Foraging on host synthesized metabolites enables the bacterial symbiontSnodgrassella alvito colonize the honey bee gut DOI Open Access
Andrew Quinn, Yassine El Chazli, Stéphane Escrig

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 23, 2023

Summary Dietary nutrients and microbial cross-feeding allow diverse bacteria to colonize the animal gut. Less is known about role of host-derived in enabling gut bacterial colonization. We examined metabolic interactions within evolutionary ancient symbiosis between honey bee ( Apis mellifera ) core microbiota member Snodgrassella alvi . This Betaproteobacteria incapable metabolizing saccharides, yet colonizes presence only a sugar diet. Using comparative metabolomics, 13 C tracers, Nanoscale secondary ion mass spectrometry (NanoSIMS), we show vivo that S. grows on organic acids, including citrate, glycerate 3-hydroxy-3-methylglutarate which are actively secreted by host into lumen. additionally modulates tryptophan metabolism converting kynurenine anthranilate. These results suggest adapted specific niche depends nutritional resources.

Language: Английский

The honeybee microbiota and its impact on health and disease DOI
Erick V. S. Motta, Nancy A. Moran

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(3), P. 122 - 137

Published: Dec. 4, 2023

Language: Английский

Citations

77
Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi DOI Creative Commons
Andrew Quinn, Yassine El Chazli, Stéphane Escrig

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(2), P. 477 - 489

Published: Jan. 15, 2024

Diverse bacteria can colonize the animal gut using dietary nutrients or by engaging in microbial crossfeeding interactions. Less is known about role of host-derived enabling bacterial colonization. Here we examined metabolic interactions within evolutionary ancient symbiosis between honey bee (Apis mellifera) and core microbiota member Snodgrassella alvi. This betaproteobacterium incapable metabolizing saccharides, yet colonizes presence a sugar-only diet. Using comparative metabolomics,

Language: Английский

Citations

23
Engineered symbiotic bacteria interfering Nosema redox system inhibit microsporidia parasitism in honeybees DOI Creative Commons
Haoyu Lang, Hao Wang, Haoqing Wang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 20, 2023

Abstract Nosema ceranae is an intracellular parasite invading the midgut of honeybees, which causes serious nosemosis implicated in honeybee colony losses worldwide. The core gut microbiota involved protecting against parasitism, and genetically engineering native symbionts provides a novel efficient way to fight pathogens. Here, using laboratory-generated bees mono-associated with members, we find that Snodgrassella alvi inhibit microsporidia proliferation, potentially via stimulation host oxidant-mediated immune response. Accordingly, N. employs thioredoxin glutathione systems defend oxidative stress maintain balanced redox equilibrium, essential for infection process. We knock down gene expression nanoparticle-mediated RNA interference, targets γ-glutamyl-cysteine synthetase reductase genes microsporidia. It significantly reduces spore load, confirming importance antioxidant mechanism invasion parasite. Finally, modify symbiotic S. deliver dsRNA corresponding system engineered induces interference represses expression, thereby inhibits parasitism significantly. Specifically, most suppressed by recombinant strain or mixture bacteria expressing variable dsRNA. Our findings extend our previous understanding protection provide symbiont-mediated RNAi inhibiting honeybees.

Language: Английский

Citations

35
Reactive oxygen species are regulated by immune deficiency and Toll pathways in determining the host specificity of honeybee gut bacteria DOI Creative Commons

Lizhen Guo,

Junbo Tang, Min Tang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(33)

Published: Aug. 9, 2023

Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.

Language: Английский

Citations

34
Strain diversity and host specificity of the gut symbiont Gilliamella in Apis mellifera, Apis cerana and Bombus terrestris DOI

Nihong Zhou,

Qi Zheng,

Yao Liu

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128048 - 128048

Published: Jan. 6, 2025

Language: Английский

Citations

2
Deep Divergence and Genomic Diversification of Gut Symbionts of Neotropical Stingless Bees DOI Creative Commons
Garance Sarton-Lohéac, Carlos Gustavo Nunes-Silva, Florent Mazel

