Membranes,
Год журнала:
2025,
Номер
15(1), С. 9 - 9
Опубликована: Янв. 1, 2025
The
endoplasmic
reticulum
and
the
internal
nuclear
compartments
are
intrinsically
connected
through
membrane,
pores
lamina.
High
resolution
imaging
of
each
these
cellular
features
concurrently
remains
a
significant
challenge.
To
that
end
we
have
developed
new
molecular
membrane-endoplasmic
(NM-ER)
staining
fluorophore
with
emission
maxima
at
650
nm.
NM-ER
is
compatible
fixed
live
cell
stimulated
depletion
microscopy
(STED)
showing
improvement
in
when
compared
to
comparable
confocal
laser
scanning
microscopy.
versatility
was
illustrated
its
use
other
fluorophores
for
co-imaging
DNA,
lamina
allowing
abnormalities
be
identified.
alone,
or
region
labels
could
an
important
tool
investigation
transport
associated
processes.
Abstract
Despite
the
advent
of
various
medical
interventions
for
cancer
treatment,
disease
continues
to
pose
a
formidable
global
health
challenge,
necessitating
development
new
therapeutic
approaches
more
effective
treatment
outcomes.
Photodynamic
therapy
(PDT),
which
utilizes
light
activate
photosensitizer
produce
cytotoxic
reactive
oxygen
species
(ROS)
eradicating
cells,
has
emerged
as
promising
approach
due
its
high
spatiotemporal
precision
and
minimal
invasiveness.
However,
widespread
clinical
use
PDT
faces
several
challenges,
including
inefficient
production
ROS
in
hypoxic
tumor
microenvironment,
limited
penetration
depth
biological
tissues,
inadequate
accumulation
photosensitizers
at
site.
Over
past
decade,
there
been
increasing
interest
utilization
photofunctional
transition
metal
complexes
applications
their
intriguing
photophysical
photochemical
properties.
This
review
provides
an
overview
current
design
strategies
used
innovative
phototherapeutics,
aiming
address
limitations
associated
with
achieve
The
challenges
future
perspectives
on
translation
are
also
discussed.
National Science Review,
Год журнала:
2023,
Номер
10(11)
Опубликована: Сен. 4, 2023
Abstract
Exploration
of
artificial
aggregation-induced
emission
luminogens
(AIEgens)
has
garnered
extensive
interest
in
the
past
two
decades.
In
particular,
AIEgens
possessing
natural
characteristics
(BioAIEgens)
have
received
more
attention
recently
due
to
advantages
biocompatibility,
sustainability
and
renewability.
However,
extremely
limited
number
BioAIEgens
extracted
from
sources
retarded
their
development.
Herein,
a
new
class
based
on
scaffold
chromene
been
facilely
synthesized
via
green
reactions
water
system.
These
compounds
show
regiostructure-,
polymorphism-
substituent-dependent
fluorescence,
which
clearly
illustrates
close
relationship
between
macroscopic
properties
hierarchical
structure
aggregates.
Due
superior
biocompatibility
scaffold,
chromene-based
can
specifically
target
endoplasmic
reticulum
(ER)
introduction
tosyl
amide.
This
work
provided
for
functional
basis
sustainable
‘in-water’
synthesis,
applicable
regiostructure-dependent
effective
ER-specific
imaging.
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(8), С. 2028 - 2041
Опубликована: Янв. 1, 2024
The
endoplasmic
reticulum
(ER)
and
lipid
droplets
(LDs)
intricately
interact
in
cellular
processes,
with
the
ER
serving
as
a
hub
for
synthesis
LDs
acting
storage
organelles
lipids.
Nano Letters,
Год журнала:
2024,
Номер
24(7), С. 2264 - 2272
Опубликована: Фев. 7, 2024
Developing
general
methods
to
fabricate
water-dispersible
and
biocompatible
fluorescent
probes
will
promote
different
biological
visualization
applications.
Herein,
we
report
a
metal-facilitated
method
ultrabright
green-emissive
nanodots
via
the
one-step
solvothermal
treatment
of
rose
bengal,
ethanol,
various
metal
ions.
These
metal-doped
show
good
water
dispersity,
ultrahigh
photoluminescence
quantum
yields
(PLQYs)
(e.g.,
PLQY
Fe-doped
(FeNDs)
was
∼97%),
low
phototoxicity.
Owing
coordination
effect
ions,
FeNDs
realize
glutathione
detection
with
outstanding
properties.
Benefiting
from
high
endoplasmic
reticulum
(ER)
affinity
chloride
group,
can
act
as
an
ER
tracker
long
imaging
capacity
(FeNDs:
>24
h;
commercial
tracker:
∼1
h)
superb
photostability
achieve
tissue
in
living
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(17)
Опубликована: Март 5, 2024
Realizing
protein
analysis
in
organelles
of
living
cells
is
great
significance
for
developing
diagnostic
and
therapeutic
methods
diseases.
Fluorescent-labeled
antibodies
with
well
imaging
performance
high
affinity
are
classical
biochemical
tools
analysis,
while
due
to
the
inability
effectively
enter
into
cells,
not
mention
uncontrollable
reaction
sites
that
might
cause
inactivation
when
chemically
modification,
they
hard
apply
cells.
Inspired
by
structure
fluorescent-labeled
antibodies,
we
designed
as
a
universal
detection
platform
was
based
on
peptide-conjugated
probes
(PCPs)
consisted
three
parts:
a)
rotor
type
fluorescent
molecular
scaffold
conjugation
signal
output;
b)
cell
penetration
recognition
unit;
c)
subcellular
organelle
targeting
unit.
In
PCPs
could
firstly
localize
at
then
proceed
specific
recognition,
thus
jointly
leading
restriction
twisted
intramolecular
charge
transfer
activation
fluorescence
signal.
As
proof-of-concept,
six
different
proteins
typical
intracellular
be
detected
our
through
simply
replacing
sequence
matching
units.
The
position
intensity
signals
demonstrated
specificity
universality
platform.
