Nanocrystals for Deep-Tissue In Vivo Luminescence Imaging in the Near-Infrared Region

Chemical Reviews, Journal Year: 2023, Volume and Issue: 124(2), P. 554 - 628

Published: Nov. 22, 2023

In vivo imaging technologies have emerged as a powerful tool for both fundamental research and clinical practice. particular, luminescence in the tissue-transparent near-infrared (NIR, 700–1700 nm) region offers tremendous potential visualizing biological architectures pathophysiological events living subjects with deep tissue penetration high contrast owing to reduced light–tissue interactions of absorption, scattering, autofluorescence. The distinctive quantum effects nanocrystals been harnessed achieve exceptional photophysical properties, establishing them promising category luminescent probes. this comprehensive review, between light tissues, well advantages NIR imaging, are initially elaborated. Subsequently, we focus on achieving improved by optimizing performance nanocrystal fluorophores. ingenious design strategies probes discussed, along their respective biomedical applications versatile modalities. Finally, thought-provoking reflections challenges prospects future translation nanocrystal-based wisely provided.

Language: Английский

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Recent advances in the design and applications of near-infrared II responsive small molecule phototherapeutic agents

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 505, P. 215677 - 215677

Published: Jan. 31, 2024

Language: Английский

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Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 2, 2024

Abstract Near-infrared-I/II fluorescent proteins (NIR-I/II FPs) are crucial for in vivo imaging, yet the current NIR-I/II FPs face challenges including scarcity, requirement chromophore maturation, and limited emission wavelengths (typically < 800 nm). Here, we utilize synthetic protein-seeking NIR-II dyes as chromophores, which covalently bind to tag (e.g., human serum albumin, HSA) through a site-specific nucleophilic substitution reaction, thereby creating proof-of-concept biomimetic FPs. This chemogenic strategy can be accomplished under gentle physiological conditions without catalysis. Proteomics analysis identifies specific binding site (Cys 477 on DIII). significantly enhance brightness photostability, while improving biocompatibility, allowing high-performance lymphography angiography. is universal applicable wide range of spectrally separated real-time visualization multiple biological events. Overall, this straightforward approach holds potential transform protein-based bioimaging enables in-situ albumin targeting create deep-tissue imaging live organisms.

Language: Английский

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Development of Polymethine Dyes for NIR‐II Fluorescence Imaging and Therapy

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(16)

Published: March 5, 2024

Fluorescence imaging in the second near-infrared window (NIR-II) is burgeoning because of its higher fidelity monitoring physiological and pathological processes than clinical visible/the fluorescence imaging. Notably, heavily dependent on agents. So far, indocyanine green, one polymethine dyes, with good biocompatibility renal clearance only dye approved by Food Drug Administration, but it shows relatively low NIR-II brightness. Importantly, tremendous efforts are devoted to synthesizing dyes for preclinically clinically. They have shown feasibility customization structure properties fulfill various needs therapy. Herein, a timely update special focus molecular design strategies fluorescent, photoacoustic, multimodal imaging, offered. Furthermore, progress sensing biomarkers even reporting drug release illustrated. Moreover, imaging-guided therapies summarized regarding chemo-, photothermal, photodynamic, approaches. In addition, artificial intelligence pointed out potential expedite development. This comprehensive review will inspire interest among wide audience offer handbook people an dyes.

Language: Английский

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Small molecular cyanine dyes for phototheranostics

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 516, P. 215986 - 215986

Published: June 2, 2024

Language: Английский

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Biomedical applications of NIR-II organic small molecule fluorescent probes in different organs

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 519, P. 216122 - 216122

Published: Aug. 6, 2024

Language: Английский

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Recent advances in near-infrared-II organic J-aggregates for bio-applications

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 528, P. 216379 - 216379

Published: Jan. 4, 2025

Language: Английский

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Aggregation control of anionic pentamethine cyanine enabling excitation wavelength selective NIR-II fluorescence imaging-guided photodynamic therapy

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 17, 2025

Near-infrared (NIR)-II fluorescence imaging-guided photodynamic therapy (PDT) has shown great potential for precise diagnosis and treatment of tumors in deep tissues; however, its performance is severely limited by the undesired aggregation photosensitizers competitive relationship between emission reactive oxygen species (ROS) generation. Herein, we report an example anionic pentamethine cyanine (C5T) photosensitizer high-performance NIR-II PDT. Through counterion engineering approach, a triphenylphosphine cation (Pco) modified with oligoethylene glycol chain synthesized adopted as C5T, which can effectively suppress excessive disordered resulting C5T-Pco optimizing dye amphipathicity enhancing cyanine-counterion interactions. Dynamic tuning characteristics ROS generation achieved at aggregate level, impressive type I under 760 nm light irradiation, accompanied efficient excited 808 nm. As result, excitation wavelength selective PDT been successfully demonstrated tumor therapeutics female mice.

Language: Английский

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A TICT-based fluorescent probe for hypochlorous acid and its application to cellular and zebrafish imaging

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 392, P. 134041 - 134041

Published: May 29, 2023

Language: Английский

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Renal-Clearable Probe with Water Solubility and Photostability for Biomarker-Activatable Detection of Acute Kidney Injuries via NIR-II Fluorescence and Optoacoustic Imaging

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(14), P. 17664 - 17674

Published: April 3, 2023

Acute kidney injuries (AKI) have serious short-term or long-term complications with high morbidity and mortality rate, thus posing great health threats. Developing high-performance NIR-II probes for noninvasive in situ detection of AKI via fluorescent optoacoustic dual-mode imaging is significance. Yet chromophores often feature long conjugation hydrophobicity, which prevent them from being renal clearable, limiting their applications the diseases. To fully exploit advantageous features heptamethine cyanine dye, while overcoming its relatively poor photostability, to strive design a probe imaging, herein, we developed PEG3-HC-PB, water soluble, biomarker activatable has good photostability. As probe, fluorescence (900-1200 nm) quenched due existence electron-pulling phenylboronic group (responsive element), it exhibits weak absorption peak at 830 nm. Meanwhile, presence overexpressed H2O2 region case AKI, converted phenylhydroxy group, enhances emission (600-900 eventually produces conspicuous signals imaging. This enables contrast-agent-induced ischemia/reperfusion-induced mice using real-time 3D-MSOT response H2O2. Hence, this can be used as practicable tool detecting AKI; additionally, strategy could provide insight into other large-conjugation multifarious biological applications.

Language: Английский

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