bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 23, 2023
Summary
Dietary
nutrients
and
microbial
cross-feeding
allow
diverse
bacteria
to
colonize
the
animal
gut.
Less
is
known
about
role
of
host-derived
in
enabling
gut
bacterial
colonization.
We
examined
metabolic
interactions
within
evolutionary
ancient
symbiosis
between
honey
bee
(
Apis
mellifera
)
core
microbiota
member
Snodgrassella
alvi
.
This
Betaproteobacteria
incapable
metabolizing
saccharides,
yet
colonizes
presence
only
a
sugar
diet.
Using
comparative
metabolomics,
13
C
tracers,
Nanoscale
secondary
ion
mass
spectrometry
(NanoSIMS),
we
show
vivo
that
S.
grows
on
organic
acids,
including
citrate,
glycerate
3-hydroxy-3-methylglutarate
which
are
actively
secreted
by
host
into
lumen.
additionally
modulates
tryptophan
metabolism
converting
kynurenine
anthranilate.
These
results
suggest
adapted
specific
niche
depends
nutritional
resources.
Nature Microbiology,
Год журнала:
2024,
Номер
9(2), С. 477 - 489
Опубликована: Янв. 15, 2024
Diverse
bacteria
can
colonize
the
animal
gut
using
dietary
nutrients
or
by
engaging
in
microbial
crossfeeding
interactions.
Less
is
known
about
role
of
host-derived
enabling
bacterial
colonization.
Here
we
examined
metabolic
interactions
within
evolutionary
ancient
symbiosis
between
honey
bee
(Apis
mellifera)
and
core
microbiota
member
Snodgrassella
alvi.
This
betaproteobacterium
incapable
metabolizing
saccharides,
yet
colonizes
presence
a
sugar-only
diet.
Using
comparative
metabolomics,
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Май 20, 2023
Abstract
Nosema
ceranae
is
an
intracellular
parasite
invading
the
midgut
of
honeybees,
which
causes
serious
nosemosis
implicated
in
honeybee
colony
losses
worldwide.
The
core
gut
microbiota
involved
protecting
against
parasitism,
and
genetically
engineering
native
symbionts
provides
a
novel
efficient
way
to
fight
pathogens.
Here,
using
laboratory-generated
bees
mono-associated
with
members,
we
find
that
Snodgrassella
alvi
inhibit
microsporidia
proliferation,
potentially
via
stimulation
host
oxidant-mediated
immune
response.
Accordingly,
N.
employs
thioredoxin
glutathione
systems
defend
oxidative
stress
maintain
balanced
redox
equilibrium,
essential
for
infection
process.
We
knock
down
gene
expression
nanoparticle-mediated
RNA
interference,
targets
γ-glutamyl-cysteine
synthetase
reductase
genes
microsporidia.
It
significantly
reduces
spore
load,
confirming
importance
antioxidant
mechanism
invasion
parasite.
Finally,
modify
symbiotic
S.
deliver
dsRNA
corresponding
system
engineered
induces
interference
represses
expression,
thereby
inhibits
parasitism
significantly.
Specifically,
most
suppressed
by
recombinant
strain
or
mixture
bacteria
expressing
variable
dsRNA.
Our
findings
extend
our
previous
understanding
protection
provide
symbiont-mediated
RNAi
inhibiting
honeybees.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(33)
Опубликована: Авг. 9, 2023
Host
specificity
is
observed
in
gut
symbionts
of
diverse
animal
lineages.
But
how
hosts
maintain
while
rejecting
their
close
relatives
remains
elusive.
We
use
eusocial
bees
and
codiversified
bacteria
to
understand
host
regulation
driving
symbiotic
specificity.
The
cross-inoculation
bumblebee
Gilliamella
induced
higher
prostaglandin
the
honeybee
gut,
promoting
a
pronounced
response
through
immune
deficiency
(IMD)
Toll
pathways.
Gene
silencing
vitamin
C
treatments
indicate
that
reactive
oxygen
species
(ROS),
not
antimicrobial
peptides,
acts
as
effector
inhibiting
non-native
strain.
Quantitative
PCR
RNAi
further
reveal
regulatory
function
IMD
pathways,
which
Relish
dorsal-1
may
regulate
Dual
Oxidase
(
Duox
)
for
ROS
production.
Therefore,
maintains
by
creating
hostile
environment
exotic
bacteria,
differential
its
system,
reflecting
co-opting
existing
machinery
evolved
combat
pathogens.
Social
bees
harbor
conserved
gut
microbiotas
that
may
have
been
acquired
in
a
common
ancestor
of
social
and
subsequently
codiversified
with
their
hosts.
