Chemical Reviews,
Год журнала:
2023,
Номер
124(2), С. 554 - 628
Опубликована: Ноя. 22, 2023
In
vivo
imaging
technologies
have
emerged
as
a
powerful
tool
for
both
fundamental
research
and
clinical
practice.
particular,
luminescence
in
the
tissue-transparent
near-infrared
(NIR,
700–1700
nm)
region
offers
tremendous
potential
visualizing
biological
architectures
pathophysiological
events
living
subjects
with
deep
tissue
penetration
high
contrast
owing
to
reduced
light–tissue
interactions
of
absorption,
scattering,
autofluorescence.
The
distinctive
quantum
effects
nanocrystals
been
harnessed
achieve
exceptional
photophysical
properties,
establishing
them
promising
category
luminescent
probes.
this
comprehensive
review,
between
light
tissues,
well
advantages
NIR
imaging,
are
initially
elaborated.
Subsequently,
we
focus
on
achieving
improved
by
optimizing
performance
nanocrystal
fluorophores.
ingenious
design
strategies
probes
discussed,
along
their
respective
biomedical
applications
versatile
modalities.
Finally,
thought-provoking
reflections
challenges
prospects
future
translation
nanocrystal-based
wisely
provided.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 2, 2024
Abstract
Near-infrared-I/II
fluorescent
proteins
(NIR-I/II
FPs)
are
crucial
for
in
vivo
imaging,
yet
the
current
NIR-I/II
FPs
face
challenges
including
scarcity,
requirement
chromophore
maturation,
and
limited
emission
wavelengths
(typically
<
800
nm).
Here,
we
utilize
synthetic
protein-seeking
NIR-II
dyes
as
chromophores,
which
covalently
bind
to
tag
(e.g.,
human
serum
albumin,
HSA)
through
a
site-specific
nucleophilic
substitution
reaction,
thereby
creating
proof-of-concept
biomimetic
FPs.
This
chemogenic
strategy
can
be
accomplished
under
gentle
physiological
conditions
without
catalysis.
Proteomics
analysis
identifies
specific
binding
site
(Cys
477
on
DIII).
significantly
enhance
brightness
photostability,
while
improving
biocompatibility,
allowing
high-performance
lymphography
angiography.
is
universal
applicable
wide
range
of
spectrally
separated
real-time
visualization
multiple
biological
events.
Overall,
this
straightforward
approach
holds
potential
transform
protein-based
bioimaging
enables
in-situ
albumin
targeting
create
deep-tissue
imaging
live
organisms.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(16)
Опубликована: Март 5, 2024
Fluorescence
imaging
in
the
second
near-infrared
window
(NIR-II)
is
burgeoning
because
of
its
higher
fidelity
monitoring
physiological
and
pathological
processes
than
clinical
visible/the
fluorescence
imaging.
Notably,
heavily
dependent
on
agents.
So
far,
indocyanine
green,
one
polymethine
dyes,
with
good
biocompatibility
renal
clearance
only
dye
approved
by
Food
Drug
Administration,
but
it
shows
relatively
low
NIR-II
brightness.
Importantly,
tremendous
efforts
are
devoted
to
synthesizing
dyes
for
preclinically
clinically.
They
have
shown
feasibility
customization
structure
properties
fulfill
various
needs
therapy.
Herein,
a
timely
update
special
focus
molecular
design
strategies
fluorescent,
photoacoustic,
multimodal
imaging,
offered.
Furthermore,
progress
sensing
biomarkers
even
reporting
drug
release
illustrated.
Moreover,
imaging-guided
therapies
summarized
regarding
chemo-,
photothermal,
photodynamic,
approaches.
In
addition,
artificial
intelligence
pointed
out
potential
expedite
development.
This
comprehensive
review
will
inspire
interest
among
wide
audience
offer
handbook
people
an
dyes.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 17, 2025
Near-infrared
(NIR)-II
fluorescence
imaging-guided
photodynamic
therapy
(PDT)
has
shown
great
potential
for
precise
diagnosis
and
treatment
of
tumors
in
deep
tissues;
however,
its
performance
is
severely
limited
by
the
undesired
aggregation
photosensitizers
competitive
relationship
between
emission
reactive
oxygen
species
(ROS)
generation.
Herein,
we
report
an
example
anionic
pentamethine
cyanine
(C5T)
photosensitizer
high-performance
NIR-II
PDT.
Through
counterion
engineering
approach,
a
triphenylphosphine
cation
(Pco)
modified
with
oligoethylene
glycol
chain
synthesized
adopted
as
C5T,
which
can
effectively
suppress
excessive
disordered
resulting
C5T-Pco
optimizing
dye
amphipathicity
enhancing
cyanine-counterion
interactions.
Dynamic
tuning
characteristics
ROS
generation
achieved
at
aggregate
level,
impressive
type
I
under
760
nm
light
irradiation,
accompanied
efficient
excited
808
nm.
As
result,
excitation
wavelength
selective
PDT
been
successfully
demonstrated
tumor
therapeutics
female
mice.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(14), С. 17664 - 17674
Опубликована: Апрель 3, 2023
Acute
kidney
injuries
(AKI)
have
serious
short-term
or
long-term
complications
with
high
morbidity
and
mortality
rate,
thus
posing
great
health
threats.
Developing
high-performance
NIR-II
probes
for
noninvasive
in
situ
detection
of
AKI
via
fluorescent
optoacoustic
dual-mode
imaging
is
significance.
Yet
chromophores
often
feature
long
conjugation
hydrophobicity,
which
prevent
them
from
being
renal
clearable,
limiting
their
applications
the
diseases.
To
fully
exploit
advantageous
features
heptamethine
cyanine
dye,
while
overcoming
its
relatively
poor
photostability,
to
strive
design
a
probe
imaging,
herein,
we
developed
PEG3-HC-PB,
water
soluble,
biomarker
activatable
has
good
photostability.
As
probe,
fluorescence
(900-1200
nm)
quenched
due
existence
electron-pulling
phenylboronic
group
(responsive
element),
it
exhibits
weak
absorption
peak
at
830
nm.
Meanwhile,
presence
overexpressed
H2O2
region
case
AKI,
converted
phenylhydroxy
group,
enhances
emission
(600-900
eventually
produces
conspicuous
signals
imaging.
This
enables
contrast-agent-induced
ischemia/reperfusion-induced
mice
using
real-time
3D-MSOT
response
H2O2.
Hence,
this
can
be
used
as
practicable
tool
detecting
AKI;
additionally,
strategy
could
provide
insight
into
other
large-conjugation
multifarious
biological
applications.
