopore-enabled
electrochemical
systems,
which
transcended
traditional
resistive
pulse
sensing
to
offer
novel
capabilities
single-entity
studies,
stimulus-responsive
gating,
point-of-care
diagnostics.
We
highlight
studies
on
design
utility
nanopore
electrode
arrays,
serve
nanocontainers
capable
isolating
analyzing
single
entities,
extend
discussion
hierarchically
organized,
systems
that
regulate
species
transport
across
nanopores,
enriching
analytes
for
ultrasensitive
detection.
In
addition,
we
utilization
probe-assisted
sensing,
demonstrating
its
efficacy
selectively
binding
detecting
target
molecules
ions.
Finally,
outline
future
directions
nanopore-based
enhance
robustness,
achieve
high-throughput
analysis,
incorporate
artificial
intelligence
materials
data
promising
transformative
impacts
diagnostics
research.
ACS Nano,
Год журнала:
2024,
Номер
18(12), С. 9137 - 9149
Опубликована: Март 12, 2024
Point-of-care
monitoring
of
small
molecules
in
biofluids
is
crucial
for
clinical
diagnosis
and
treatment.
However,
the
inherent
low
degree
recognition
complex
composition
present
significant
obstacles
current
detection
technologies.
Although
nanopore
sensing
excels
analysis
molecules,
direct
remains
a
challenge.
In
this
study,
we
method
molecule
drug
gentamicin
whole
blood
based
on
mechanosensitive
channel
conductance
Pseudomonas
aeruginosa
(PaMscS)
nanopore.
PaMscS
can
directly
detect
distinguish
its
main
components
with
only
monomethyl
difference.
The
'molecular
sieve'
structure
enables
measurement
human
within
10
min.
Furthermore,
continuous
device
constructed
achieved
live
rats
approximately
2.5
h
without
consumption,
while
be
analyzed
situ.
This
approach
rapid
convenient
single-molecule
level
resolution,
which
significantly
lower
threshold
concentration
promote
more
efficient
use.
Moreover,
work
also
lays
foundation
future
development
technology
resolution
living
body.