However,
most
this
knowledge
is
based
on
studies
the
honey
bumblebees.
Much
less
known
about
third
diverse
group
bees,
stingless
bees.
Specifically,
absence
genomic
data
from
presents
an
important
gap
understanding
evolution
functional
diversity
bee
microbiota.
Here,
we
combined
community
profiling
culturing
genome
sequencing
bacteria
six
neotropical
species
Brazil.
Phylogenomic
analyses
show
isolates
form
deep-branching
sister
clades
core
members
bumblebee
microbiota
capabilities,
confirming
ancestry
ecology
our
bacterial
phylogenies
were
not
congruent
those
host,
indicating
was
driven
by
strict
codiversification
but
included
host
switches
independent
symbiont
gain
losses.
Finally,
as
reported
for
microbiotas,
found
substantial
divergence
among
strains
bacteria,
suggesting
adaptation
to
different
glycan
niches.
Our
study
offers
first
insights
into
highlights
need
broader
samplings
understand
IMPORTANCE
Stingless
are
corbiculate
represent
pollinator
throughout
tropics
subtropics.
They
specialized
microbial
communities
related
bumblebees
likely
health.
Few
cultured
which
has
prevented
characterization
potential.
established
cultures
major
sequenced
genomes.
We
belong
novel
distantly
encoding
similar
capabilities.
new
perspective
basis
characterizing
symbiotic
relationships
between
Molecular Microbiology,
Год журнала:
2023,
Номер
122(3), С. 284 - 293
Опубликована: Сен. 17, 2023
Abstract
Honey
bees
have
emerged
as
a
new
model
to
study
the
gut–brain
axis,
they
exhibit
complex
social
behaviors
and
cognitive
abilities,
while
experiments
with
gnotobiotic
revealed
that
their
gut
microbiota
alters
both
brain
behavioral
phenotypes.
Furthermore,
honey
bee
functions
supporting
broad
range
of
been
intensively
studied
for
over
50
years,
has
experimentally
characterized
only
recently.
Here,
we
combined
six
published
datasets
from
metabolomic
analyses
provide
an
overview
neuroactive
metabolites
whose
abundance
in
gut,
hemolymph
varies
presence
microbiota.
Such
may
either
be
produced
by
bacteria,
released
pollen
grains
during
decomposition
or
other
organs
response
different
bacterial
products.
We
describe
current
state
knowledge
regarding
impact
such
on
function
behavior
further
hypotheses
explore
this
emerging
field
research.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(44)
Опубликована: Окт. 23, 2024
The
Type
VI
Secretion
System
(T6SS)
is
a
sophisticated
mechanism
utilized
by
gram-negative
bacteria
to
deliver
toxic
effector
proteins
into
target
cells,
influencing
microbial
community
dynamics
and
host
interactions.
In
this
study,
we
investigated
the
role
of
T6SSs
in
Snodgrassella
alvi
wkB2,
core
bacterial
symbiont
honey
bee
gut
microbiota.
We
generated
single-
double-knockout
mutants
targeting
essential
genes
(
tssD
tssE
)
both
T6SS-1
T6SS-2
assessed
their
colonization
competition
capabilities
vivo.
Our
results
indicate
that
are
nonessential
for
gut,
although
mutant
strains
exhibited
significantly
lower
levels
compared
wild-type
(WT)
strain.
Further,
defined
experiment
showed
S.
wkB2
do
not
impact
interspecific
among
bacteria.
However,
cocolonization
experiments
with
closely
related
demonstrated
plays
mediating
intraspecific
competition.
Transcriptomic
analysis
guts
monocolonized
WT
or
T6SS
revealed
differential
expression
immunity-related
relative
microbiota-deprived
bees,
such
as
upregulation
antimicrobial
peptide
apidaecin
presence
defensin
,
suggesting
contribute
shaping
immune
responses.
These
findings
provide
insight
ecological
roles
microbiota,
emphasizing
importance
maintaining
competitive
host–bacterial
To
understand
mechanisms
of
adaptation
and
plasticity
pollinators
other
insects
a
better
understanding
diversity
function
their
key
symbionts
is
required.
Commensalibacter
genus
acetic
acid
bacterial
in
the
gut
honey
bees
insect
species,
yet
little
information
available
on
bacteria.
In
present
study,
whole-genome
sequences
12
isolates
from
bumble
bees,
butterflies,
Asian
hornets
rowan
berries
were
determined,
publicly
genome
assemblies
14
strains
used
phylogenomic
comparative
genomic
analysis.