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: March 20, 2023

Social bees harbor conserved gut microbiotas that may have been acquired in a common ancestor of social and subsequently codiversified with their hosts. However, most this knowledge is based on studies the honey bumblebees. Much less known about third diverse group bees, stingless bees. Specifically, absence genomic data from presents an important gap understanding evolution functional diversity bee microbiota. Here, we combined community profiling culturing genome sequencing bacteria six neotropical species Brazil. Phylogenomic analyses show isolates form deep-branching sister clades core members bumblebee microbiota capabilities, confirming ancestry ecology our bacterial phylogenies were not congruent those host, indicating was driven by strict codiversification but included host switches independent symbiont gain losses. Finally, as reported for microbiotas, found substantial divergence among strains bacteria, suggesting adaptation to different glycan niches. Our study offers first insights into highlights need broader samplings understand IMPORTANCE Stingless are corbiculate represent pollinator throughout tropics subtropics. They specialized microbial communities related bumblebees likely health. Few cultured which has prevented characterization potential. established cultures major sequenced genomes. We belong novel distantly encoding similar capabilities. new perspective basis characterizing symbiotic relationships between

Language: Английский

Citations

21
Honey bee functional genomics using symbiont-mediated RNAi DOI
Patrick J. Lariviere, Sean P. Leonard, Richard D. Horak

et al.

Nature Protocols, Journal Year: 2022, Volume and Issue: 18(3), P. 902 - 928

Published: Dec. 2, 2022

Language: Английский

Citations

26
Neuroactive metabolites modulated by the gut microbiota in honey bees DOI Creative Commons
Amélie Cabirol, Silvia Moriano‐Gutierrez, Philipp Engel

et al.

Molecular Microbiology, Journal Year: 2023, Volume and Issue: 122(3), P. 284 - 293

Published: Sept. 17, 2023

Abstract Honey bees have emerged as a new model to study the gut–brain axis, they exhibit complex social behaviors and cognitive abilities, while experiments with gnotobiotic revealed that their gut microbiota alters both brain behavioral phenotypes. Furthermore, honey bee functions supporting broad range of been intensively studied for over 50 years, has experimentally characterized only recently. Here, we combined six published datasets from metabolomic analyses provide an overview neuroactive metabolites whose abundance in gut, hemolymph varies presence microbiota. Such may either be produced by bacteria, released pollen grains during decomposition or other organs response different bacterial products. We describe current state knowledge regarding impact such on function behavior further hypotheses explore this emerging field research.

Language: Английский

Citations

15
Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont DOI Creative Commons
Erick V. S. Motta, Patrick J. Lariviere, Korin Rex Jones

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(44)

Published: Oct. 23, 2024

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in Snodgrassella alvi wkB2, core bacterial symbiont honey bee gut microbiota. We generated single- double-knockout mutants targeting essential genes ( tssD tssE ) both T6SS-1 T6SS-2 assessed their colonization competition capabilities vivo. Our results indicate that are nonessential for gut, although mutant strains exhibited significantly lower levels compared wild-type (WT) strain. Further, defined experiment showed S. wkB2 do not impact interspecific among bacteria. However, cocolonization experiments with closely related demonstrated plays mediating intraspecific competition. Transcriptomic analysis guts monocolonized WT or T6SS revealed differential expression immunity-related relative microbiota-deprived bees, such as upregulation antimicrobial peptide apidaecin presence defensin , suggesting contribute shaping immune responses. These findings provide insight ecological roles microbiota, emphasizing importance maintaining competitive host–bacterial

Language: Английский

Citations

5
A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont DOI Creative Commons
Juliana Botero, Atena Sadat Sombolestani, Margo Cnockaert

et al.

Animal Microbiome, Journal Year: 2023, Volume and Issue: 5(1)

Published: April 29, 2023

To understand mechanisms of adaptation and plasticity pollinators other insects a better understanding diversity function their key symbionts is required. Commensalibacter genus acetic acid bacterial in the gut honey bees insect species, yet little information available on bacteria. In present study, whole-genome sequences 12 isolates from bumble bees, butterflies, Asian hornets rowan berries were determined, publicly genome assemblies 14 strains used phylogenomic comparative genomic analysis.

Language: Английский

Citations

10